“…S3, ESI †). These results demonstrated that although NR, as an electron shuttle, was worse than RF probably because the more positive E 00 value of RF (À0.208 V) 7 than NR (À0.325 V) 41 thermodynamically favored the microbial metabolism, 43 the difference in current density between NDH II and MR-1 in NR (2.6-fold) was larger than that in RF (2.0-fold) because the former bypassed the electron transfer process via the protein conduit. The cyclic voltammetry (CV) measurements of bioanodes were conducted under conditions as follows: when the anolyte was replenished (without any detectable RF), 22 h after anolyte replenishment (containing 1.09 AE 0.08 mM RF, n = 3) and 200 mM additional RF was added.…”