Quinoid pigments of echinodermata V. Pigments of the sea urchinStrongy locentrotus dröebachiensis

Kol′tsova, E. A.; Denisenko, V. A.; Maksimov, O. B.

doi:10.1007/bf00565235

Cited by 13 publications

(27 citation statements)

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“…Normally, HCl-aqueous solution is used to dissolve the calcareous skeleton to produce the free pigments (Amarowicz et al, 1994;Kol'tsova et al, 1978;Mischenko et al, 2005;Utkina et al, 1976). However, we found that the dissolution with HClaqueous solution generated a lot of bubbles which adversely influenced the subsequent extraction.…”

Section: Extraction Of Phnq Pigments By Macroporous Resinmentioning

confidence: 88%

“…From then on, there are about thirty quinonoid pigments have been isolated from sea urchin of different species (Amarowicz, Synowiecki, & Shahidi, 1994;Anderson, Mathieson, & Thomson, 1969;Kol'tsova, Denisenko, & Maksimov, 1978;Mischenko et al, 2005;Utkina, Shchedrin, & Maksimov, 1976;Yakubovskaya, Pokhilo, Mishchenko, & Anufriev, 2007). Those quinonoid pigments almost all have the polyhydroxylated 1,4-naphoquinone (PHNQ) structure with ethyl, acetyl, methoxy and amino as substitutes (Amarowicz et al, 1994;Anderson et al, 1969;Kol'tsova et al, 1978;Mischenko et al, 2005;Utkina et al, 1976;Yakubovskaya et al, 2007). In recent years, the PHNQ pigments were found to possess excellent antimicrobial, antialgal, cardioprotective and antioxidant activities (Mischenko et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

“…Usually, the shells and spines are dissolved in HCl-aqueous solution, and then the PHNQ pigments are extracted by organic solvents such as diethyl ether and chloroform (Amarowicz et al, 1994;Kol'tsova et al, 1978;Mischenko et al, 2005;Utkina et al, 1976). Those organic solvents are toxic and volatile, indicating the operation is harmful to the operators and the environment.…”

Section: Introductionmentioning

confidence: 99%

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Extraction and antioxidant property of polyhydroxylated naphthoquinone pigments from spines of purple sea urchin Strongylocentrotus nudus

et al. 2011

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Section: Extraction Of Phnq Pigments By Macroporous Resinmentioning

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Extraction and antioxidant property of polyhydroxylated naphthoquinone pigments from spines of purple sea urchin Strongylocentrotus nudus

et al. 2011

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“…Echinochrome (2,3,5,7,8-pentahydroxynaphthoquinone), a quinoid pigment of sea-urchins, is the active ingredient of 1% histochrome for injections [3,13]. The preparation is a dark red liquid.…”

Section: Resultsmentioning

confidence: 99%

“…Russian-manufactured chloroform for anesthesia and distilled ethanol were used in experiments. Echinochrome (2,3,5,7,, a quinoid pigment of sea-urchins, is the active ingredient of 1% histochrome for injections [3,13]. The preparation is a dark red liquid.…”

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Determination of plasma concentration of the cardioprotective preparation histochrome

et al. 1996

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A method for measuring the plasma concentration of histochrome is described. Echinochrome, a naturally occurring polyhydroxynaphthoquinone with antioxidant and cardioprotective activities, is the active ingredient of histochrome. Plasma histochrome concentration is measured in patients with acute myocardial infarction. This method can be used for pharmacokinetic analysis. Key Words: free-radical pathology; antioxidants, polyhydroxynaphthoquinones; histochrome; pharmacokineticsAntioxidants have been widely used in the treatment of various diseases [12]. Histochrome is a new antioxidant drug [1,4,5] exhibiting cardioprotective activity in experimental reperfusion-induced iSchemia [5,6,9]. Clinical trials showed that in combination with thrombolytics this drug effectively prevents the reperfusion-induced damage to the myocardium after acute infarction. Monitoring of histochrome concentration in biological fluids is necessary for the optimization of therapeutic scheme and evaluation of the drug activity.Our objective was to develop a method for determination of plasma concentration of histochrome after its injection. MATERIALS AND METHODSHealthy donor plasma was used as a model. Histochrome was added to it to a final concentration of 1-40 ~tg/ml.Plasma prepared from heparinized blood of healthy donors and patients with acute myocardial infarction Cardiology-Research Center, Russian Academy of Medical Sciences, Moscow; Pacific Institute of Bioorganic Chemistry, Russian Academy of Sciences, Vladivostok was stored in liquid nitrogen. The specimens were thawed immediately before experiments, and chylomicrons were sedimented by centrifugation (80,000g, 30 min, 6~ a Beckman centrifuge) [9]. All other procedures were performed at room temperature. Plasma concentration of histochrome was measured in a Shimadzu UV-260 spectrophotometer.The preparation, histochrome for injections (1% water solution), was produced at the Experimential Plant of Biomedical Preparations (Cardiology Research Center) using materials and technology from the Pacific Institute of Bioorganic Chemistry (Russian Academy of Sciences). Russian-manufactured chloroform for anesthesia and distilled ethanol were used in experiments. Echinochrome (2,3,5,7,, a quinoid pigment of sea-urchins, is the active ingredient of 1% histochrome for injections [3,13]. The preparation is a dark red liquid. After its intravenous injection in a dose of 1-2 mg/kg body weight, biologic fluids were stained red. RESULTSIndividual variations of the plasma pigment levels and overlapping of histochrome and plasma pigment

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Diversity of Polyhydroxynaphthoquinone Pigments in North Pacific Sea Urchins

Васильева

Mishchenko

Fedoreyev

2017

Chemistry & Biodiversity

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Using high-performance liquid chromatography with diode-array detection and mass spectrometry (HPLC-DAD/MS) we investigated the composition of polyhydroxynaphthoquinone (PHNQ) pigments from sea urchins Strongylocentrotus pallidus, St. polyacanthus, St. droebachiensis, Brisaster latifrons and Echinarachnius parma, collected in the Sea of Okhotsk and the Bering Sea. Identification of PHNQ pigments from sea urchins St. polyacanthus, B. latifrons, and E. parma was performed for the first time. Among the usual PHNQ pigments, mono- and dimethoxy derivatives of spinochrome E, not previously found in other sea urchins, were discovered in St. polyacanthus and St. droebachiensis. In St. droebachiensis, two monomethoxy derivatives of echinochrome A were detected, isolated previously from only tropical sea urchins. It was found that the composition and total content of pigments of St. droebachiensis depends on the collection area of the sea urchins and its depth and varies from 88 to 331 μg/g of dry shells. Sea urchins St. pallidus, B. latifrons and E. parma had average values for PHNQ pigment content, approximately 30 μg/g, and St. polyacanthus had a low PHNQ content, 13 μg/g.

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Quinoid pigments of echinodermata V. Pigments of the sea urchinStrongy locentrotus dröebachiensis

Cited by 13 publications

References 3 publications

Extraction and antioxidant property of polyhydroxylated naphthoquinone pigments from spines of purple sea urchin Strongylocentrotus nudus

Extraction and antioxidant property of polyhydroxylated naphthoquinone pigments from spines of purple sea urchin Strongylocentrotus nudus

Determination of plasma concentration of the cardioprotective preparation histochrome

Diversity of Polyhydroxynaphthoquinone Pigments in North Pacific Sea Urchins

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