Quercetin effect on the efficiency of ovine oocyte vitrification at GV stage

Editorial: Cryopreservation of mammalian gametes and embryos: implications of oxidative and nitrosative stress and potential role of antioxidants

“…Several exogenous antioxidants have therefore found important usage in cryopreservation and are used at various stages including vitrification, warming, and/or culture media to mitigate oxidative and nitrosative stress, and maintain gamete quality and embryo development ( 6 ). These antioxidants include melatonin ( 24 ), quercetin ( 26 ), vitamin E ( 27 ), resveratrol ( 28 , 29 ), L-carnitine ( 30 ), proline ( 31 ), coenzyme Q10 ( 32 ), and astaxanthin ( 25 ). Exogenous antioxidants however should be used with caution as studies show that beneficial effects are concentration-dependent but too high concentrations have a damaging effect on the gametes and embryos ( 4 ).…”

Oxidative Stress and Oocyte Cryopreservation: Recent Advances in Mitigation Strategies Involving Antioxidants

“…To reduce the cryopreservation-inflicted oxidative damage to oocytes, non-enzymatic antioxidants are usually applied in the vitrification, warming, and/or culture media to mitigate the oxidative stress. Numerous antioxidants including melatonin [ 46 , 88 , 89 ], resveratrol [ 90 , 91 , 92 ], L-carnitine [ 93 , 94 ], quercetin [ 95 ], vitamin E [ 96 , 97 ], astaxanthin [ 98 ], proline [ 99 ], and coenzyme Q10 [ 100 ] have been demonstrated to have beneficial impact on oocyte maturation and development. The results of the previous studies provide encouraging evidence that the oxidative damage in vitrified-warmed oocytes can be reduced/mitigated, and their in vitro developmental potential can be greatly improved.…”

“…It can also activate the Nrf2-ARE antioxidant pathway to improve the activities of downstream related antioxidant enzymes such as SOD and GPX to protect cells from oxidative damage [ 141 , 142 ]. Very recently, it has been reported that addition of 5 × 10 −6 mol/L quercetin was effective in improving the maturation and reducing the apoptosis of vitrified-warmed mouse GV-stage oocytes in vitro, as well as increasing the oocyte cleavage rate and blastocyst formation rate after in vitro fertilization [ 95 ].…”

Oocyte Cryopreservation in Domestic Animals and Humans: Principles, Techniques and Updated Outcomes

“…Information regarding cryopreservation in sheep and goat is relatively limited compared to that in cows. While the outcome in terms of embryo development following conventional slow freezing is poor [72,73], most research has adapted the vitrification technologies from other species aimed at reducing cryoinjuries [72,[74][75][76][77][78][79][80]. However, blastocyst development is still poor, ranging from 0% to 12.5% [75][76][77]81].…”