Quercetin simultaneously induces G<sub>0</sub>/G<sub>1</sub>-phase arrest and caspase-mediated crosstalk between apoptosis and autophagy in human leukemia HL-60 cells

Chang, Junn Liang; Chow, Jyh Ming; Chang, Jer‐Hwa; Wen, Yu‐Ching; Lin, Yung Wei; Yang, Shun Fa; Lee, Wei Jiunn; Chien, Ming Hsien

doi:10.1002/tox.22408

Cited by 30 publications

(23 citation statements)

References 42 publications

(47 reference statements)

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“…For example, quercetin induced cytoprotective autophagy in HL-60 cells, and inhibition of autophagy may be a novel strategy to enhance the anticancer activity of quercetin in AML. (23) Moreover, quercetin also induces protective autophagy in ovarian cancer, (24) lymphoma (25) and glioma. (26) However, high levels of autophagy might lead to cell death in cancers.…”

Section: Discussionmentioning

confidence: 99%

Quercetin induced NUPR1-dependent autophagic cell death by disturbing reactive oxygen species homeostasis in osteosarcoma cells

Zeng

Ouyang

et al. 2020

J. Clin. Biochem. Nutr.

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Osteosarcoma is a primary bone aggressive cancer, affecting adolescents worldwide. Quercetin (a natural polyphenolic com pound) is a polyphenolic flavonoid compound found in a variety of plants. It has been demonstrated to exert cytostatic activity against a variety of human cancer, including the human osteo sarcoma. However, its efficacy in the treatment of osteosarcoma and the underlying antitumor mechanism has not been fully elucidated yet. In this study, we exposed MG 63 cells to different concentrations of quercetin (50, 100 and 200 µM) for 24 h. Here, we show that quercetin increased autophagic flux in the MG 63 cells, as evidenced by the upregulation of LC3B II/LC3B I and downregulation of P62/SQSTM1. Moreover, the autophagy inhibitor Bafilomycin A1 or genetic blocking autophagy with ATG5 knock down decreased quercetin induced cell death, indicating quercetin triggered autophagic cell death in MG 63 cells. Specifically, quercetin increased NUPR1 expression and activated of NUPR1 reporter activity, which contributed to the expression of autophagy related genes and subsequent initiated autophagic cell death in osteo sarcoma cells. Importantly, the increased expression NUPR1 were tightly related to the disturbance of reactive oxygen species (ROS) homeostasis, which could be prevented by inhibiting intracellular ROS with NAC. Finally, NAC also abolished quercetin induced autophagic cell death in vivo. Taken together, these data demon strate that quercetin induces osteosarcoma cell death via inducing excessive autophagy, which is mediated through the ROS NUPR1 pathway. Quercetin application may be a promising and practical strategy for osteosarcoma treatment in clinical practice.

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Section: Discussionmentioning

confidence: 99%

Quercetin induced NUPR1-dependent autophagic cell death by disturbing reactive oxygen species homeostasis in osteosarcoma cells

Zeng

Ouyang

et al. 2020

J. Clin. Biochem. Nutr.

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“…To determine the effect of β‐Mangostin on human HCC cell viability, cells were treated with various concentrations of β‐mangostin (0, 2.5, 5, 7.5, and 10 μM) for 24 and 48 hours, and were subjected to the MTT assay. The absorbance of blue formazan crystals was measured at 570 nm using an enzyme‐linked immunosorbent assay plate reader.…”

Section: Methodsmentioning

confidence: 99%

β‐mangostin suppresses human hepatocellular carcinoma cell invasion through inhibition of MMP‐2 and MMP‐9 expression and activating the ERK and JNK pathways

Huang

Teng

et al. 2017

Environmental Toxicology

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β-mangostin is a dietary xanthone that has been reported to have the anticancer properties in some human cancer cell types. However, the antimetastatic effect and molecular mechanism of β-mangostin action in human hepatocellular carcinoma (HCC) cells remain unknown. In this study, we found that β-mangostin did not induce cytotoxicity in human HCC cells (SK-Hep-1, Huh-7 and HA22T/VGH cells). β-mangostin could inhibit migration and invasion of human HCC cells. Meanwhile, β-mangostin significantly decreased the protein activities and expression of matrix metalloproteinase (MMP)-2 and MMP-9 via increasing the activation of MEK1/2, ERK1/2, MEK4 and JNK1/2 signaling pathways. Furthermore, using specific inhibitor for ERK1/2 (PD98059) and JNK1/2 (JNKII) significantly restored the expression of MMP-2/-9 and invasion by β-mangostin treatment in Huh-7 cells. In addition, β-mangostin effectively restored the protein levels and transcription activity of MMP-2 and MMP-9 in siERK or siJNK-transfected Huh-7 cells, concomitantly with promotion on cell migration and invasion. Taken together, these findings are the first to demonstrate the antimetastatic activity of β-mangostin against human HCC cells, which may act as a promising therapeutic agent for the treatment of HCC.

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“…This inhibition of autophagy can be an effective strategy to enhance the anticancer activity of quercetin in AML (J.‐L. Chang et al, ).…”

Section: Anticancer Perspectives Of Quercetinmentioning

confidence: 99%

Anticancer potential of quercetin: A comprehensive review

Rauf

Imran

Khan

et al. 2018

Phytotherapy Research

503

276

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Diet plays a key role to maintaining healthy life. Many natural products present in our diet, such as flavonoids, can prevent the progression of cancer. Quercetin, a distinctive bioactive flavonoid, is a dietary component that has attracted the attention of dietitians and medicinal chemists due to its numerous health-promoting effects. It is an outstanding antioxidant that has a well-documented role in reducing different human cancers. Quercetin exhibits direct proapoptotic effects on tumor cells and thus can inhibit the progress of numerous human cancers. The anticancer effect of quercetin has been documented in numerous in vitro and in vivo studies that involved several cell lines and animal models. On the other hand, the high toxic effect of quercetin against cancer cells is accompanied with little or no side effects or harm to normal cells. Accordingly, this review presents an overview of recent developments on the use of quercetin against different types of cancer along with mechanisms of action. In addition, the present review summarizes the literature pertaining to quercetin as an anticancer agent and provides an assessment of the potential utilization of this natural compound as a complimentary or alternative medicine for preventing and treating cancer.

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Quercetin simultaneously induces G₀/G₁-phase arrest and caspase-mediated crosstalk between apoptosis and autophagy in human leukemia HL-60 cells

Cited by 30 publications

References 42 publications

Quercetin induced NUPR1-dependent autophagic cell death by disturbing reactive oxygen species homeostasis in osteosarcoma cells

Quercetin induced NUPR1-dependent autophagic cell death by disturbing reactive oxygen species homeostasis in osteosarcoma cells

β‐mangostin suppresses human hepatocellular carcinoma cell invasion through inhibition of MMP‐2 and MMP‐9 expression and activating the ERK and JNK pathways

Anticancer potential of quercetin: A comprehensive review

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Quercetin simultaneously induces G0/G1-phase arrest and caspase-mediated crosstalk between apoptosis and autophagy in human leukemia HL-60 cells

Cited by 30 publications

References 42 publications

Quercetin induced NUPR1-dependent autophagic cell death by disturbing reactive oxygen species homeostasis in osteosarcoma cells

Quercetin induced NUPR1-dependent autophagic cell death by disturbing reactive oxygen species homeostasis in osteosarcoma cells

β‐mangostin suppresses human hepatocellular carcinoma cell invasion through inhibition of MMP‐2 and MMP‐9 expression and activating the ERK and JNK pathways

Anticancer potential of quercetin: A comprehensive review

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Quercetin simultaneously induces G₀/G₁-phase arrest and caspase-mediated crosstalk between apoptosis and autophagy in human leukemia HL-60 cells