Quercetin attenuates cadmium-induced oxidative damage and apoptosis in granulosa cells from chicken ovarian follicles

Jia, Yudong; Lin, Jingkai; Mi, Yuling; Zhang, Caiqiao

doi:10.1016/j.reprotox.2010.12.057

Cited by 86 publications

(59 citation statements)

References 47 publications

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“…Our previous studies have shown that quercetin has effectively inhibited pro-apoptotic member Bax and up-regulated anti-apoptotic member Bcl-XL (Bu et al, 2011;Jia et al, 2011). In the present study, quercetin treatment effectively down-regulated the expression of pro-apoptotic protein Bax and up-regulated the expression of Bcl-XL induced by PNMC.…”

Section: Discussionsupporting

confidence: 64%

Alleviative effect of quercetin on germ cells intoxicated by 3-methyl-4-nitrophenol from diesel exhaust particles

Jia

Lin

et al. 2012

J. Zhejiang Univ. Sci. B

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Abstract:As a component of diesel exhaust particles, 3-methyl-4-nitrophenol (4-nitro-m-cresol, PNMC) is also a metabolite of the insecticide fenitrothion and imposes hazardous effects on human health. In the present study, the alleviative effect of a common antioxidant flavonoid quercetin on mouse germ cells intoxicated by PNMC was investigated. Results showed that a single intraperitoneal injection of PNMC at 100 mg/kg induced severe testicular damage after one week. PNMC-treated mice showed a significant loss of germ cells (approximate 40% loss of round germ cells). PNMC caused an increase of hydroxyl radical and hydrogen peroxide production and lipid peroxidation, as well as a decrease in glutathione level, superoxide dismutase and glutathione peroxidase activities. Furthermore, treatment of PNMC increased expression of the pro-apoptotic protein Bax and decreased expression of the anti-apoptotic protein Bcl-XL in germ cells. In addition, testicular caspase-3 activity was significantly up-regulated and germ cell apoptosis was significantly increased in the PNMC-treated mice. In contrast, combined administration of quercetin at 75 mg/kg significantly attenuated PNMC-induced testicular toxicity. These results indicate that the antioxidant quercetin displays a remarkable protective effect on PNMC-induced oxidative damage in mouse testes and may represent an efficient supplement to attenuate reproductive toxicity by environmental toxicants to ensure healthy sperm production.

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Section: Discussionsupporting

confidence: 64%

Alleviative effect of quercetin on germ cells intoxicated by 3-methyl-4-nitrophenol from diesel exhaust particles

Jia

Lin

et al. 2012

J. Zhejiang Univ. Sci. B

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“…Thus we can speculate that quercetin showed clear antioxidant effect by increasing activity of SOD at the dose of 100 ng/mL, similarly after co-incubation with T-2 toxin. Jia et al [30] stated that quercetin supplementation significantly restored the SOD and GPx activities in granulosa cells from chicken ovarian follicles. Higher activity of GPx in this study after T-2 toxin treatment could suggest the starting involvement of antioxidant defence system.…”

Section: Discussionmentioning

confidence: 99%

Changes in antioxidant status of porcine ovarian granulosa cells after quercetin and T-2 toxin treatment

Capcarová

Petruška

Zbyňovská

et al. 2015

Journal of Environmental Science and Health, Part B

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The aim of this study was to determine the activity of superoxide dismutase (SOD), glutathione peroxidase (GPx), production of reactive oxygen species (ROS), and total antioxidant status (TAS) in porcine ovarian granulosa cells after quercetin and T-2 toxin exposure in vitro. Porcine ovarian granulosa cells were incubated with quercetin and T-2 toxin separately or in mutual combination at the doses of 1 ng/mL, 10 ng/mL, 100 ng/mL, and the control group without any additions for 24 h. In this study T-2 toxin developed stress reaction in porcine ovarian granulosa cells and increased generation of ROS. Quercetin had no effect in elimination of ROS generation induced by T-2 toxin, but was effective in maintaining and increasing of TAS, activities of SOD and GPx in porcine granulosa cells in vitro. These results contribute towards the understanding of cellular stress and its response.

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“…The optical absorbance was measured at 405 nm using a microplate reader (A-5082, Tecan, Austria). The Caspase activities were expressed as the percentage of enzyme activity compared with the control (Jia et al 2011). …”

Section: Caspase Activity Assaysmentioning

confidence: 99%

Induction of apoptosis in HT-29 cells by quercetin through mitochondria-mediated apoptotic pathway

Gao

2013

Animal Cells and Systems

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With increasing use of natural plant-derived components, exploring the anti-proliferative effects of phytochemicals is increasingly gaining importance in designing anticancer drugs. Quercetin, a natural constituent abundantly present in food products, is capable of inducing apoptosis in tumor cells. However, little is known about its biological effect on colon cancer cells and molecular mechanism leading to this event. The aim of this study was to investigate the effect of quercetin on the apoptotic pathway in human colon carcinoma cells (HT-29 cells). The results indicated that quercetin induced suppression of cell viability and apoptosis in HT-29 cells in a dose-dependent manner. This involved characteristic changes in nuclear morphology, activation of caspases-3 and caspases-9, collapse of Mitochondrial membrane potential (DCm), upregulation of pro-apoptotic Bax, and downregulation of antiapoptotic Bcl-2 and Bcl-XL. Quercetin, which exerts anti-proliferative effect though different signaling pathways, is a classic candidate for anti-colon cancer drug design.

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Quercetin attenuates cadmium-induced oxidative damage and apoptosis in granulosa cells from chicken ovarian follicles

Cited by 86 publications

References 47 publications

Alleviative effect of quercetin on germ cells intoxicated by 3-methyl-4-nitrophenol from diesel exhaust particles

Alleviative effect of quercetin on germ cells intoxicated by 3-methyl-4-nitrophenol from diesel exhaust particles

Changes in antioxidant status of porcine ovarian granulosa cells after quercetin and T-2 toxin treatment

Induction of apoptosis in HT-29 cells by quercetin through mitochondria-mediated apoptotic pathway

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