Quorum Quenching Bacillus sonorensis Isolated from Soya Sauce Fermentation Brine

Abstract

An N -acylhomoserine lactone (AHL)-degrading bacterial strain, L62, was isolated from a sample of fermentation brine of Chinese soya sauce by using rich medium agar supplemented with soya sauce (10% v/v). L62, a rod-shaped Gram positive bacterium with amylolytic activity, was phylogentically related to Bacillus sonorensis by 16S ribosomal DNA and rpoB sequence analyses. B. sonorensis L62 efficiently degraded N -3-oxohexanoyl homoserine lactone and N -octanoylhomoserine lactone. However, the aiiA homologue, encoding an autoinducer inactivation enzyme catalyzing the degradation of AHLs, was not detected in L62, suggesting the presence of a different AHL-degrading gene in L62. To the best of our knowledge, this is the first report of AHL-degrading B. sonorensis from soya sauce liquid state fermentation.

Quorum Quenching Enzymes and Their Application in Degrading Signal Molecules to Block Quorum Sensing-Dependent Infection

“…Recently, B. amyloliquefaciens , B. subtilis , B. mycoides and B. marcorestinctum were shown to have AHL-degrading activities, whereas AHL-degrading activity has not yet been reported for B. pseudomycoides [11,12,25,26]. AHL-degrading B. sonorensis L62 was isolated from a sample of the fermentation brine of Chinese soy sauce [27], which efficiently degraded N -(3-oxohexanoyl)- homoserine lactone (3-oxo-C6-HSL) and N -octanoyl-homoserine lactone (C8-HSL). The aiiA homologue was not detected in B. sonorensis L62, suggesting the presence of a different AHL-degrading gene in the strain L62.…”

Piper nigrum, Piper betle and Gnetum gnemon- Natural Food Sourcewith Anti-Quorum Sensing Properties

“…Anti-QS effects can be achieved by enzymatic approaches or using natural products [13]. Many quorum quenching bacteria producing AHL degradation enzymes have been isolated and studied [14–17]. Recent studies have demonstrated that QS antagonist compounds can be found in higher plants such as peas, vanilla, raspberry, Melicope lunu-ankenda , clove, and Myristica cinnamomea [18–23].…”

Long Chain N-acyl Homoserine Lactone Production by Enterobacter sp. Isolated from Human Tongue Surfaces

“…When the concentration of these AHLs reaches the threshold level, the AHL-luxR complex will regulate a set of genes which occur in a population density-dependent manner, leading to population driven changes in several functions including virulence determinants, antibiotic production, bioluminescence, and biofilm formation [10–13]. QS bacteria have been isolated from various sources and habitats, including the human body [14–18]. …”