R2205 Prevalence of enteropathogenic and shiga toxin-producing Escherichia coli among children with and without diarrhoea in Iran

Alikhani, M Y; Aslani, M M; Mirsalehian, Akbar; Fatollahzadeh, B.

doi:10.1016/s0924-8579(07)72044-7

Cited by 19 publications

(22 citation statements)

References 27 publications

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“…Enteropathogenic E. coli (EPEC) is commonly transmitted via the fecal-oral route in a poor hygienic environment 27 . Results of studies carried out in areas with different health and socio-demographic characteristics showed that strains of EPEC were the major cause of diarrhea in developing countries 24 . Results of a study conducted in southern Iran showed that most of the cases of acute diarrhea were due to sero-groups of EPEC including O127, O86, O126, O142, O55, O119, O128, and sero-groups O127 and O86 were more frequently found 22 .…”

Section: Discussionmentioning

confidence: 99%

Antibiogram of E. coli serotypes isolated from children aged under five with acute diarrhea in Bahir Dar town

et al. 2015

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Background: Diarrheal disease and its complications remain a major cause of morbidity and mortality in children. The prevalence and antibiogram of E. coli as causative agents of diarrhea vary from region to region, and even within countries in the same geographical area. Objectives: To determine the serotype and antimicrobial susceptibility of E. coli in children under-five years of age. Methods: A cross-sectional study was conducted among 422 children with diarrhea from December 2011 to February 2012. Identification of E. coli and antimicrobial susceptibility testing were done following standard procedures. Results: The overall isolation rate of E. coli was 48.3%. Poly 2 sero-groups, poly 3 sero-groups, poly 4 sero-groups and E. coli O157:H7 accounted for 80 (39.2%), 40 (19.6%), 25 (12.3%), and 59 (28.9%) of the isolates, respectively. Poly 2 sero-groups, constituting isolates belonging to enteropathogenic E. coli were the most commonly isolated serotypes. E. coli exhibited high levels of antimicrobial resistance to ampicillin (86.8%), tetracycline (76%) and cotrimoxazole (76%). Low levels of resistance to ciprofloxacin (6.9%) and norfloxacin (9.3%) were documented. Conclusion: High prevalence of diarrheagenic E. coli compounded by alarming antimicrobial resistances is a serious public health problem. Regular determination of antibiogram and public education are recommended.

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Section: Discussionmentioning

confidence: 99%

Antibiogram of E. coli serotypes isolated from children aged under five with acute diarrhea in Bahir Dar town

et al. 2015

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“…Colibacillosis, associated with E. coli, occurs in all species of new born farms animals and it is a major cause of economic loss in this age and it cause diarrhea particularly in calves less than 30 days of age (Rodostitis et al, 2007). It is a common cause of gastroenteritis and it accounts for 30% of the total number of diarrhea pathogenic in some regions of the world (Alikhani et al, 2007). Resistance to the anti-microbial agents is recognized as a major global public health problem, infectious diseases are for approximately one-half of all cases of death in different beings (Iwu et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

In vitro and in vivo Antibacterial Activity of Ethanolic Extract of Sweet Basil (Ocimum basilicum L.) Leaves against Escherichia coli in Experimentally Infected Rats

Ibrahim¹

2015

Adv. Anim. Vet. Sci.

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| The present study was carried out to investigate the antibacterial activity of ethanol extract of Ocimum basilicum against infectious diarrhea caused by E. coli. Two experiments were performed in this present study; the first one is in vitro antibacterial activity of Ocimum basilicum ethanolic extract. The second experiment included study in vivo antibacterial activity of the extract after inducing diarrhea with oral pathogenic E. coli in five rats groups (eight rats of each). The results of the in vitro antibacterial study showed that E. coli was more sensitive to ethanolic extract of O.B in comparison with Gentamicin and Amikacin and it was resistant to the metronidazole, but E. coli was more sensitive to Trimethoprim/Sulfamethoxazole. Two doses of O.B extract 100, 200 mg/kg. BW were used to treat this infection for fourteen days orally. The results of the in vivo study indicated that doses of 200 mg/kg BW O.B extract and 6.85 mg/kg. BW of Trimethoprim/Sulfamethoxazole were succeeded in returning the rectal E. coli count of infected rats to normal values before inducing infection in both kinds of therapy at the first week. Food intake, water intake and body weight significantly (P < 0.05) increased in comparison with others doses or groups. From the results obtained, it could be concluded that ethanolic extract of O.B leaves at dose 200 mg/kg. BW was more effective and safe in comparison with antibacterial agent and other O.B dose, this anti-diarrheal activity of O.B ethanolic extract may be due to its constituents of secondary metabolites that are responsible for the antibacterial activity with different mechanisms of action.

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“…These differences in observations could be due to the use of aqueous decoction in the present study compared to the use of organic extracts by other workers. Major bioactive constituents from Z. officinale such as [6]-shogaol, [6]gingerol, [8]-gingerol, and [10]-gingerol, 34 and constituents such as ar-curcumene and α-pinene isolated from volatile oil, 35 have also been reported to have an inhibitory effect on IL-8. However, these belong to the class of constituents that are not soluble in water.…”

Section: Discussionmentioning

confidence: 99%

“…5 There are seven different categories of diarrheagenic E. coli strains based on epidemiology, clinical syndromes, and virulence properties. 6 The enteropathogenic E. coli (EPEC) is an important category that is a leading cause of infantile diarrhea in developing countries. 7 EPEC colonize the intestinal epithelial surface and cause histopathological mucosal changes, the physiological end results of which are disruption of the intestinal epithelial barrier, alterations in intestinal transport, and inflammation.…”

Section: Introductionmentioning

confidence: 99%

Study on effect of anti-diarrheal medicinal plants on enteropathogenic Escherichia coli induced interleukin-8 secretion by intestinal epithelial cells

Brijesh

Tetali²,

Birdi

2011

Altern Med Studies

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Diarrhea is a major health concern in developing countries with enteropathogenic Escherichia coli (EPEC) being a leading cause of infantile diarrhea. Much of the pathology of EPEC infection is due to the inflammatory responses of infected intestinal epithelium through secretion of pro-inflammatory cytokines such as interleukin (IL)-8. With medicinal plants gaining popularity as prospective antidiarrheal agents, we aimed to evaluate the effect of anti-diarrheal medicinal plants on secretion of IL-8 by epithelial cells in response to EPEC infection. The effect of the decoctions of four anti-diarrheal medicinal plants viz. Aegle marmelos, Cyperus rotundus, Psidium guajava and Zingiber officinale was studied on secretion of IL-8 by a human colon adenocarcinoma cell line, HT-29 infected with E. coli E2348/69. Two protocols were used viz. pre-incubation and post-incubation. The data obtained demonstrated that out of the four plants used, only P. guajava decreased secretion of IL-8 in the post-incubation protocol although in the pre-incubation protocol an increase was observed. A similar increase was seen with C. rotundus in the preincubation protocol. No effect on IL-8 secretion was observed with A. marmelos and Z. officinale in both protocols and with C. rotundus in the post-incubation protocol. The post-incubation protocol, in terms of clinical relevance, indicates the effect of the plant decoctions when used as treatment. Hence P. guajava may be effective in controlling the acute inflammatory response of the intestinal epithelial cells in response to EPEC infection.

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R2205 Prevalence of enteropathogenic and shiga toxin-producing Escherichia coli among children with and without diarrhoea in Iran

Cited by 19 publications

References 27 publications

Antibiogram of E. coli serotypes isolated from children aged under five with acute diarrhea in Bahir Dar town

Antibiogram of E. coli serotypes isolated from children aged under five with acute diarrhea in Bahir Dar town

In vitro and in vivo Antibacterial Activity of Ethanolic Extract of Sweet Basil (Ocimum basilicum L.) Leaves against Escherichia coli in Experimentally Infected Rats

Study on effect of anti-diarrheal medicinal plants on enteropathogenic Escherichia coli induced interleukin-8 secretion by intestinal epithelial cells

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