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Przybylska, J.; Zimniak-Przybylska, Z.; Krajewski, Paweł

doi:10.1023/a:1008750423357

Cited by 19 publications

(7 citation statements)

References 12 publications

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“…These results concur with what was observed during the grass pea accessions phenotypic characterization, with lighter and larger accessions looking consistently more resistant to Fop (Sampaio et al, 2021b). Asian and Ethiopian accessions, characterized by darker and smaller seeds (Przybylska et al, 2000), presented in general the lower SNP beneficial alleles frequency. Ethiopian grass pea accessions were also considered the most susceptible in the previous phenotypic characterization to Fop infection, whereas Asian accessions revealed a considerable range of responses, including resistance and susceptibility (Sampaio et al, 2021b).…”

Section: Discussionsupporting

confidence: 91%

“…This habit of eating grass pea in the green state is particularly widespread in Spain, but also in India, Bangladesh, and Pakistan, where the green pods and immature seeds are appreciated for the sweet and tasty flavor and valorized for this feature (Campbell, 1997;Peña-Chocarro & Peña, 1999). Grass pea accessions can be divided into two ecotypes: one with accessions with light and large seeds, typically from Europe and North Africa, and the other with accessions with dark and small seeds, mainly from Asia and Ethiopia (Hanbury et al, 1999;Przybylska et al, 2000). Normally explored for their exceptional resistance to drought, grass pea is also known for their resistance to legume pests and diseases (Campbell, 1997).…”

Section: Introductionmentioning

confidence: 99%

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Grass pea natural variation reveals oligogenic resistance to Fusarium oxysporum f. sp. pisi

Sampaio¹,

Alves²,

Pereira³

et al. 2021

The Plant Genome

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Grass pea (Lathyrus sativus L.) is an annual legume species, phylogenetically close to pea (Pisum sativum L.), that may be infected by Fusarium oxysporum f. sp. pisi (Fop), the causal agent of fusarium wilt in peas with vast worldwide yield losses.A range of responses varying from high resistance to susceptibility to this pathogen has been reported in grass pea germplasm. Nevertheless, the genetic basis of that diversity of responses is still unknown, hampering its breeding exploitation. To identify genomic regions controlling grass pea resistance to fusarium wilt, a genomewide association study approach was applied on a grass pea worldwide collection of accessions inoculated with Fop race 2. Disease responses were scored in this collection that was also subjected to high-throughput based single nucleotide polymorphisms (SNP) screening through genotyping-by-sequencing. A total of 5,651 highquality SNPs were considered for association mapping analysis, performed using mixed linear models accounting for population structure.

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Section: Discussionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Grass pea natural variation reveals oligogenic resistance to Fusarium oxysporum f. sp. pisi

Sampaio¹,

Alves²,

Pereira³

et al. 2021

The Plant Genome

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“…Two main ecotypes can be distinguished. In Europe and North Africa, grass pea seeds are normally light colored and large, while in Asia and Ethiopia seeds are dark colored and relatively small (Hanbury et al 1999;Przybylska et al 2000). Although in Europe grass pea is now mainly used for some traditional gastronomic dishes (Lambein et al 2019), in drought-prone and marginal areas of Africa and South Asia it is a staple food and represents an important source of calories and protein in the diet (Hillocks and Maruthi 2012;Vaz Patto et al 2006a).…”

Section: Introductionmentioning

confidence: 99%

A diversity of resistance sources to Fusarium oxysporum f. sp. pisi found within grass pea germplasm

et al. 2021

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Purpose Grass pea is a legume species with recognized resistance to several diseases and thus important for the improvement of related major legume crops. It is infected by the soil-borne fungus Fusarium oxysporum f. sp. pisi (Fop), known as causal agent of pea fusarium wilt. We aim to identify, among grass pea, new sources of resistance against Fop and characterize the detected resistance mechanisms. Methods A grass pea worldwide collection of accessions was characterized under controlled conditions for response to Fop race 2. Fungal colonization dynamics and potential resistance mechanisms were studied through confocal laser scanning microscopy (CLSM) using Fop race 2 expressing green fluorescent protein (GFP). Results A quantitative nature of resistance to Fop, ranging from highly to partially resistant and susceptible accessions was detected, with resistance being the most frequent phenotype. Diverse colonization patterns were observed, suggesting the existence of different resistance mechanisms. In the highly resistant accessions, absence of fungal colonization in the vascular tissue was detected, while fungal progression was arrested at the level of roots both in highly resistant and partially resistant accessions. Conclusions The resistant accessions identified here can be exploited in grass pea breeding for fusarium wilt resistance, and due to the phylogenetic relatedness to pea, potentially contribute to pea improvement.

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“…This species is seen as a promising source of calories and proteins, and its resilience to adverse abiotic constraints has great potential for expansion in drought-prone and marginal areas ( Vaz Patto and Rubiales, 2014 ; Rubiales et al, 2020 ). Grass pea accessions can be classified into two main ecotypes, mostly considering their seed morphological features: one ecotype with accessions with larger and light-colored seeds usually originated from Mediterranean countries, and the other ecotype with accessions with smaller and dark-colored seeds, mostly from Asian countries ( Przybylska et al, 2000 ). The genetic structure analysis was performed using molecular markers of a worldwide germplasm collection of grass pea accessions ( Sampaio et al, 2021a ).…”

Section: Introductionmentioning

confidence: 99%

Association Mapping of Lathyrus sativus Disease Response to Uromyces pisi Reveals Novel Loci Underlying Partial Resistance

2022

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Uromyces pisi ([Pers.] D.C.) Wint. is an important foliar biotrophic pathogen infecting grass pea (Lathyrus sativus L.), compromising their yield stability. To date, few efforts have been made to assess the natural variation in grass pea resistance and to identify the resistance loci operating against this pathogen, limiting its efficient breeding exploitation. To overcome this knowledge gap, the genetic architecture of grass pea resistance to U. pisi was investigated using a worldwide collection of 182 accessions through a genome-wide association approach. The response of the grass pea collection to rust infection under controlled conditions and at the seedling stage did not reveal any hypersensitive response but a continuous variation for disease severity, with the identification of promising sources of partial resistance. A panel of 5,651 high-quality single-nucleotide polymorphism (SNP) markers previously generated was used to test for SNP-trait associations, based on a mixed linear model accounting for population structure. We detected seven SNP markers significantly associated with U. pisi disease severity, suggesting that partial resistance is oligogenic. Six of the associated SNP markers were located in chromosomes 4 and 6, while the remaining SNP markers had no known chromosomal position. Through comparative mapping with the pea reference genome, a total of 19 candidate genes were proposed, encoding for leucine-rich repeat, NB-ARC domain, and TGA transcription factor family, among others. Results presented in this study provided information on the availability of partial resistance in grass pea germplasm and advanced our understanding of the molecular mechanisms of quantitative resistance to rust in grass pea. Moreover, the detected associated SNP markers constitute promising genomic targets for the development of molecular tools to assist disease resistance precision breeding.

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Cited by 19 publications

References 12 publications

Grass pea natural variation reveals oligogenic resistance to Fusarium oxysporum f. sp. pisi

Grass pea natural variation reveals oligogenic resistance to Fusarium oxysporum f. sp. pisi

A diversity of resistance sources to Fusarium oxysporum f. sp. pisi found within grass pea germplasm

Association Mapping of Lathyrus sativus Disease Response to Uromyces pisi Reveals Novel Loci Underlying Partial Resistance

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