Quinoa proteins ( Chenopodium quinoa Willd.) fractionated by ultrafiltration using ceramic membranes: The role of pH on physicochemical and conformational properties

Navarro-Lisboa, Rosa; Herrera, Camila; Zúñiga, Rommy N.; Enrione, Javier; Guzmán, Fanny; Matiacevich, Silvia; Astudillo‐Castro, Carolina

doi:10.1016/j.fbp.2016.11.005

Cited by 39 publications

(15 citation statements)

References 57 publications

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“…The major proteins in quinoa seeds are albumins and globulins, accounting for 35% and 37%, respectively [11]. Recently, much attention has been given to the nutritional value and functional properties of quinoa protein [14,15], and bioactive peptides such as DPP-IV (dipeptidyl-peptidase IV) inhibitory peptides and antioxidant peptides have been identified from quinoa protein isolate and globulin [16][17][18][19]. However, scant information is available about the antioxidant and ACE-inhibitory activities of quinoa albumin or its hydrolysates.…”

Section: Introductionmentioning

confidence: 99%

Isolation of Novel ACE-Inhibitory and Antioxidant Peptides from Quinoa Bran Albumin Assisted with an In Silico Approach: Characterization, In Vivo Antihypertension, and Molecular Docking

et al. 2019

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Albumin is the major fraction of quinoa protein that is characterized as having high nutritional value. However, until now, scant information is available on the bioactivity of quinoa albumin or its hydrolysates. To promote its usage, we extracted albumin in this study from quinoa bran assisted with cellulase and hemicellulose, and hydrolyzed it by alcalase and trypsin to produce bioactive peptides. The hydrolysates (QBAH) were purified by gel filtration and reversed-phase high-performance liquid chromatography (RP-HPLC), followed by identification using liquid chromatography-mass spectrometry (LC-MS/MS). Furthermore, based on in silico analysis, one angiotensin-I converting enzyme (ACE)-inhibitory and antioxidant peptide, RGQVIYVL (946.6 Da), and two antioxidant peptides, ASPKPSSA (743.8 Da), and QFLLAGR (803.5 Da), from QBAH were synthesized. RGQVIYVL showed a high ACE-inhibitory activity (IC 50 = 38.16 µM) with competitive mode of inhibition, and showed significant antihypertensive effect in spontaneously hypertensive rats at a concentration of 100-150 mg/kg body weight (bw). Molecular docking simulation showed that it could interact with the active ACE site via hydrogen bonds with high binding power. Moreover, RGQVIYVL, ASPKPSSA, and QFLLAGR all demonstrated high ·OH scavenging activity (IC 50 = 61.69-117.46 µM), ABTS + scavenging activity (58.29-74.28%) and Fe 2+ chelating ability (32.54-82.48% at 0.5 mg/mL). They could also retain activity after gastrointestinal enzyme digestion. These results indicate that quinoa albumin is a potential source of bioactive peptides possessing antioxidant and ACE-inhibitory activities.

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Section: Introductionmentioning

confidence: 99%

Isolation of Novel ACE-Inhibitory and Antioxidant Peptides from Quinoa Bran Albumin Assisted with an In Silico Approach: Characterization, In Vivo Antihypertension, and Molecular Docking

et al. 2019

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“…), a pseudocereal that is native to South America, has attracted much research interest lately, not only for its nutritional profile but also for its adaptability to different growing conditions [1,2]. Quinoa has a relatively high protein content (14-16%) [3], and presents a wider amino acid spectrum than cereals and legumes [4], with higher lysine (5.1-6.4%) and methionine (0.4-1.0%) contents [5]. This makes quinoa complementary to cereals and legumes [6].…”

Section: Introductionmentioning

confidence: 99%

The Influence of Starch and Fibre on In Vitro Protein Digestibility of Dry Fractionated Quinoa Seed (Riobamba Variety)

et al. 2018

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The in vitro gastric digestibility of the quinoa variety Riobamba was investigated, especially the influence of the quinoa matrix. Dry-fractionated quinoa protein concentrate, which is just milled and sieved, was much better digestible than the same concentrate that was reconstituted from wet fractionated quinoa protein isolate, quinoa starch isolate, and quinoa fibre isolate. In the reconstituted concentrate, the presence of quinoa starch and fibre next to quinoa protein reduces its in vitro gastric digestibility significantly. However, the effect of starch is partially counteracted if the fibre is also present. While the effects of starch and fibre separately can be understood from the decrease of the accessibility for pepsin to hydrolyse proteins, due to the hydrated starch and fibre, we suspect that the synergistic effect of starch and fibre may be due to a relative reduction of the hydration of starch due to the presence of the also strongly hydrating fibre. We concluded that the presence of starch and fibre decreases the protein in vitro gastric digestibility. Therefore, the presence of fibre partially countered the decreased of the protein digestibility of starch. Heating of the matrices to 120°C generally resulted in much lower digestion rates, due to extensive aggregation of the protein.

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“…The leaves and seeds of this plant are rich food sources of proteins, with a broad spectrum of amino acids, and various types of minerals and vitamins (Encina-Zelada et al, 2017). Recent data demonstrate that the seeds of quinoa consist of 14% proteins (Navarro-Lisboa et al, 2017), but the protein content can be increased to 20%, which has been reported for improved varieties (Giménez et al, 2016). Therefore, the high agronomic features and high nutritive values of quinoa prompted scientists and agronomists to focus their research efforts on this valuable plant (Betalleluz Pallardel et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Optimizing sterilization conditions and growth regulator effects on in vitro shoot regeneration through direct organogenesis in Chenopodium quinoa

Hesami

Naderi

Yoosefzadeh-Najafabadi

2018

bta

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Chenopodium quinoa Willd. is known as a multipurpose crop that could be employed as an alternative on unsuitable lands with limited water resources such as arid and semi-arid regions. However, the biggest problem in cultivating this plant is contamination by viruses that are carried by quinoa seeds and cause diseases. Therefore, in vitro propagation methods could be applied to produce virus-free plants. The capacity of sodium hypochlorite to sterilize seeds and the regeneration potential of cotyledonary node segments were studied on Murashige and Skoog (MS) medium consisting of various concentrations of cytokinins and auxins either alone or in combinations. Although no contamination was observed after sterilization with 20% sodium hypochlorite and 10 and 15 min of immersion; with 25% sodium hypochlorite and 5, 10, and 15 min of immersion, the highest seed germination (100%) was obtained after sterilization with 20% sodium hypochlorite for 5 minutes. MS medium supplemented with 2.0 mg/l 6-benzylaminopurine (BAP) had the highest shoot induction frequency (93.33%) with an average of 4.96 shoots per node explant. This study is the first report of an appropriate and effective in vitro regeneration protocol for Chenopodium quinoa via direct organogenesis through cotyledonary node explants derived from axenic-seedlings, which can be effectively employed for the genetic manipulations and exploitation of active biomolecules of this valuable plant. This protocol can also increase the speed of quinoa breeding programs.

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Quinoa proteins ( Chenopodium quinoa Willd.) fractionated by ultrafiltration using ceramic membranes: The role of pH on physicochemical and conformational properties

Cited by 39 publications

References 57 publications

Isolation of Novel ACE-Inhibitory and Antioxidant Peptides from Quinoa Bran Albumin Assisted with an In Silico Approach: Characterization, In Vivo Antihypertension, and Molecular Docking

Isolation of Novel ACE-Inhibitory and Antioxidant Peptides from Quinoa Bran Albumin Assisted with an In Silico Approach: Characterization, In Vivo Antihypertension, and Molecular Docking

The Influence of Starch and Fibre on In Vitro Protein Digestibility of Dry Fractionated Quinoa Seed (Riobamba Variety)

Optimizing sterilization conditions and growth regulator effects on in vitro shoot regeneration through direct organogenesis in Chenopodium quinoa

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