“…1A). Once produced, C 30 botryococcene is further metabolized by methylation at carbons 3, 7, 16, and 20 to produce C 31 , C 32 , C 33 , and C 34 botryococcenes (19,43,44), and even further methylated to C 36 and C 37 botryococcenes in some B race strains (22,45). In C 34 botryococcene-producing strains such as the Berkeley (Showa) strain, the majority (Ͼ99%) of the C 34 botryococcenes exist in the extracellular matrix whereas the intracellular oil comprises predominantly the lower carbon number botryococcenes (19,20).…”