Quercus ilex Phyllosphere Microbiome Environmental-Driven Structure and Composition Shifts in a Mediterranean Contex

Postiglione, Alessia; Prigioniero, Antonello; Zuzolo, Daniela; Tartaglia, Maria Carmela; Scarano, Pierpaolo; Maisto, Maria; Ranauda, Maria Antonietta; Sciarrillo, Rosaria; Thijs, Sofie; Vangronsveld, Jaco; Guarino, Carmine

doi:10.3390/plants11243528

Cited by 7 publications

(3 citation statements)

References 87 publications

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“…The variation in leaf characteristics and environmental factors between young and old leaves, along with the influence of host plant species, play crucial roles in shaping leaf-associated communities and account for the observed differences (S ̌igutovaé t al., 2023). Overall, our study provides confirmation of significant seasonal fluctuations in the composition of phyllosphere epiphytic bacteria and fungi (Jumpponen and Jones, 2010;Gomes et al, 2018;Postiglione et al, 2022).…”

Section: Epiphytic Bacterial and Fungal Community Construction In Phy...supporting

confidence: 73%

Seasonal dynamics of phyllosphere epiphytic microbial communities of medicinal plants in farmland environment

He,

Zhang,

et al. 2024

Front. Plant Sci.

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IntroductionThe phyllosphere of plants is inhabited by various microorganisms, which play a crucial role in plant physiological metabolism. Currently, there is limited research on the dynamic effects of species and seasons on plant phyllosphere microbial community diversity and microbial interactions.MethodsIn this study, high-throughput sequencing technology was used to sequence the leaf surface parasitic microorganisms of five medicinal plants (Bupleurum chinense, Atractylodes lancea, Salvia miltiorrhiza, Astragalus membranaceus, and Lonicera japonica).ResultsThe results showed that bacteria and fungi clustered into 3,898 and 1,572 operational taxonomic units (OTUs), respectively. Compared to host species, seasons had a more significant impact on the a diversity of bacteria and fungi. The heterogeneity of phyllosphere microbial communities was greater in winter compared to summer. Key species analysis at the OTU level and Spearman correlation analysis demonstrated significant preferences in microbial interactions under plant and seasonal backgrounds. The network connections between bacterial and fungal communities significantly increased during seasonal transitions compared to connections with plants.DiscussionThis study enhances our understanding of the composition and ecological roles of plant-associated microbial communities in small-scale agricultural environments. Additionally, it provides valuable insights for assessing the biodiversity of medicinal plants.

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Section: Epiphytic Bacterial and Fungal Community Construction In Phy...supporting

confidence: 73%

Seasonal dynamics of phyllosphere epiphytic microbial communities of medicinal plants in farmland environment

He,

Zhang,

et al. 2024

Front. Plant Sci.

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“…Notably, the multi-functionality of phyllosphere microorganisms, including bacteria and fungi, showed no notable correlation with climatic conditions ( Wei et al, 2022 ). The findings underscore the complex interplay of environmental factors, plant morphology, and microbial diversity, emphasizing their profound implications for the stability and functionality of phyllosphere microorganism communities ( Stapleton and Simmons, 2006 ; Postiglione et al, 2022 ).…”

Section: Discussionmentioning

confidence: 92%

Unveiling the diversity, composition, and dynamics of phyllosphere microbial communities in Alhagi sparsifolia across desert basins and seasons in Xinjiang, China

Zhang,

Du,

Zhang

et al. 2024

Front. Microbiol.

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Phyllosphere microbes residing on plant leaf surfaces for maintaining plant health have gained increasing recognition. However, in desert ecosystems, knowledge about the variety, composition, and coexistence patterns of microbial communities in the phyllosphere remains limited. This study, conducted across three basins (Turpan-TLF, Tarim-CL, and Dzungaria-MSW) and three seasons (spring, summer, and autumn) in Xinjiang, China, aimed to explore the diversity and composition of microbial communities in the phyllosphere, encompassing both bacteria and fungi in Alhagi sparsifolia. We also investigated the co-occurrence patterns, influencing factors, and underlying mechanisms driving these dynamics. Results indicate that phyllosphere bacteria exhibited lower diversity indices (ACE, Shannon, Simpson, Fisher phylogenetic diversity, and Richness) in spring compared to summer and autumn, while the Goods Coverage Index (GCI) was higher in spring. Conversely, diversity indices and GCI of phyllosphere fungi showed an opposite trend. Interestingly, the lowest level of multi-functionality and niche width in phyllosphere bacteria occurred in spring, while the highest level was observed in phyllosphere fungi. Furthermore, the study revealed that no significant differences in multi-functionality were found among the regions (CL, MSW, and TLF). Network analysis highlighted that during spring, phyllosphere bacteria exhibited the lowest number of nodes, edges, and average degree, while phyllosphere fungi had the highest. Surprisingly, the multi-functionality of both phyllosphere bacteria and fungi showed no significant correlation with climatic and environmental factors but displayed a significant association with the morphological characteristics and physicochemical properties of leaves. Structural Equation Model indicated that the morphological characteristics of leaves significantly influenced the multi-functionality of phyllosphere bacteria and fungi. However, the indirect and total effects of climate on multi-functionality were greater than the effects of physicochemical properties and morphological characteristics of leaves. These findings offer new insights into leaf phyllosphere microbial community structure, laying a theoretical foundation for vegetation restoration and rational plant resource utilization in desert ecosystems.

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“…For each of the ten samples, 4000 FASTQ reads were obtained; sequence data were deposited in the NCBI under BioProject PRJNA1095819 (biosamples SAMN40733751–SAMN40733760). The sequences were imported into GAIA metagenomics cloud-based software (v2.02, Sequentia Biotech, Barcelona, Spain) [ 28 , 29 ] for subsequent bioinformatics analyses.…”

Section: Methodsmentioning

confidence: 99%

MinION Sequencing of Fungi in Sub-Saharan African Air and a Novel LAMP Assay for Rapid Detection of the Tropical Phytopathogenic Genus Lasiodiplodia

King,

Canning,

West

2024

Pathogens

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To date, there have been no DNA-based metabarcoding studies into airborne fungi in tropical Sub-Saharan Africa. In this initial study, 10 air samples were collected onto Vaseline-coated acrylic rods mounted on drones flown at heights of 15–50 meters above ground for 10–15 min at three sites in Ghana. Purified DNA was extracted from air samples, the internal transcribed spacer (ITS) region was amplified using fungal-specific primers, and MinION third-generation amplicon sequencing was undertaken with downstream bioinformatics analyses utilizing GAIA cloud-based software (at genus taxonomic level). Principal coordinate analyses based on Bray–Curtis beta diversity dissimilarity values found no clear evidence for the structuring of fungal air communities, nor were there significant differences in alpha diversity, based on geographic location (east vs. central Ghana), underlying vegetation type (cocoa vs. non-cocoa), or height above ground level (15–23 m vs. 25–50 m), and despite the short flight times (10–15 min), ~90 operational taxonomic units (OTUs) were identified in each sample. In Ghanaian air, fungal assemblages were skewed at the phylum taxonomic level towards the ascomycetes (53.7%) as opposed to basidiomycetes (24.6%); at the class level, the Dothideomycetes were predominant (29.8%) followed by the Agaricomycetes (21.8%). The most common fungal genus in Ghanaian air was cosmopolitan and globally ubiquitous Cladosporium (9.9% of reads). Interestingly, many fungal genera containing economically important phytopathogens of tropical crops were also identified in Ghanaian air, including Corynespora, Fusarium, and Lasiodiplodia. Consequently, a novel loop-mediated isothermal amplification (LAMP) assay, based on translation elongation factor-1α sequences, was developed and tested for rapid, sensitive, and specific detection of the fungal phytopathogenic genus Lasiodiplodia. Potential applications for improved tropical disease management are considered.

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Quercus ilex Phyllosphere Microbiome Environmental-Driven Structure and Composition Shifts in a Mediterranean Contex

Cited by 7 publications

References 87 publications

Seasonal dynamics of phyllosphere epiphytic microbial communities of medicinal plants in farmland environment

Seasonal dynamics of phyllosphere epiphytic microbial communities of medicinal plants in farmland environment

Unveiling the diversity, composition, and dynamics of phyllosphere microbial communities in Alhagi sparsifolia across desert basins and seasons in Xinjiang, China

MinION Sequencing of Fungi in Sub-Saharan African Air and a Novel LAMP Assay for Rapid Detection of the Tropical Phytopathogenic Genus Lasiodiplodia

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