RA promotes proliferation of primordial germ cell‐like cells differentiated from porcine skin‐derived stem cells

Yan, Hong‐Chen; Li, Lin; Liu, Jing‐Cai; Wang, Yufeng; Ge, Wei; Dyce, Paul W.; Li, Lan; Sun, Xiao‐Feng; Shen, Wei; Cheng, Shun‐Feng

doi:10.1002/jcp.28454

Cited by 13 publications

(46 citation statements)

References 46 publications

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“…[ [765][766][767][768][769][770][771][772][773][774] Vitamin B 3 NAD + precursor, it provides sirtuin activity. HDAC III inhibitor, determines heterochromatin remodeling.…”

Section: Vitamins and Their Derivativesmentioning

confidence: 99%

Genome-Protecting Compounds as Potential Geroprotectors

Proshkina

Shaposhnikov

Moskalev

2020

IJMS

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Throughout life, organisms are exposed to various exogenous and endogenous factors that cause DNA damages and somatic mutations provoking genomic instability. At a young age, compensatory mechanisms of genome protection are activated to prevent phenotypic and functional changes. However, the increasing stress and age-related deterioration in the functioning of these mechanisms result in damage accumulation, overcoming the functional threshold. This leads to aging and the development of age-related diseases. There are several ways to counteract these changes: (1) prevention of DNA damage through stimulation of antioxidant and detoxification systems, as well as transition metal chelation; (2) regulation of DNA methylation, chromatin structure, non-coding RNA activity and prevention of nuclear architecture alterations; (3) improving DNA damage response and repair; (4) selective removal of damaged non-functional and senescent cells. In the article, we have reviewed data about the effects of various trace elements, vitamins, polyphenols, terpenes, and other phytochemicals, as well as a number of synthetic pharmacological substances in these ways. Most of the compounds demonstrate the geroprotective potential and increase the lifespan in model organisms. However, their genome-protecting effects are non-selective and often are conditioned by hormesis. Consequently, the development of selective drugs targeting genome protection is an advanced direction.

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“…[ [765][766][767][768][769][770][771][772][773][774] Vitamin B 3 NAD + precursor, it provides sirtuin activity. HDAC III inhibitor, determines heterochromatin remodeling.…”

Section: Vitamins and Their Derivativesmentioning

confidence: 99%

Genome-Protecting Compounds as Potential Geroprotectors

Proshkina

Shaposhnikov

Moskalev

2020

IJMS

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“…Porcine back skin tissue was collected from day 40-45 gestation fetus (E40-45). The isolation, culture and differentiation of pSDSCs were described previously [2,7]. Brie y, the porcine skin tissue was mechanically dissociated using a pipette.…”

Section: Psdscs Isolation Culture and Differentiationmentioning

confidence: 99%

“…Porcine models have close similarities to humans with respect to their genetic relationship and the onset of epigenetic programs, making them ideal animal models for investigating human biology [6]. Porcine skin-derived stem cells (pSDSCs) offer an ideal in vitro model to investigate human ASCs [7]. During last 10 years, germ cells differentiated from pSDSCs have been extensively investigated [2,5,7].…”

Section: Introductionmentioning

confidence: 99%

“…Porcine skin-derived stem cells (pSDSCs) offer an ideal in vitro model to investigate human ASCs [7]. During last 10 years, germ cells differentiated from pSDSCs have been extensively investigated [2,5,7]. But the e ciency of pSDSCs differentiation to porcine primordial germ cell-like cells (pPGCLCs) was very low [7,8].…”

Section: Introductionmentioning

confidence: 99%

“…During last 10 years, germ cells differentiated from pSDSCs have been extensively investigated [2,5,7]. But the e ciency of pSDSCs differentiation to porcine primordial germ cell-like cells (pPGCLCs) was very low [7,8]. This problem can be solved by improving the differentiation e ciency or generating large amounts of pSDSc through enhancing self-renewal.…”

Section: Introductionmentioning

confidence: 99%

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YAP regulates porcine skin-derived stem cells self-renewal partly by repressing Wnt/β-catenin signaling pathway

Yan

Sun

Zhang

et al. 2021

Histochem Cell Biol

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BackgroundSkin-derived stem cells (SDSCs) are a class of adult stem cells (ASCs) that have the ability to self-renew and differentiate. The regulation mechanisms involved in the differentiation of ASCs is a hot topic.Porcine models have close similarities to humans and porcine SDSCs (pSDSCs) offer an ideal in vitro model to investigate human ASCs. To date, studies concerning the role of yes-associated protein (YAP) in ASCs are limited, and the mechanism of its in uence on self-renewal and differentiation of ASCs remain unclear. In this paper, we explore the link between the transcriptional regulator YAP and the fate of pSDSCs. ResultsWe found that YAP promotes the pluripotent state of pSDSCs by maintaining the high expression of the pluripotency genes Sox2, Oct4. The overexpression of YAP prevented the differentiation of pSDSCs and the depletion of YAP by small interfering RNA (siRNAs) suppressed the self-renewal of pSDSCs. In addition, we found that YAP regulates the fate of pSDSCs through a mechanism related to the Wnt/βcatenin signaling pathway. When an activator of the Wnt/β-catenin signaling pathway, CHIR99021, was added to pSDSCs overexpressing YAP the ability of pSDSCs to differentiate was partially restored.Conversely, when XAV939 an inhibitor of Wnt/β-catenin signaling pathway, was added to YAP knockdown pSDSCs a higher self-renewal ability resulted. Conclusionsour results suggested that, YAP and the Wnt/β-catenin signaling pathway interact to regulate the fate of pSDSCs.

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The proliferation role of LH on porcine primordial germ cell‐like cells (pPGCLCs) through ceRNA network construction

Zhang

Tian

Zhang

et al. 2021

Clinical & Translational Med

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Background The transdifferentiation of skin‐derived stem cells (SDSCs) into primordial germ cell‐like cells (PGCLCs) is one of the major breakthroughs in the field of stem cells research in recent years. This technology provides a new theoretical basis for the treatment of human infertility. However, the transdifferentiation efficiency of SDSCs to PGCLCs is very low, and scientists are still exploring ways to improve this efficiency or promote the proliferation of PGCLCs. This study aims to investigate the molecular mechanism of luteinising hormone (LH) to enhance porcine PGCLCs (pPGCLCs) proliferation. Results In this study, we dissected the proliferation regulatory network of pPGCLCs by whole transcriptome sequencing, and the results showed that the pituitary‐secreted reproductive hormone LH significantly promoted the proliferation of pPGCLCs. We combined whole transcriptome sequencing and related validation experiments to explore the mechanism of LH on the proliferation of pPGCLCs, and found that LH could affect the expression of Hippo signalling pathway‐related mRNAs, miRNAs and lncRNAs in pPGCLCs. Conclusions For the first time, we found that LH promotes pPGCLCs proliferation through the competing endogenous RNA (ceRNA) regulatory networks and Hippo signalling pathway. This finding may help to elucidate the molecular mechanism by which LH promotes pPGCLCs proliferation.

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RA promotes proliferation of primordial germ cell‐like cells differentiated from porcine skin‐derived stem cells

Cited by 13 publications

References 46 publications

Genome-Protecting Compounds as Potential Geroprotectors

Genome-Protecting Compounds as Potential Geroprotectors

YAP regulates porcine skin-derived stem cells self-renewal partly by repressing Wnt/β-catenin signaling pathway

The proliferation role of LH on porcine primordial germ cell‐like cells (pPGCLCs) through ceRNA network construction

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