Quinolone, fluoroquinolone and trimethoprim/sulfamethoxazole resistance in relation to virulence determinants and phylogenetic background among uropathogenic Escherichia coli

Moreno, Eva; Prats, Guillem; Sabaté, Montserrat; Pérez, Teresa; Johnson, James R.; Andreu, Antònia

doi:10.1093/jac/dki468

Cited by 138 publications

(124 citation statements)

References 27 publications

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“…The phylogenetic group D includes pathogenic strains such as E. coli O157:H7, which is highly virulent and can cause diarrhea, hemolytic uremic syndrome and hemorrhagic colitis (Parry and Palmer, 2000). Previous studies have demonstrated that group B2 E. coli strains are less resistant to antibiotics (Laroche et al, 2009;Moreno et al, 2006) and it is assumed that they tend to carry fewer integrons than the others phylogenetic E. coli groups (Skurnik et al 2005). …”

Section: Usa 4 % Of the Tetracycline Resistant Isolates Were Positivementioning

confidence: 99%

Antibiotic resistance and virulence genes in coliform water isolates

Stange

Sidhu

Tiehm

et al. 2016

International Journal of Hygiene and Environmental Health

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Please cite this article as: Stange, C., Sidhu, J.P.S., Tiehm, A., Toze, S., Antibiotic resistance and virulence genes in coliform water isolates.International Journal of Hygiene and Environmental Health http://dx.doi.org/10. 1016/j.ijheh.2016.07.015 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. 1Antibiotic resistance and virulence genes in coliform water isolates Graphical abstract2 ABSTRACTWidespread fecal pollution of surface water may present a major health risk and a significant pathway for dissemination of antibiotic resistance bacteria. The River Rhine is one of the longest and most important rivers in Europe and an important raw water source for drinking water production. A total of 100 coliform isolates obtained from River Rhine (Germany) were examined for their susceptibility to seven antimicrobial agents. Resistances against amoxicillin, trimethoprim/sulfamethoxazole and tetracycline were detected in 48 %, 11 % and 9 % of isolates respectively. The antibiotic resistance could be traced back to the resistance genes blaTEM, blaSHV, ampC, sul1, sul2, dfrA1, tet(A) and tet (B). Whereby, the ampC gene represents a special case, because its presence is not inevitably linked to a phenotypic antibiotic resistance. Multiple antibiotics resistance was often accompanied by the occurrence of class 1 or 2 integrons. E. coli isolates belonging to phylogenetic groups A and B1(commensal) were more predominant (57 %) compared to B2 and D groups (43 %) which are known to carry virulent genes. Additionally, six E. coli virulence genes were also detected. However, the prevalence of virulence genes in the E. coli isolates was low (not exceeding 4.3 % per gene) and no diarrheagenic E. coli pathotypes were detected. This study demonstrates that surface water is an important reservoir of ARGs for a number of antibiotic classes such as sulfonamide, trimethoprim, betalactam-antibiotics and tetracycline. The occurrence of antibiotic resistance in coliform bacteria isolated from River Rhine provides evidence for the need to develop management strategies to limit the spread of antibiotic resistant bacteria in aquatic environment.

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Section: Usa 4 % Of the Tetracycline Resistant Isolates Were Positivementioning

confidence: 99%

Antibiotic resistance and virulence genes in coliform water isolates

Stange

Sidhu

Tiehm

et al. 2016

International Journal of Hygiene and Environmental Health

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“…Similar findings have occurred in earlier studies in other parts of the world where low prevalence of VGs were detected among FQ resistant CEC isolates (Horcajada et al, 2005;Huang et al, 2009;Kawamura-Sato et al, 2010). These isolates may have been opportunistic pathogens that acquired FQ resistance (Drews et al, 2005;Vila et al, 2002) or they may be CEC isolates that lost their VGs after they developed mutations in the QRDR (Horcajada et al, 2005;Moreno et al;Sawma-Aouad et al, 2009). Furthermore, the virulence profile of the AmpC harbouring E. coli isolates also varied.…”

Section: Discussionmentioning

confidence: 99%

“…Similar to other studies, isolates with a > 93% similarity in their ERIC profile were assumed to be closely related (a clonal group) (Moreno et al, 2006;Sabate et al, 2008). A cluster was defined as a group of isolates that shared ≥ 80% similarity in their ERIC-PCR profile patterns.…”

Section: Enterobacterial Repetitive Intergenic Consensus (Eric)-pcrmentioning

confidence: 96%

“…(Horcajada et al, 2005;Huang et al, 2009;Kawamura-Sato et al, 2010). These isolates may have been opportunistic pathogens that acquired FQ resistance (Drews et al, 2005;Vila et al, 2002) or they may be CEC isolates that lost their VGs after they developed mutations in the quinolone resistance-determining region (QRDR) (Horcajada et al, 2005;Moreno et al, 2006;Sawma-Aouad et al, 2009 This might be part of a much larger study, which may explore the distribution of AmpC β-lactamase genes in avian E. coli isolates at different geographical locations and how similar Australian isolates are to overseas strains.…”

Section: Investigating the Prevalence Of Carriage Of Avian Pathogenicmentioning

confidence: 99%

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Studies on avian pathogenic Escherichia coli in commercial broiler chicken in South East Queensland

Awawdeh¹,

Turni²,

Henning³

et al.

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Avian pathogenic Escherichia coli (APEC) is the causative agent of avian colibacillosis, a localised or systemic infection resulting in clinical diseases such as colisepticemia, chronic respiratory disease and swollen-head syndrome. Globally, avian colibacillosis is the leading cause of morbidity and mortality in poultry, and it has been associated with massive economic losses and welfare problems.This organism is of public health significance as APEC is communicable to humans. The diagnosis of avian colibacillosis relies on clinical signs, typical pathological lesions and culture of E. coli from affected tissue(s). Antimicrobial therapy is often used both for treatment and control. Previous overseas studies have characterised APEC and identified virulence genes (VGs) that can be used as molecular markers for the identification of APEC. Little is known about APEC in broiler chickens in Australia.The aim of this thesis was to gain a better understanding of the epidemiology of APEC in Australian broiler flocks and how factors including presence of VGs and phylogenetic group can improve the identification of this pathotype in Australia. Firstly, three faecal DNA extraction methods were evaluated. Faeces were collected from healthy chickens and chickens with colibacillosis from commercial broiler farms in South East Queensland (SEQ). The extracted DNA was screened by a pentaplex-PCR for five APEC-associated VGs (iroN, iutA, iss, hlyF and ompT). DNA extracted from E. coli isolates cultured from the cloaca and organs of the birds were screened using the same PCR.Repeated bead beating plus column elution was the preferred DNA extraction method, as it yielded good PCR quality and adequate quantity DNA. However, identifying APEC by direct detection of the five VGs from the faecal material was not feasible as all of these genes were also detected in all of the birds. However, the VGs were more commonly detected in E. coli isolates cultured from birds with colibacillosis.A cross-sectional study was performed to estimate APEC farm-level prevalence in healthy broiler chickens in SEQ and to identify potential risk factors associated with the carriage of APEC. At the farm-level, all of the 40 farms sampled were positive for APEC, that is at least one bird per farm carried APEC, while the within-farm prevalence was 63% (95% Confidence Interval: 55.8, 70.2).Higher APEC within-farm bird-level prevalence was significantly associated with the usage of well water as a source of drinking water, failure to disinfect the water line after each flock, farm visitors not showering before entering the shed, distances greater than 20 metres between the car park and the poultry shed and the presence of wild birds within 50 metres of the shed. Chlorinating the drinking water combined with automatic water filtration reduced within-farm bird-level APEC prevalence. Page | 3Therefore, based on the results concluded from the multivariable model, improving biosecurity and water treatments might reduce APEC prevalence, decrease the risk of co...

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“…However, association of the susceptible isolates with the phylogenetic group B2 and that of the resistant isolates with non-B2 group have also been reported [21][22][23][24]. The controversy existent among these reports may be interpreted as being the result of the number of samples analyzed, geographical variations, or various clinical sources.…”

Section: Determination Of Virulence Genesmentioning

confidence: 99%

Molecular Characterization of Uropathogenic Escherichia coli : Nalidixic Acid and Ciprofloxacin Resistance, Virulent Factors and Phylogenetic Background

Basu

Mukherjee

Hazra

et al. 2013

JCDR

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Background and Objective: A proficient pathogen should be virulent, resistant to antibiotics, and epidemic. However, the interplay between resistance and virulence is poorly under stood. Perhaps, the most commonly accepted view is that resistance to quinolones is linked to a loss of virulence factors. However, the low virulent phylogenetic groups may be more prone to acquire resistance to quinolones. The aim of this study was to identify and characterise the Nalidixic Acid (NA) and ciprofloxacin (CIP) resistant uropathogenic Escherichia coli (UPEC) isolates with respect to virulence and phylogenetic background, from hospital settings in Kolkata, an eastern region in India. Research based on these bacterial populations will help in understanding the molecular mechanisms underlying the association between resistance and virulence, that in turn, may help in managing the future disseminations of UTIs in their entirety.

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Quinolone, fluoroquinolone and trimethoprim/sulfamethoxazole resistance in relation to virulence determinants and phylogenetic background among uropathogenic Escherichia coli

Cited by 138 publications

References 27 publications

Antibiotic resistance and virulence genes in coliform water isolates

Antibiotic resistance and virulence genes in coliform water isolates

Studies on avian pathogenic Escherichia coli in commercial broiler chicken in South East Queensland

Molecular Characterization of Uropathogenic Escherichia coli : Nalidixic Acid and Ciprofloxacin Resistance, Virulent Factors and Phylogenetic Background

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