Quorum quenching bacteria can be used to inhibit the biofouling of reverse osmosis membranes

Oh, Hyun‐Suk; Tan, Chuan Hao; Low, Jiun Hui; Rzechowicz, Miles; Siddiqui, Muhammad Faisal; Winters, Harvey; Kjelleberg, Staffan; Fane, Anthony G.; Rice, Scott A.

doi:10.1016/j.watres.2017.01.028

Cited by 74 publications

(51 citation statements)

References 52 publications

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“…The impact of QQ bacteria on biofilm formation by P. aeruginosa PAO1 was studied using a recently described segregated culture bioassay ( Oh et al, 2017 ). In this assay, QQ bacteria are physically separated from PAO1 by using a semipermeable membrane (Transwell polycarbonate membrane cell inserts; Corning, NY, United States).…”

Section: Methodsmentioning

confidence: 99%

“…In aquaculture industry, QQ has shown positive results in the disruption of bacterial infections ( Cao et al, 2012 ; Romero et al, 2014 ; Vinoj et al, 2014 ; Torres et al, 2016 ). Recently, QQ was tested for its ability to mitigate the biofouling of reverse osmosis membranes used in seawater desalination ( Oh et al, 2017 ). QQ also has other potential applications such as control of biofouling on the hulls of shipping vessels and fishnets and bio-corrosion of oil production wells.…”

Section: Introductionmentioning

confidence: 99%

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Quorum-Quenching Bacteria Isolated From Red Sea Sediments Reduce Biofilm Formation by Pseudomonas aeruginosa

Rehman

Leiknes

2018

Front. Microbiol.

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Quorum sensing (QS) is the process by which bacteria communicate with each other through small signaling molecules such as N-acylhomoserine lactones (AHLs). Certain bacteria can degrade AHL molecules by a process called quorum quenching (QQ); therefore, QQ can be used to control bacterial infections and biofilm formation. In this study, we aimed to identify new species of bacteria with QQ activity. Red Sea sediments were collected either from the close vicinity of seagrass or from areas with no vegetation. We isolated 72 bacterial strains, which were tested for their ability to degrade/inactivate AHL molecules. Chromobacterium violaceum CV026-based bioassay was used for the initial screening of isolates with QQ activity. QQ activity was further quantified using high-performance liquid chromatography-tandem mass spectrometry. We found that these isolates could degrade AHL molecules of different acyl chain lengths as well as modifications. 16S-rRNA sequencing of positive QQ isolates showed that they belonged to three different genera. Specifically, two isolates belonged to the genus Erythrobacter; four, Labrenzia; and one, Bacterioplanes. The genome of one representative isolate from each genus was sequenced, and potential QQ enzymes, namely, lactonases and acylases, were identified. The ability of these isolates to degrade the 3OXOC12-AHLs produced by Pseudomonas aeruginosa PAO1 and hence inhibit biofilm formation was investigated. Our results showed that the isolate VG12 (genus Labrenzia) is better than other isolates at controlling biofilm formation by PAO1 and degradation of different AHL molecules. Time-course experiments to study AHL degradation showed that VG1 (genus Erythrobacter) could degrade AHLs faster than other isolates. Thus, QQ bacteria or enzymes can be used in combination with an antibacterial to overcome antibiotic resistance.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Quorum-Quenching Bacteria Isolated From Red Sea Sediments Reduce Biofilm Formation by Pseudomonas aeruginosa

Rehman

Leiknes

2018

Front. Microbiol.

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“…Genetic modifications could also be used to construct bacterial strains with specific QQ activity. That may be obtained by inserting genes (e.g., aiiO gene encodes AHL degrading enzyme-AHL lactonase) into competent cells' genomes as was described by Oh et al (2017). The strains modified in that way gain QQ activity.…”

Section: Measurement Of Ai Productionmentioning

confidence: 99%

Prevention of biofilm formation by quorum quenching

Paluch

Rewak‐Soroczyńska

Jędrusik

et al. 2020

Appl Microbiol Biotechnol

303

151

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Quorum sensing (QS) is a mechanism that enables microbial communication. It is based on the constant secretion of signaling molecules to the environment. The main role of QS is the regulation of vital processes in the cell such as virulence factor production or biofilm formation. Due to still growing bacterial resistance to antibiotics that have been overused, it is necessary to search for alternative antimicrobial therapies. One of them is quorum quenching (QQ) that disrupts microbial communication. QQ-driving molecules can decrease or even completely inhibit the production of virulence factors (including biofilm formation). There are few QQ strategies that comprise the use of the structural analogues of QS receptor autoinductors (AI). They may be found in nature or be designed and synthesized via chemical engineering. Many of the characterized QQ molecules are enzymes with the ability to degrade signaling molecules. They can also impede cellular signaling cascades. There are different techniques used for testing QS/QQ, including chromatography-mass spectroscopy, bioluminescence, chemiluminescence, fluorescence, electrochemistry, and colorimetry. They all enable qualitative and quantitative measurements of QS/QQ molecules. This article gathers the information about the mechanisms of QS and QQ, and their effect on microbial biofilm formation. Basic methods used to study QS/QQ, as well as the medical and biotechnological applications of QQ, are also described. Basis research methods are also described as well as medical and biotechnological application.

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“…Artificial seawater (26.29 g/L NaCl, 0.74 g/L KCl, 1.32 g/L CaCl 2 dehydrated, 6.09 g/L MgCl 2 ·7H 2 O, 1.92 g/L MgSO 4 , pH 7.8) was used as feed solution. Nutrient broth (HiMedia, Mumbai, India) was added to the feed solution to obtain a nutrient concentration of 24 mg/L as described previously (Oh et al, 2017 ). Feed bottle was inoculated with P. aeruginosa culture at a final concentration of 5 × 10 7 CFU/mL and this feed was replaced every 3 d. Biofilm was established on each membrane for 6 d with a recording of the transmembrane pressure (TMP) every 8 h.…”

Section: Methodsmentioning

confidence: 99%

Bacteriophage Infectivity Against Pseudomonas aeruginosa in Saline Conditions

et al. 2018

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Pseudomonas aeruginosa is a ubiquitous member of marine biofilm, and reduces thiosulfate to produce toxic hydrogen sulfide gas. In this study, lytic bacteriophages were isolated and applied to inhibit the growth of P. aeruginosa in planktonic mode at different temperature, pH, and salinity. Bacteriophages showed optimal infectivity at a multiplicity of infection of 10 in saline conditions, and demonstrated lytic abilities over all tested temperature (25, 30, 37, and 45°C) and pH 6–9. Planktonic P. aeruginosa exhibited significantly longer lag phase and lower specific growth rates upon exposure to bacteriophages. Bacteriophages were subsequently applied to P. aeruginosa-enriched biofilm and were determined to lower the relative abundance of Pseudomonas-related taxa from 0.17 to 5.58% in controls to 0.01–0.61% in treated microbial communities. The relative abundance of Alphaproteobacteria, Pseudoalteromonas, and Planococcaceae decreased, possibly due to the phage-induced disruption of the biofilm matrix. Lastly, when applied to mitigate biofouling of ultrafiltration membranes, bacteriophages were determined to reduce the transmembrane pressure increase by 18% when utilized alone, and by 49% when used in combination with citric acid. The combined treatment was more effective compared with the citric acid treatment alone, which reported ca. 30% transmembrane pressure reduction. Collectively, the findings demonstrated that bacteriophages can be used as a biocidal agent to mitigate undesirable P. aeruginosa-associated problems in seawater applications.

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Quorum quenching bacteria can be used to inhibit the biofouling of reverse osmosis membranes

Cited by 74 publications

References 52 publications

Quorum-Quenching Bacteria Isolated From Red Sea Sediments Reduce Biofilm Formation by Pseudomonas aeruginosa

Quorum-Quenching Bacteria Isolated From Red Sea Sediments Reduce Biofilm Formation by Pseudomonas aeruginosa

Prevention of biofilm formation by quorum quenching

Bacteriophage Infectivity Against Pseudomonas aeruginosa in Saline Conditions

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