Quercetin alleviated H₂O₂‐induced apoptosis and steroidogenic impairment in goat luteinized granulosa cells

Abstract

Quercetin (Que) is a natural flavonoid in most plants. Luteinized granulosa cell (LGC) culture is necessary for the study of follicle growth/differentiation. In the present study, we analyzed the role of Que in steroid production and apoptosis in hydrogen peroxide (H2O2)‐treated goat LGCs. The results showed that treatment with H2O2 induced apoptosis in goat LGCs, and treatment with Que decreased LGC apoptosis induced by H2O2 (P < .05), accompanied with the different expressions of BAX, BCL‐2, Caspase 3, and Cleaved caspase 3. Meanwhile, the messenger RNA expressions of nuclear factor erythroid 2 like 2 (Nrf2) and its downstream genes were upregulated with H2O2 +Que treatment, accompanied by the increased cellular viability (P < .05). Furthermore, Que alleviated H2O2‐induced reduction in the secretion of progesterone (P4) (P < .05); however, it had no effect on the secretion of estrogen (E2). Simultaneously, the expressions of StAR and P450scc were increased when treated with Que +H2O2, compared with the group treated with only H2O2 (P < .05). In conclusion, it is observed that Que could alleviate the H2O2‐induced apoptosis and steroidogenic impairment in goat LGCs, which might be mediated by the Nrf2 pathway.

Quercetin and polycystic ovary syndrome

“…Hydrogen peroxide induced oxidative stress in granulosa cells, and then 20 μM QUR intervention showed that reactive oxygen species (ROS) decreased, NF-E2-related factor 2 (Nrf2) and thioredoxin (Trx) increased, thereby cell damage was repaired. Similar results were seen in granulosa cells of goats and bovines (Khadrawy et al, 2019;Li et al, 2020). In Cadmium-induced chicken granulosa cells, QUR increased X-linked inhibitor of apoptosis protein (XIAP) and decrease caspase-3 and ROS (Jia et al, 2011).…”

“…11 in vitro studies (Jia et al, 2011;Capcarova et al, 2015;Tarko et al, 2018;Sirotkin et al, 2019a;Sirotkin et al, 2019b;Khadrawy et al, 2019;Kolesarova et al, 2019;Rashidi et al, 2019;Li et al, 2020;Yang et al, 2021b;Davoodian et al, 2022) have been included and are shown in Table 1. The cell origin, in vitro model, QUR dosage and biological response have been reviewed.…”

“…QUR not only relieves cell damage by reducing oxidative stress, but also repairs cells by inhibiting apoptosis. QUR has been found to increase B-cell lymphoma-2 (Bcl-2) and decrease Blc-2 associated X protein (Bax) in ovary cells of cattle, gilts, buffalo, goat, chicken and bovine (Jia et al, 2011;Sirotkin et al, 2019b;Li et al, 2020;Yang et al, 2021b;Davoodian et al, 2022). However, it is difficult to prove that the same effect applies to humans because there is rare research on primary granulosa cells from humans.…”

<p>MAGOH/MAGOHB Inhibits the Tumorigenesis of Gastric Cancer via Inactivation of b-RAF/MEK/ERK Signaling</p>

“…It has been confirmed that cleaved caspase 3 and cleaved PARP are important modulators in cell growth. [16][17][18] Huang et al indicated that cleaved caspase 3 is a pro-apoptotic protein. 19 Additionally, cleaved PARP can also be upregulated during the cell apoptosis.…”

Quercetin Alleviates Toxicity Induced by High Levels of Copper in Porcine Follicular Granulosa Cells by Scavenging Reactive Oxygen Species and Improving Mitochondrial Function

“…It is a flavonoid with a wide range of biological activities such as antioxidation, anti-apoptosis, and antiinflammation [53][54][55][56]. It has been reported that QUE protects granulosa cells from damage caused by oxidative stress in pigs [25], goats [57], buffaloes [28], and chickens [58]. Moreover, QUE alleviates the toxic effects of heavy metals such as copper [59], cadmium [60], cisplatin [61], iron [62], and lead [63].…”