Quebrachitol inhibits biofilm formation and virulence production against methicillin-resistant Staphylococcus aureus

Vijayakumar, K. R.; Bharathidasan, Veeraiyan; Manigandan, Vajravelu; Danaraj, Jeyapragash

doi:10.1016/j.micpath.2020.104286

Cited by 17 publications

(8 citation statements)

References 59 publications

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“…Biofilm formation is followed by adhesion to the surface, auto-aggregation, attachment via hydrophobic interactions, and EPS production [ 3 , 32 , 33 ]. Therefore, CFS and GSE affected all steps of biofilm formation: 1) the initial step of adhesion, attachment, and biofilm formation of S. aureus ; 2) the step of attachment by auto-aggregation and establishment of hydrophobic interactions; and 3) the step of maturation by EPS production.…”

Section: Resultsmentioning

confidence: 99%

“…Polysaccharide intercellular adhesin (PIA), a component of the polysaccharide slime of S. aureus , is synthesized by the intercellular adhesion ( ica ) operon and plays an important role in biofilm formation and adhesion [ 34 ]. The ica operon consists of the icaABCD cluster and icaR ; among operon components, icaA is the essential regulator of icaD expression, which mediates PIA biosynthesis [ 32 ].…”

Section: Resultsmentioning

confidence: 99%

“…The virulence genes accessory gene regulator ( agrA ) and staphylococcal accessory regulator ( sarA ) also affect biofilm formation [ 8 ]. In fact, agrA and sarA regulate the quorum sensing system and the secretion of adhesion factors, thereby inhibiting initial adhesion and cell-cell interactions, respectively [ 31 , 32 ]. Notably, sigB modulates the expression of agrA and sarA during stress response [ 32 ].…”

Section: Resultsmentioning

confidence: 99%

“…In fact, agrA and sarA regulate the quorum sensing system and the secretion of adhesion factors, thereby inhibiting initial adhesion and cell-cell interactions, respectively [ 31 , 32 ]. Notably, sigB modulates the expression of agrA and sarA during stress response [ 32 ].…”

Section: Resultsmentioning

confidence: 99%

See 3 more Smart Citations

Anti-Biofilm Activity of Cell-Free Supernatant of Saccharomyces cerevisiae against Staphylococcus aureus

Kim

Park

et al. 2020

J. Microbiol. Biotechnol.

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Foodborne diseases caused by pathogens pose a great threat to human health. According to the Center for Disease Control and Prevention (CDC), foodborne diseases are caused by Salmonella spp., Clostridium perfringens, Campylobacter spp., and Staphylococcus aureus, the most common microorganisms found in food eaten in the United States [1]. Most foodborne pathogens can form biofilms on various surfaces, such as stainless steel, glass, polyurethane, and rubber [2,3]. Biofilms formed by foodborne pathogens have become a major problem in meat, dairy, and fish processing, as well as in the ready-to-eat (RTE) food industry. Biofilm formation is an important factor in the cross-contamination and persistence of foodborne pathogens [4]. It is difficult to remove foodborne pathogens once they form biofilms because biofilm cells are more resistant to antimicrobial agents and sanitizing treatments than planktonic cells [5]. Biofilms are surrounded by extracellular polymeric substances, which are composed of exopolysaccharides (EPS), proteins, lipids, and extracellular DNA [6]. Extracellular polymeric substances protect bacterial cells from the effects of antimicrobial agents and assist in retaining the structural integrity of the biofilm. S. aureus is a major foodborne pathogen that causes 241,148 illnesses per year [7] and forms biofilms on food and food contact surfaces [8]. Moreover, biofilm formation by S. aureus plays a crucial role in staphylococcal infections by protecting the colony from the host immune system and antimicrobial treatment [9]. Therefore, the prevention of S. aureus biofilm formation has become important for countering foodborne staphylococcal outbreaks. Since antibiotics are limited in their ability to inhibit S. aureus biofilms, many studies on natural antimicrobial agents and compounds produced using probiotics have been performed [10-13]. For example, some natural preservatives, such as grapefruit seed extract (GSE), have been used together with antimicrobial and anti-biofilm agents against Gram-positive and Gram-negative bacteria [14]. Moreover, some probiotics consisting of lactic acid bacteria have been reported to inhibit biofilm formation by Listeria monocytogenes [4], Pseudomonas aeruginosa [15], Bacillus licheniformis [16], Vibrio spp. [17], and methicillin-resistant S. aureus (MRSA) [18]. Saccharomyces cerevisiae has been reported that the probiotic yeast isolated from fermented food such as kefir, wine, and cucumber jangajji (Korean fermented food) [19,20]. The positive effects of S. cerevisiae, including antioxidant, anti-inflammatory, toxin eradication, and antagonistic activities, have been investigated [21-23]. In particular, the supernatant and lysate of S. cerevisiae cultures exerted an anti-biofilm effect against S. aureus, as demonstrated by the expression of the α-hemolysin and enterotoxin A genes (hla and sea). However, the effects of S. cerevisiae on the cell surface characteristics, EPS production, and expression of biofilm-related genes in S. aureus have not yet been investigat...

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

See 2 more Smart Citations

Anti-Biofilm Activity of Cell-Free Supernatant of Saccharomyces cerevisiae against Staphylococcus aureus

Kim

Park

et al. 2020

J. Microbiol. Biotechnol.

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“…The antibio lm potential of honey-L. plantarum formulation was assayed using 24 well microtiter plates as described in a previous study with minor modi cations (Vijayakumar et al 2020). Brie y, honey-L.…”

Section: Bio Lm Formation Inhibition Assaymentioning

confidence: 99%

Synergistic inhibitory effect of honey and Lactobacillus plantarum on pathogenic bacteria and their promotion of healing in infected wounds

Xiao

Su³

et al. 2022

Preprint

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Prevention and control of infections have become a formidable challenge due to the increasing resistance of pathogens to antibiotics. Probiotics have been discovered to have positive effects on the host, and it is well-known that some Lactobacilli are effective in treating and preventing inflammatory and infectious diseases. In this study, we developed an antibacterial formulation consisting of honey and Lactobacillus plantarum (honey-L. plantarum). The optimal formulation of honey (10%) and L. plantarum (1×109 CFU/mL) was used to investigate its antimicrobial effect and mechanism in vitro, and its healing effect on wound healing of whole skin infections in rats. Biofilm crystalline violet staining and fluorescent staining results indicated that the honey-L. plantarum formulation prevented the biofilm formation in Staphylococcus aureus (S. aureus) and Pseudomonas aeruginosa (P. aeruginosa) and increased the number of dead bacteria in the biofilms. Further mechanism studies revealed that the honey-L. plantarum formulation may inhibit biofilm formation by upregulating biofilm-related genes (icaA, icaR, sigB, sarA, and agrA) and downregulating quorum sensing (QS) associated genes (lasI, lasR, rhlI, rhlR, and pqsR). The growth curve implied that L. plantarum may have used honey to produce active antibacterial substances. Furthermore, the honey-L. plantarum formulation decreased the number of bacteria in the infected wounds of rats and accelerated the formation of new connective tissue to promote wound healing. Our study suggests that the honey-L. plantarum formulation provides a promising option for the treatment of pathogenic infections and wound healing.

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Anti-quorum sensing and antibiofilm potential of 1,8-cineole derived from Musa paradisiaca against Pseudomonas aeruginosa strain PAO1

Vijayakumar

Thirunanasambandham

Gunasekaran

2021

World J Microbiol Biotechnol

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Quebrachitol inhibits biofilm formation and virulence production against methicillin-resistant Staphylococcus aureus

Cited by 17 publications

References 59 publications

Anti-Biofilm Activity of Cell-Free Supernatant of Saccharomyces cerevisiae against Staphylococcus aureus

Anti-Biofilm Activity of Cell-Free Supernatant of Saccharomyces cerevisiae against Staphylococcus aureus

Synergistic inhibitory effect of honey and Lactobacillus plantarum on pathogenic bacteria and their promotion of healing in infected wounds

Anti-quorum sensing and antibiofilm potential of 1,8-cineole derived from Musa paradisiaca against Pseudomonas aeruginosa strain PAO1

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