Quantum dots and duplex-specific nuclease enabled ultrasensitive detection and serotyping of Dengue viruses in one step in a single tube

Shen, Wei; Gao, Zhiqiang

doi:10.1016/j.bios.2014.10.060

Cited by 13 publications

(8 citation statements)

References 22 publications

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“…Shen et al developed a fluorescent DNA biosensor to detect four types of DENV using a quantum dot-capped DNA capture probe (QD-CPs) ( Figure 6 a) [ 171 ]. In this biosensor, DNA capture probes bind to the surface of quantum dots and magnetic beads.…”

Section: Dna Nanotechnology-enabled Biosensor For Virus Detectionmentioning

confidence: 99%

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Recent Advances in DNA Nanotechnology-Enabled Biosensors for Virus Detection

2023

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Virus-related infectious diseases are serious threats to humans, which makes virus detection of great importance. Traditional virus-detection methods usually suffer from low sensitivity and specificity, are time-consuming, have a high cost, etc. Recently, DNA biosensors based on DNA nanotechnology have shown great potential in virus detection. DNA nanotechnology, specifically DNA tiles and DNA aptamers, has achieved atomic precision in nanostructure construction. Exploiting the programmable nature of DNA nanostructures, researchers have developed DNA nanobiosensors that outperform traditional virus-detection methods. This paper reviews the history of DNA tiles and DNA aptamers, and it briefly describes the Baltimore classification of virology. Moreover, the advance of virus detection by using DNA nanobiosensors is discussed in detail and compared with traditional virus-detection methods. Finally, challenges faced by DNA nanobiosensors in virus detection are summarized, and a perspective on the future development of DNA nanobiosensors in virus detection is also provided.

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Section: Dna Nanotechnology-enabled Biosensor For Virus Detectionmentioning

confidence: 99%

“… Fluorescence-based DNA nanobiosensors for virus detection. ( a ) Schematic representation of the working principle of the fluorescence DENV assay based on QD-CPs [ 171 ]. Copyright 2015, Elsevier.…”

Section: Dna Nanotechnology-enabled Biosensor For Virus Detectionmentioning

confidence: 99%

Recent Advances in DNA Nanotechnology-Enabled Biosensors for Virus Detection

2023

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“…Recently, quantum dot-based biosensors have been extensively studied in the field of human viruses ( Adegoke et al, 2016 ; Shamsipur et al, 2017 ; Shen and Gao, 2015 ) because not only do quantum dots not require the expensive equipments that SPR or electrochemical biosensors do, but they can also reduce the non-specificity due to their own fluorescent material in the host cell. However, their application to the field of plant virus detection has only begun recently and only a very few cases have been reported ( Khater et al, 2017 ).…”

Section: Detection Of Plant Viruses Using Biosensorsmentioning

confidence: 99%

The Current Status and Future Outlook of Quantum Dot-Based Biosensors for Plant Virus Detection

Hong

Lee

2018

Plant Pathol J

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“…In recent years, fluorescence-based nanosensors have received tremendous attention in biological detection and diagnosis [ 9 ]. As a class of novel fluorescent nanoparticles, quantum dots (QDs) have been widely investigated for the rapid and sensitive detection of various pathogens such as infectious bursal disease virus [ 10 ], avian leukosis virus [ 11 ], Peste des petits ruminants virus [ 12 ], Ectromelia virus [ 13 ], Hepatitis B virus [ 14 ], Dengue virus [ 15 ], etc. It is worth quoting that QDs are gradually becoming ideal donors in fluorescence analysis due to their captivating optical characteristics of high luminous intensity, large Stokes shift, wider excitation spectra, symmetry, tunable, and narrow emission spectrum [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

A Rapid, Fluorescence Switch-On Biosensor for Early Diagnosis of Sorghum Mosaic Virus

et al. 2022

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For the first time, a nanobiosensor was established for Sorghum mosaic virus (SrMV) detection. The biosensor consists of cadmium telluride quantum dots (CdTe QDs) conjugated to the specific antibody (Ab) against SrMV coat protein (CP) and carbon quantum dots (C QDs) labeled with SrMV coat protein. The formation of the fluorophore-quencher immunocomplex CdTe QDs-Ab+C QDs-CP led to a distinct decrease in the fluorescence intensity of CdTe QDs. Conversely, the emission intensity of CdTe QDs recovered upon the introduction of unlabeled CP. The developed biosensor showed a limit of detection of 44 nM in a linear range of 0.10–0.54 μM and exhibited the strongest fluorescence intensity (about 47,000 a.u.) at 552 nm. This strategy was applied to detect purified CP in plant sap successfully with a recovery rate between 93–103%. Moreover, the feasibility of the proposed method was further verified by the detection of field samples, and the results were consistent with an enzyme-linked immunosorbent assay (ELISA). Contrarily to ELISA, the proposed biosensor did not require excessive washing and incubation steps, thus the detection could be rapidly accomplished in a few minutes. The high sensitivity and short assay time of this designed biosensor demonstrated its potential application in situ and rapid detection. In addition, the fluorescence quenching of CdTe QDs was attributed to dynamic quenching according to the Stern-Volmer equation.

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Quantum dots and duplex-specific nuclease enabled ultrasensitive detection and serotyping of Dengue viruses in one step in a single tube

Cited by 13 publications

References 22 publications

Recent Advances in DNA Nanotechnology-Enabled Biosensors for Virus Detection

Recent Advances in DNA Nanotechnology-Enabled Biosensors for Virus Detection

The Current Status and Future Outlook of Quantum Dot-Based Biosensors for Plant Virus Detection

A Rapid, Fluorescence Switch-On Biosensor for Early Diagnosis of Sorghum Mosaic Virus

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