Quorum-sensing gene <i>luxS</i> regulates flagella expression and Shiga-like toxin production in F18ab <i>Escherichia coli</i>

Yang, Yang; Zhu, Guoqiang; Hou, Huayan; Zhu, Jun; Yao, Fenghua; Zhang, Xinjun; Zhu, Xiaofang; Hardwidge, Philip R.; Zhu, Guoqiang

doi:10.1139/cjm-2014-0178

Cited by 25 publications

(23 citation statements)

References 52 publications

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“…coli (66). Both flagellar and universal stress response genes are known to be important virulence factors for pathogenic bacteria (68, 69). For example, mice infected with isogenic uspA mutants of S.…”

Section: Discussionmentioning

confidence: 99%

Evidence of Naturalized Stress-Tolerant Strains of Escherichia coli in Municipal Wastewater Treatment Plants

Zhi

Banting

et al. 2016

Appl Environ Microbiol

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Escherichia coli has been proposed to have two habitats—the intestines of mammals/birds and the nonhost environment. Our goal was to assess whether certain strains of E. coli have evolved toward adaptation and survival in wastewater. Raw sewage samples from different treatment plants were subjected to chlorine stress, and ∼59% of the surviving E. coli strains were found to contain a genetic insertion element (IS30) located within the uspC-flhDC intergenic region. The positional location of the IS30 element was not observed across a library of 845 E. coli isolates collected from various animal hosts or within GenBank or whole-genome reference databases for human and animal E. coli isolates (n = 1,177). Phylogenetics clustered the IS30 element-containing wastewater E. coli isolates into a distinct clade, and biomarker analysis revealed that these wastewater isolates contained a single nucleotide polymorphism (SNP) biomarker pattern that was specific for wastewater. These isolates belonged to phylogroup A, possessed generalized stress response (RpoS) activity, and carried the locus of heat resistance, features likely relevant to nonhost environmental survival. Isolates were screened for 28 virulence genes but carried only the fimH marker. Our data suggest that wastewater contains a naturalized resident population of E. coli. We developed an endpoint PCR targeting the IS30 element within the uspC-flhDC intergenic region, and all raw sewage samples (n = 21) were positive for this marker. Conversely, the prevalence of this marker in E. coli-positive surface and groundwater samples was low (≤5%). This simple PCR assay may represent a convenient microbial source-tracking tool for identification of water samples affected by municipal wastewater.IMPORTANCE The results of this study demonstrate that some strains of E. coli appear to have evolved to become naturalized populations in the wastewater environment and possess a number of stress-related genetic elements likely important for survival in this nonhost environment. The presence of non-host-adapted strains in wastewater challenges our understanding of using E. coli as a microbial indicator of wastewater treatment performance, suggesting that the E. coli strains present in human and animal feces may be very different from those found in treated wastewater.

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Section: Discussionmentioning

confidence: 99%

Evidence of Naturalized Stress-Tolerant Strains of Escherichia coli in Municipal Wastewater Treatment Plants

Zhi

Banting

et al. 2016

Appl Environ Microbiol

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“…AI-2 is encoded by the luxS gene and has diverse effects on different bacteria. For example, the luxS deletion mutant of E. coli reduced the adhesion of flagellum, expression of Stx2e toxin, and production of bacterial surface outer membrane proteins (Yang et al, 2014 ). However, a recent work demonstrated that the inactivation of the luxS gene of S. aureus increased biofilm formation and higher polysaccharide intercellular adhesion (PIA) production (Ma et al, 2017 ).…”

Section: The Effects Of Metabolites From Gut Microbiota On Pathogensmentioning

confidence: 99%

Effects of Metabolites Derived From Gut Microbiota and Hosts on Pathogens

Quan

Jiang

et al. 2018

Front. Cell. Infect. Microbiol.

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126

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Intestinal metabolites participate in various physiological processes, including energy metabolism, cell-to-cell communication, and host immunity. These metabolites mainly originate from gut microbiota and hosts. Although many host metabolites are dominant in intestines, such as free fatty acids, amino acids and vitamins, the metabolites derived from gut microbiota are also essential for intestinal homeostasis. In addition, some metabolites are only generated and released by gut microbiota, such as bacteriocins, short-chain fatty acids, and quorum-sensing autoinducers. In this review, we summarize recent studies regarding the crosstalk between pathogens and metabolites from different sources, including the influence on bacterial development and the activation/inhibition of immune responses of hosts. All of these functions would affect the colonization of and infection by pathogens. This review provides clear ideas and directions for further exploring the regulatory mechanisms and effects of metabolites on pathogens.

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“…Quorum sensing governs the control and regulation of all the metabolic activities of bacterial cell. The effects of these molecular signalling networks have been thoroughly studied and documented for a number of phenotypes, including biofi lm formation, toxin production, motility and exopolysaccharide formation (Yang et al 2014 ;Wang et al 2014 ).…”

Section: Glow While Speakingmentioning

confidence: 99%

Tryptophan Metabolites: A Microbial Perspective

Engin

2015

Molecular and Integrative Toxicology

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The discovery of the regulation of tryptophan biosynthetic pathway by means of a repressible operon has been recognised as a milestone in genetics. This long multistep pathway is energetically so expensive that numerous allosteric control loops exist in addition to tight genetic regulation. That's why essential amino acid tryptophan is a valuable product for food industry and animal breeding. Traditionally, vitamin auxotrophs of soil microorganisms have been used for amino acid manufacturing. However, in the age of synthetic biology, metabolic engineering has recently become the method of choice to construct the producer strains.Additionally, in contrast to mammalian cells, bacterial cells are able to produce tryptophan starting at central metabolic intermediates from pentose phosphate pathway and glycolysis. This metabolic divergence provides an excellent target for the development of novel antimicrobials which interfere with the biosynthesis of tryptophan.Tryptophan metabolism makes at least two contributions to microbial quorumsensing pathways, which may have implications in antimicrobial chemotherapy. The autoinducers of renowned Pseudomonas quinolone signalling system originate either from kynurenine or shikimate pathways. Fluorinated 4-quinolone derivative antimicrobial drugs exhibit antipathogenic effects at subinhibitory concentration, presumably by interfering with Pseudomonas quinolone signalling. Another recently recognised bacterial signal molecule is indole, a degradation product of tryptophan. Unconventional stationary phase signal molecule indole is unique, as no receptor/response regulator protein has been identifi ed to date. Instead, the effects of indole have been attributed to its physicochemical interaction with the cell membrane.

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Quorum-sensing gene luxS regulates flagella expression and Shiga-like toxin production in F18ab Escherichia coli

Cited by 25 publications

References 52 publications

Evidence of Naturalized Stress-Tolerant Strains of Escherichia coli in Municipal Wastewater Treatment Plants

Evidence of Naturalized Stress-Tolerant Strains of Escherichia coli in Municipal Wastewater Treatment Plants

Effects of Metabolites Derived From Gut Microbiota and Hosts on Pathogens

Tryptophan Metabolites: A Microbial Perspective

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