Quorum Sensing Activity in Pandoraea pnomenusa RB38

Ee, Robson; Lim, Yan-Lue; Kin, Lin-Xin; Yin, Wai‐Fong; Chan, Kok‐Gan

doi:10.3390/s140610177

Cited by 5 publications

(7 citation statements)

References 32 publications

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“…However, isolate identification performed in a previous study using 16S rDNA sequencing and Matrix-assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) identified strain RB38 as P . pnomenusa ( Ee et al, 2014b ).…”

Section: Resultsmentioning

confidence: 99%

“…We previously reported the QS activity of P. pnomenusa RB38 ( Ee et al, 2014b ). In this study, we identified the putative luxI and luxR 1 homologs from the annotated genome.…”

Section: Resultsmentioning

confidence: 99%

“…at the genomic level is relatively superficial where majority of the literatures focuses firstly on usage of genotypic data to facilitate in accurate genus- and species-level identification ( Coenye et al, 2001 ; Coenye & LiPuma, 2002 ) and secondly on their biotechnological potential ( Schneider, Queenan & Bauernfeind, 2006 ; Jiang et al, 2009 ; Colbert et al, 2013 ; Ee et al, 2015 ). Furthermore, to date, inclusive of our recent report on the QS activity in P. pnomenusa RB38 ( Ee et al, 2014b ), there are only three publications about the documentation of the QS activity in Pandoraea spp. ( Han-Jen, Wai-Fong & Kok-Gan, 2013 ; Chan et al, 2015 ).…”

Section: Introductionmentioning

confidence: 99%

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Complete genome sequencing ofPandoraea pnomenusaRB38 and Molecular Characterization of ItsN-acyl homoserine lactone synthase geneppnI

Lim

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et al. 2015

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In this study, we sequenced the genome of Pandoraea pnomenusa RB38 using Pacific Biosciences RSII (PacBio) Single Molecule Real Time (SMRT) sequencing technology. A pair of cognate luxI/R homologs was identified where the luxI homolog, ppnI, was found adjacent to a luxR homolog, ppnR1. An additional orphan luxR homolog, ppnR2, was also discovered. Multiple sequence alignment and phylogenetic analysis revealed that ppnI is an N-acyl homoserine lactone (AHL) synthase gene that is distinct from those of the nearest phylogenetic neighbor viz. Burkholderia spp. High resolution tandem mass spectrometry (LC-MS/MS) analysis showed that Escherichia coli BL21 harboring ppnI produced a similar AHL profile (N-octanoylhomoserine lactone, C8-HSL) as P. pnomenusa RB38, the wild-type donor strain, confirming that PpnI directed the synthesis of AHL in P. pnomenusa RB38. To our knowledge, this is the first documentation of the luxI/R homologs of the genus Pandoraea.

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Section: Resultsmentioning

confidence: 99%

“…We previously reported the QS activity of P. pnomenusa RB38 ( Ee et al, 2014b ). In this study, we identified the putative luxI and luxR 1 homologs from the annotated genome.…”

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Complete genome sequencing ofPandoraea pnomenusaRB38 and Molecular Characterization of ItsN-acyl homoserine lactone synthase geneppnI

Lim

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et al. 2015

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“…Members of genus Pandoraea have been reported with QS activity and are able to communicate via the production of AHLs (Ee et al, 2014). In this study, we investigated (1) if AHL-mediated QS is a common activity employed by all type strains of Pandoraea species and (2) the distribution of QS genes in Pandoraea genus.…”

Section: Introductionmentioning

confidence: 99%

In silico Analysis Reveals Distribution of Quorum Sensing Genes and Consistent Presence of LuxR Solos in the Pandoraea Species

Chua

See-Too

et al. 2019

Front. Microbiol.

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The most common quorum sensing (QS) system in Gram-negative bacteria consists of signaling molecules called N- acyl-homoserine lactones (AHLs), which are synthesized by an enzyme AHL synthase (LuxI) and detected by a transcriptional regulator (LuxR) that are usually located in close proximity. However, many recent studies have also evidenced the presence of LuxR solos that are LuxR-related proteins in Proteobacteria that are devoid of a cognate LuxI AHL synthase. Pandoraea species are opportunistic pathogens frequently isolated from sputum specimens of cystic fibrosis (CF) patients. We have previously shown that P. pnomenusa strains possess QS activity. In this study, we examined the presence of QS activity in all type strains of Pandoraea species and acquired their complete genome sequences for holistic bioinformatics analyses of QS-related genes. Only four out of nine type strains ( P. pnomenusa , P. sputorum , P. oxalativorans, and P. vervacti ) showed QS activity, and C8-HSL was the only AHL detected. A total of 10 canonical luxI s with adjacent luxR s were predicted by bioinformatics from the complete genomes of aforementioned species and publicly available Pandoraea genomes. No orphan luxI was identified in any of the genomes. However, genes for two LuxR solos (LuxR2 and LuxR3 solos) were identified in all Pandoraea genomes (except two draft genomes with one LuxR solo gene), and P. thiooxydans was the only species that harbored no QS-related activity and genes. Except the canonical LuxR genes, LuxIs and LuxR solos of Pandoraea species were distantly related to the other well-characterized QS genes based on phylogenetic clustering. LuxR2 and LuxR3 solos might represent two novel evolutionary branches of LuxR system as they were found exclusively only in the genus. As a few luxR solos were located in close proximity with prophage sequence regions in the genomes, we thus postulated that these luxR solos could be transmitted into genus Pandoraea by transduction process mediated by bacteriophage. The bioinformatics approach developed in this study forms the basis for further characterization of closely related species. Overall, our findings improve the current understanding of QS in Pandoraea species, which is a potential pharmacological target in battling Pandoraea infections in CF patients.

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“…In the fourth cycle, 50 µl of enriched KGm medium was plated on KGm agar, and colonies of bacteria with distinctive morphology were isolated and streaked until a single colony was obtained. Strain RB-25 T was selected for further analysis as it activated a CV026 biosensor and it was routinely cultivated at 28 C in Luria-Bertani (LB) media (Ee et al, 2014b;Lim et al, 2014;McClean et al, 1997). Colonies of strain RB-25 T were slightly raised with very pale white pigmentation on LB medium after 24 h of incubation.…”

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confidence: 99%

Chania multitudinisentens gen. nov., sp. nov., an N-acyl-homoserine-lactone-producing bacterium in the family Enterobacteriaceae isolated from landfill site soil

Madhaiyan

et al. 2016

International Journal of Systematic and Evolutionary Microbiology

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Phylogenetic and taxonomic characterization was performed for bacterium RB-25 T , which was isolated from a soil sample collected in a former municipal landfill site in Puchong, Malaysia. Growth occurred at 20-37 C at pH 5-8 but not in the presence of 9 % (w/v) NaCl or higher. The principal fatty acids were C 16:0 , C 18:1 !7c and summed feature 3 (C 16:1 !7c and/or iso-C 15:0 2-OH). Ubiquinone-8 was the only isoprenoid quinone detected. Polar lipid analysis revealed the presence of phospholipid, phosphoaminolipid, phosphatidylethanolamine, phosphatidylglycerol and one unidentified aminolipid. DNA G+C content was 50.9 mol% phylogenetic analysis based on 16S rRNA gene sequence showed that strain RB-25 T formed a distinct lineage within the family Enterobacteriaceae of the class Gammaproteobacteria. It exhibited a low level of 16S rRNA gene sequence similarity with its phylogenetic neighbours Pantoea rwandensis LMG 26275 T (96.6 %), Rahnella aquatilis CIP 78.65 T (96.5 %), Pectobacterium betavasculorum ATCC 43762 T (96.4 %), Pantoea rodasii LMG 26273 T (96.3 %), Gibbsiella dentisursi NUM 1720 T (96.3 %) and Serratia glossinae C1 T (96.2 %). Multilocus sequence analyses based on fusA, pyrG, rplB, rpoB and sucA sequences showed a clear distinction of strain RB-25 T from the most closely related genera. Isolate RB-25 T could also be distinguished from members of these genera by a combination of the DNA G+C content, respiratory quinone system, fatty acid profile, polar lipid composition and other phenotypic features. Strain RB-25 T represents a novel species of a new genus, for which the name Chania multitudinisentens gen. nov., sp. nov. is proposed. The type strain is RB-25 T (=DSM 28811 T =LMG 28304 T ).

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Quorum Sensing Activity in Pandoraea pnomenusa RB38

Cited by 5 publications

References 32 publications

Complete genome sequencing ofPandoraea pnomenusaRB38 and Molecular Characterization of ItsN-acyl homoserine lactone synthase geneppnI

Complete genome sequencing ofPandoraea pnomenusaRB38 and Molecular Characterization of ItsN-acyl homoserine lactone synthase geneppnI

In silico Analysis Reveals Distribution of Quorum Sensing Genes and Consistent Presence of LuxR Solos in the Pandoraea Species

Chania multitudinisentens gen. nov., sp. nov., an N-acyl-homoserine-lactone-producing bacterium in the family Enterobacteriaceae isolated from landfill site soil

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