YAP regulates porcine skin-derived stem cells self-renewal partly by repressing Wnt/β-catenin signaling pathway

Yan, Hong‐Chen; Sun, Yu; Zhang, Mingyu; Zhang, Shu-Er; Sun, Jiadong; Dyce, Paul W.; Klinger, Francesca Gioia; Felici, Massimo De; Shen, Wei; Cheng, Shun‐Feng

doi:10.1007/s00418-021-02034-4

Cited by 6 publications

(3 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…According to our KEGG results, the DEGs identified were mainly enriched in the Wnt signaling pathway, which has been shown to play vital roles in epidermal development, stem cell self-renewal, and wound healing [ 55 , 56 , 57 , 58 , 59 , 60 , 61 ]. The horns of ruminants have a common cellular origin from neural crest cells [ 3 ] and the Wnt signaling pathway is essential for regulating the fate, migration, and proliferation of cranial neural crest cells [ 62 , 63 , 64 , 65 ].…”

Section: Discussionmentioning

confidence: 99%

Identification of Critical Genes for Ovine Horn Development Based on Transcriptome during the Embryonic Period

Luan

Wang

et al. 2023

Biology

View full text Add to dashboard Cite

Horns, also known as headgear, are a unique structure of ruminants. As ruminants are globally distributed, the study of horn formation is critical not only for increasing our understanding of natural and sexual selection but also for the breeding of polled sheep breeds to facilitate modern sheep farming. Despite this, a significant number of the underlying genetic pathways in sheep horn remain unclear. In this study, to clarify the gene expression profile of horn buds and investigate the key genes in horn bud formation, RNA-sequencing (RNA-seq) technology was utilized to investigate differential gene expression in the horn buds and adjacent forehead skin of Altay sheep fetuses. There were only 68 differentially expressed genes (DEGs) identified, consisting of 58 up-regulated genes and 10 down-regulated genes. RXFP2 was differentially up-regulated in the horn buds and had the highest significance (p-value = 7.42 × 10−14). In addition, 32 DEGs were horn-related genes identified in previous studies, such as RXFP2, FOXL2, SFRP4, SFRP2, KRT1, KRT10, WNT7B, and WNT3. Further, Gene Ontology (GO) analysis showed that the DEGs were mainly enriched with regard to growth, development, and cell differentiation. Pathway analysis revealed that the Wnt signaling pathway may be responsible for horn development. Further, through combining the protein–protein interaction networks of the DEGs, it was found that the top five hub genes, namely, ACAN, SFRP2, SFRP4, WNT3, and WNT7B, were also associated with horn development. Our results suggest that only a few key genes, including RXFP2, are involved in bud formation. This study not only validates the expression of candidate genes identified at the transcriptome level in previous studies but also provides new possible marker genes for horn development, which may promote our understanding of the genetic mechanisms of horn formation.

show abstract

Section: Discussionmentioning

confidence: 99%

Identification of Critical Genes for Ovine Horn Development Based on Transcriptome during the Embryonic Period

Luan

Wang

et al. 2023

Biology

View full text Add to dashboard Cite

show abstract

“…Transcriptional coactivator with PDZ-binding motif binds SMAD2/3-4 proteins to encourage nuclear agglomeration and coupling to the mediator complex, enhancing their transcriptional activity after TGF stimulation. According to two studies published more recently, YAP is inactivated during (mouse) mESC development, and YAP or TEAD knock-down results in loss of pluripotency (40,41). On the other hand, when it is ectopically overexpressed in induced pluripotent stem cells (iPSCs), activated YAP improves reprogramming effectiveness and prevents differentiation in mESCs (40).…”

Section: Hippo Signaling Pathway In Embryogenesis and Embryonic Stem ...mentioning

confidence: 99%

New Insights into the Roles of the Hippo Signaling Pathway in Stem Cell Biology

Naghsh Nilchi,

Dehghanian,

Khalilian

et al. 2023

Jentashapir J Cell Mol Biol

View full text Add to dashboard Cite

: A highly conserved kinase cascade, including mammalian STE20-like protein kinases (MSTs), large tumor suppressors (LATSs), and downstream transcription coactivators containing YES-associated protein (YAP) and transcriptional coactivator with PDZ-binding motif (TAZ) are involved in the Hippo signaling pathway. As two critical transcriptional coactivators, TAZ and YAP are adversely regulated by the Hippo signaling pathway. They lead to the induction of organ regeneration and the expression of cell growth and division-promoting genes through binding to transcription factors, especially TEA domain transcription factors (TEADs). Previous studies highlight the role of the Hippo pathway in organ size control, homeostasis, cell proliferation, and tumor development. Research has shown this pathway's vital role in cancer stem cell biology, including self-renewal and drug resistance. Improper activation of YAP and TAZ through the Hippo pathway dysregulation or its increased expression can accelerate tumorigenesis. Thus, pharmacological inhibition of YAP and TAZ is suggested as a promising approach in the treatment of tumors with high YAP/TAZ activity.

show abstract

“…Enhanced YAP or TAZ activity is particularly associated with stem and progenitor cell expansion, which is accompanied by the inhibition of differentiation ( Johnson and Halder, 2014 ). These processes have been investigated in mammalian stem and progenitor cells of the intestine, liver, pancreas, heart, and skin ( Camargo et al, 2007 ; Schlegelmilch et al, 2011 ; Heallen et al, 2011 ; von Gise et al, 2012 ; Yan et al, 2022 ). In murine embryonic stem cells, YAP directly binds to the promoters of multiple genes that modulate pluripotency ( Lian et al, 2010 ).…”

Section: Introductionmentioning

confidence: 99%

The Hippo–YAP/TAZ Signaling Pathway in Intestinal Self-Renewal and Regeneration After Injury

Deng

Zou

et al. 2022

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

The Hippo pathway and its downstream effectors, the transcriptional coactivators Yes-associated protein (YAP) and transcriptional coactivator with PDZ-binding motif (TAZ), control stem cell fate and cell proliferation and differentiation and are essential for tissue self-renewal and regeneration. YAP/TAZ are the core components of the Hippo pathway and they coregulate transcription when localized in the nucleus. The intestinal epithelium undergoes well-regulated self-renewal and regeneration programs to maintain the structural and functional integrity of the epithelial barrier. This prevents luminal pathogen attack, and facilitates daily nutrient absorption and immune balance. Inflammatory bowel disease (IBD) is characterized by chronic relapsing inflammation of the entire digestive tract. Impaired mucosal healing is a prominent biological feature of IBD. Intestinal self-renewal is primarily dependent on functional intestinal stem cells (ISCs), especially Lgr5+ crypt base columnar (CBC) cells and transient-amplifying (TA) cells in the crypt base. However, intestinal wound healing is a complicated process that is often associated with epithelial cells, and mesenchymal and immune cells in the mucosal microenvironment. Upon intestinal injury, nonproliferative cells rapidly migrate towards the wound bed to reseal the damaged epithelium, which is followed by cell proliferation and differentiation. YAP is generally localized in the nucleus of Lgr5+ CBC cells, where it transcriptionally regulates the expression of the ISC marker Lgr5 and plays an important role in intestinal self-renewal. YAP/TAZ are the primary mechanical sensors of the cellular microenvironment. Their functions include expanding progenitor and stem cell populations, reprogramming differentiated cells into a primitive state, and mediating the regenerative function of reserve stem cells. Thus, YAP/TAZ play extremely crucial roles in epithelial repair after damage. This review provides an overview of the Hippo–YAP/TAZ signaling pathway and the processes of intestinal self-renewal and regeneration. In particular, we summarize the roles of YAP/TAZ in the phases of intestinal self-renewal and regeneration to suggest a potential strategy for IBD treatment.

show abstract

YAP regulates porcine skin-derived stem cells self-renewal partly by repressing Wnt/β-catenin signaling pathway

Cited by 6 publications

References 37 publications

Identification of Critical Genes for Ovine Horn Development Based on Transcriptome during the Embryonic Period

Identification of Critical Genes for Ovine Horn Development Based on Transcriptome during the Embryonic Period

New Insights into the Roles of the Hippo Signaling Pathway in Stem Cell Biology

The Hippo–YAP/TAZ Signaling Pathway in Intestinal Self-Renewal and Regeneration After Injury

Contact Info

Product

Resources

About