Quercetin incorporated collagen matrices for dermal wound healing processes in rat

Gomathi, K.; Gopinath, D.; Ahmed, M. Rafiuddin; Jayakumar, R.

doi:10.1016/s0142-9612(03)00059-0

Cited by 146 publications

(82 citation statements)

References 26 publications

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“…These include synthetic polymers, including polyvinyls and polyurethanes, and natural gels, such as collagens, hydrogels and chitins [25][26][27][28]. However, most of these other complex adjuvants focus on providing specific capabilities.…”

Section: Discussionmentioning

confidence: 99%

The effect of multifunctional polymer-based gels on wound healing in full thickness bacteria-contaminated mouse skin wound models

et al. 2007

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We determined whether a two part space-conforming polyethyleneglycol/dopa polymer-based gel promoted healing of contaminated wounds in mice. This silver-catalysed gel was previously developed to be broadly microbiocidal in vitro while being biocompatible with human wound cell functioning. Full-thickness wounds were created on the backs of mice. The wounds were inoculated with 10 4 CFU of each of four common skin wound contaminants, Staphylococcus aureus, Pseudomonas aeruginosa, Acinetobacter baumanii and Clostridium perfringens. The wounds were then treated with our multifunctional polymer-based gel, the commercially-available NewSkin product, or left to heal untreated. The untreated wounds were overtly infected, and presented detectable bacterial loads over the entire 21 day healing period, while the gel and NewSkin groups presented significantly smaller rises in bacterial levels and were cleared of detectable colonies by the third week, with the gel group clearing the bacteria earlier. While all three groups healed their wounds, the polymer-based gel treated group demonstrated signficantly earlier re-epithelialization and dermal maturation (P < 0.05). This was reflected in a quick regain of tensile strength. This accelerated dermal maturation and regain in strength was noted in mice treated with the polymerbased gel when compared to wound treated with the commercially-available Aquacel-Ag dressing (P < 0.05). What distinguishes the polymer-based gel from these other products is that is incorporated within the healing wound. These preclinical studies show that the anti-microbial polymer gel not only supports but also accelerates healing of bacterially contaminated wounds.

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Section: Discussionmentioning

confidence: 99%

The effect of multifunctional polymer-based gels on wound healing in full thickness bacteria-contaminated mouse skin wound models

et al. 2007

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“…The wound-healing activity of the aerial parts of B. ammannioides can be possibly a synergistic effect of its constituents, for instance, b-sitosterol, which was reported to produce rapid re-epithelialization of wounds (Jewo et al, 2009), lupeol, which was reported to have a topical antiinflammatory activity (Saleem, 2009), quercetin and its glycosides, which were reported to increase the wound contraction rate (Gomathi et al, 2003;Manivannan et al, 2014), in addition to other phenolic compounds with free radical scavenging activity. These help in improving wound healing and protect tissues from oxidative damage by reducing the levels of free radicals.…”

Section: Discussionmentioning

confidence: 99%

Antibacterial, antioxidant, and topical anti-inflammatory activities ofBergia ammannioides: A wound-healing plant

Ezzat

Choucry

Kandil

2015

Pharmaceutical Biology

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Context: Despite the traditional use of Bergia ammannioides Henye ex Roth. (Elatinaceae) for the treatment of wounds in India, there is a scarcity of scientific data supporting this use. Objective: The objective of this study is to assess wound-healing potentiality of the plant, to study pharmacological activities that may contribute in eliminating wound complications, and to investigate the biologically active fractions. Material and methods: The ethanolic extract (EtOH) of the aerial parts was fractionated to obtain n-hexane (HxFr), chloroform (ClFr), ethyl acetate (EtFr), and n-butanol (BuOH) fractions. EtOH and its fractions were formulated in strength of 5 and 10% w/w ointment and tested for wound-healing activity using the excision model. The topical anti-inflammatory, in vitro antioxidant, and antibacterial activities were evaluated. HxFr and EtFr were chemically investigated to isolate their constituents. Results: Application of EtOH, HxFr, and EtFr (10% w/w ointments) leads to 71.77, 85.62, and 81.29% healing of the wounds with an increase in the collagen content. HxFr had the strongest anti-inflammatory (64.5% potency relative to Voltaren Õ ) and antibacterial activity (MIC ¼ 104 mg/ml against Staphylococcus aureus), while EtFr showed the strongest antioxidant activity against DPPH, ABTS + , and super oxide radical with an IC 50 value of 10.25 ± 0.01, 66.09 ± 0.76, and 167.33 ± 0.91 mg/ml, respectively. b-Sitosterol, lupeol, cyclolaudenol, and cycloartenol were isolated from HxFr. Quercetin, ellagic acid, kaempferol-3-O-a-L-rhamnoside, and quercetin-3-O-a-L-rhamnoside were isolated from EtFr. Discussion and conclusion: Our study presents scientific evidence for the efficacy of B. ammannioides in enhancing wound healing, and the first isolation of cyclolaudenol and cycloartenol from Bergia.

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“…Among materials used, the naturally derived polymers including collagen [7][8][9] and chitosan [10][11][12] have received special attention because of their biological and chemical similarities with natural tissues. Collagen dressings have been used to address a variety of surgical problems including www.scienceasia.org burns and wounds.…”

Section: Introductionmentioning

confidence: 99%

Efficacy evaluation of the fibroblast-seeded collagen/chitosan scaffold on application in skin tissue engineering

Intaraprasit¹,

Faikrua²,

Sittichokechaiwut³

et al. 2012

ScienceAsia

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There remains a need for dressings which aid the healing of chronically refractory wounds. To this end, we formulated and tested a collagen/chitosan scaffold supporting basic fibroblast growth factor (bFGF) producing cells. Collagen was blended with chitosan derived from crab shell (in ratio 7:3 of 3% weight of total polymer), crosslinked with glutaraldehyde, and then cast into a film. Tensile strength and elongation at break values of the scaffold was 8.5 ± 0.1 MPa and 2.4 ± 0.6%, respectively. The swelling degree was 77 ± 4%, and the weight remaining after collagenase degradation for 1 month was 30.5 ± 6.3%. Our scaffolds showed bio-compatibility because they encouraged attachment and proliferation of spindle-shaped human dermal fibroblasts (HDFs) which secreted surface-bound bFGF. Next, the fibroblast-seeded scaffold was prepared by seeding HDFs on the collagen/chitosan scaffold (5 × 10 5 cells/cm 2 ) and further cultured for 7 days.These fibroblast-seeded scaffolds were used to dress full depth wounds in domestic pigs. The wounds with fibroblastseeded scaffolds showed reduced swelling at day 7 and at all time points (7, 14, and 21 days), and re-epithelialization was faster than controls. Thus at day 21 of treatment, wounds treated with the fibroblast-seeded scaffold provided the highest percent re-epithelialization (69.7 ± 9.6%), which was higher than untreated (49.9 ± 6.3%) and cell-free scaffold treatment (54.3 ± 6.9%). The wound tissue of the fibroblast-seeded scaffold treated group showed regular arrangement of host collagens and the rete ridge-like structure at the epidermal layer was also found at day 21. The results indicate the potential of the fibroblast-seeded scaffold for application in skin tissue engineering.

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Quercetin incorporated collagen matrices for dermal wound healing processes in rat

Cited by 146 publications

References 26 publications

The effect of multifunctional polymer-based gels on wound healing in full thickness bacteria-contaminated mouse skin wound models

The effect of multifunctional polymer-based gels on wound healing in full thickness bacteria-contaminated mouse skin wound models

Antibacterial, antioxidant, and topical anti-inflammatory activities ofBergia ammannioides: A wound-healing plant

Efficacy evaluation of the fibroblast-seeded collagen/chitosan scaffold on application in skin tissue engineering

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