We report the 1-D cooling of 85 Rb atoms using a velocity-dependent optical force based upon Ramsey matter-wave interferometry. Using stimulated Raman transitions between ground hyperfine states, 12 cycles of the interferometer sequence cool a freely-moving atom cloud from 21 µK to 3 µK. This pulsed analog of continuous-wave Doppler cooling is effective at temperatures down to the recoil limit; with augmentation pulses to increase the interferometer area, it should cool more quickly than conventional methods, and be more suitable for species that lack a closed radiative transition.The laser cooling of atomic gases has revolutionized experimental atomic physics [1] and raised the prospect of a range of atomic quantum technologies [2,3]. However, traditional Doppler cooling [4,5] relies upon the velocity-dependence of a single narrow radiative transition, and spontaneous emission to reset the atomic state. The cooling force is limited to a half photon-impulse per excited-state lifetime and, as many impulses are needed, requires a transition that can be closed by a few repump lasers. Doppler cooling has thus so far been limited to a handful of atomic elements and molecules [6][7][8][9].In [10], Weitz and Hänsch proposed a mechanism that could extend laser cooling to a wider range of species, by replacing the continuous wave (CW) excitation of conventional Doppler cooling with the broadband laser pulses of Ramsey matter-wave interferometry, and interleaving inversion pulses to eliminate the dependence upon the internal state energies. The interference signal, and hence the impulse imparted, were thus determined only by the particle's kinetic energy; manifold transitions could be accessed and, while spontaneous emission remained the entropy-removing mechanism, various schemes [11][12][13] could increase the impulse per spontaneous event. With a drive towards efficient pulsed schemes for molecular cooling [14][15][16] supported by improved mode-locked laser technologies, interferometric cooling appears a promising and flexible tool.The idea of a pulsed Ramsey analog to CW Doppler cooling has until now remained untested. In this letter, we report the first experimental demonstration of 1-D interferometric cooling of a cloud of already ultracold Rb atoms. Our long-lived quasi-two-level system, comprising the two 5S 1/2 ground hyperfine states of 85 Rb between which we drive stimulated Raman transitions, in principle allows cooling to the recoil limit, and we show that with just 12 cycles of the interferometric cooling sequence the atom cloud is cooled from 21 ± 2 µK to 3.2 ± 0.4 µK. Relaxation after each cycle is achieved by rapid pumping and decay of the single-photon 5S 1/2 -5P 3/2 transition, and the cooling rate is therefore limited mainly by the time needed for interferometric resolution of the different velocity classes.The Raman interferometric cooling mechanism is as follows. Two π/2 laser pulses, separated by a dwell time τ , act upon a two-level atom |Ψ = c 1 |1 + c 2 |2 as the beamsplitter and combiner of a R...