Quaternized starch-based carrier for siRNA delivery: From cellular uptake to gene silencing

Amar-Lewis, Eliz; Azagury, Aharon; Chintakunta, Ramesh; Goldbart, Riki; Traitel, Tamar; Prestwood, Jackson; Landesman-Milo, Dalit; Peer, Dan; Kost, Joseph

doi:10.1016/j.jconrel.2014.04.031

Cited by 56 publications

(41 citation statements)

References 46 publications

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“…Compared to amylose, CA showed a new peak at 3.09 ppm, which could be assigned to the –N + (CH 3 ) 3 [18]. This indicates that 3-chloro-2-hydroxypropyl trimethylammonium chloride was conjugated with amylose.…”

Section: Resultsmentioning

confidence: 99%

Magnetic Cationic Amylose Nanoparticles Used to Deliver Survivin-Small Interfering RNA for Gene Therapy of Hepatocellular Carcinoma In Vitro

Zhang

et al. 2017

Nanomaterials

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Amylose is a promising nanocarrier for gene delivery in terms of its good biocompatibility and high transfection efficiency. Small interfering RNA against survivin (survivin-siRNA) can cause tumor apoptosis by silencing a hepatocellular carcinoma (HCC)-specific gene at the messenger RNA level. In this study, we developed a new class of folate-functionalized, superparamagnetic iron oxide (SPIO)-loaded cationic amylose nanoparticles to deliver survivin-siRNA to HCC cells. The cellular uptake of nanocomplexes, cytotoxicity, cell apoptosis, and gene suppression mediated by siRNA-complexed nanoparticles were tested. The results demonstrated that folate-functionalized, SPIO-loaded cationic amylose nanoparticles can mediate a specific and safe cellular uptake of survivin-siRNA with high transfection efficiency, resulting in a robust survivin gene downregulation in HCC cells. The biocompatible complex of cationic amylose could be used as an efficient, rapid, and safe gene delivery vector. Upon SPIO loading, it holds a great promise as a theranostic carrier for gene therapy of HCC.

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Section: Resultsmentioning

confidence: 99%

Magnetic Cationic Amylose Nanoparticles Used to Deliver Survivin-Small Interfering RNA for Gene Therapy of Hepatocellular Carcinoma In Vitro

Zhang

et al. 2017

Nanomaterials

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“…After mixing of the two phases, stable water-in-oil micelle dispersions will be obtained, which can be subsequently freeze-dried by flashfreezing, and result in AOT-coated sugar-glass nanoparticles. 157 Antibacterial nanodevices are interesting for coatings and wound dressings if the release of antibacterial agents can be triggered by the presence of bacteria. The trehalose, which transfers into the gel phase under dehydrating conditions, protects the cell internal organelles and hence protect the cells in desiccation and serves to protect the protein from chemical and physical degradation during storage.…”

Section: Carbohydrate-constructed Nanocarriersmentioning

confidence: 99%

Carbohydrate nanocarriers in biomedical applications: functionalization and construction

et al. 2015

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The specific targeting of either tumor cells or immune cells in vivo by carefully designed and appropriately surface-functionalized nanocarriers may become an effective therapeutic treatment for a variety of diseases. Carbohydrates, which are prominent biomolecules, have shown their outstanding ability in balancing the biocompatibility, stability, biodegradability, and functionality of nanocarriers. The recent applications of sugar (mono/oligosaccharides and/or polysaccharides) for the development of nanomedicines are summarized in this review, including the application of carbohydrates for the surface-functionalization of various nanocarriers and for the construction of the nanocarrier itself. Current problems and challenges are also addressed.

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“…A viable non-viral vector should be able to protect genes from complex biological environment, improve cellular uptake and facilitate endosomal escape. Due to the anionic nature of genes, various synthetic cationic polymers, such as poly(ethyleneimine) (PEI), [51] poly(amido amine) (PAA), [52] poly(β-amino ester) (PAE), [53] polypeptide, [54] poly(dimethylaminoethyl methacrylate) (PDMAEMA) [55] and some natural polymers, such as chitosan [56] and cationic starches [57] have been studied and used to form complex with genes through electrostatic interactions to fabricate gene delivery systems.…”

Section: Co-delivery Of Chemical Drugs and Genesmentioning

confidence: 99%

Co‐Delivery of Drugs and Genes Using Polymeric Nanoparticles for Synergistic Cancer Therapeutic Effects

Thambi

Lee

2017

Adv Healthcare Materials

107

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Drug and gene delivery systems based on nanoparticles, microparticles and hydrogels have been widely studied for cancer treatment in the past decade. To achieve an efficient and safe delivery, selection of drug and gene delivery carrier is critical. Biocompatible polymeric nanoparticles are considerably promising carrier candidates in delivery of drugs and genes because of their unique chemical and physical properties. However, delivery of a drug or gene sometimes cannot achieve a satisfactory treatment effect. Therefore, co‐delivery of dual drugs or co‐delivery of a drug and a gene in a polymeric nanoparticle has attracted attention. Such co‐delivery systems can overcome multi‐drug resistance of chemical drugs and achieve a synergistic therapeutic effect. In this progress report, we summarize recent progress in the preparation and application of polymeric drug and gene co‐delivery nanosystems. The remaining challenges and future trends in this field are also included.

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Quaternized starch-based carrier for siRNA delivery: From cellular uptake to gene silencing

Cited by 56 publications

References 46 publications

Magnetic Cationic Amylose Nanoparticles Used to Deliver Survivin-Small Interfering RNA for Gene Therapy of Hepatocellular Carcinoma In Vitro

Magnetic Cationic Amylose Nanoparticles Used to Deliver Survivin-Small Interfering RNA for Gene Therapy of Hepatocellular Carcinoma In Vitro

Carbohydrate nanocarriers in biomedical applications: functionalization and construction

Co‐Delivery of Drugs and Genes Using Polymeric Nanoparticles for Synergistic Cancer Therapeutic Effects

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