Rab GDP-dissociation inhibitor gdiA is an essential gene required for cell wall chitin deposition in Aspergillus niger

Leeuwe, Tim M. van; Gerritsen, Anne; Arentshorst, Mark; Punt, Peter J.

doi:10.1016/j.fgb.2019.103319

Cited by 15 publications

(12 citation statements)

References 55 publications

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“…Cell wall samples were isolated as previously described ( van Leeuwe et al, 2020 ). In short, dried mycelium was frozen in liquid N 2 and were ground to break open the cells.…”

Section: Methodsmentioning

confidence: 99%

A seven-membered cell wall related transglycosylase gene family in Aspergillus niger is relevant for cell wall integrity in cell wall mutants with reduced α-glucan or galactomannan

et al. 2020

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Highlights Aspergillus niger has seven chitin transglycosylation ( crh ) gene orthologues. All seven crh genes ( crhA-G ) are dispensable for growth and development. Disruption of crhA - G neither weakens the cell wall nor triggers the CWI response. Crh enzymes are relevant for CWI in absence of either α-glucan or galactofuranose. α-Glucan compensates for Crh loss in chitin-to-cell-wall tethering.

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“…Cell wall samples were isolated as previously described ( van Leeuwe et al, 2020 ). In short, dried mycelium was frozen in liquid N 2 and were ground to break open the cells.…”

Section: Methodsmentioning

confidence: 99%

A seven-membered cell wall related transglycosylase gene family in Aspergillus niger is relevant for cell wall integrity in cell wall mutants with reduced α-glucan or galactomannan

et al. 2020

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“…Among these, it is exciting to note that C. rosea sRNA ii_seq_9854567_x248 (up-regulated at contact and after contact in CrFg) was predicted to target F. graminearum transcript XM_011326499.1 coding for a Rab GDP-dissociation inhibitor. The homologue of this gene, whose deletion is lethal, is shown to be involved in chitin deposition in Aspergillus niger (58), and chitin degradation is an important part of C. rosea antagonistic interactions (31, 53), making the sRNA-based cleavage of transcript XM_011326499.1 an interesting hypothesis ( Table 4 ). Similarly, up-regulated milRNA cro-mir-72 was predicted to cleave F. graminearum transcript XM_011323146.1, coding for an elongation factor involved in resistance to oxidative stress in S. cerevisiae (47).…”

Section: Discussionmentioning

confidence: 99%

Comparative small RNA and degradome sequencing provide new insights into antagonistic interactions in the biocontrol fungus Clonostachys rosea

Piombo

Vetukuri

Sundararajan

et al. 2022

Preprint

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Necrotrophic mycoparasitism is an intricate process involving recognition, physical mycelial contact and killing of host fungi (mycohosts). During such interactions, mycoparasites undergo a complex developmental process involving massive regulatory changes of gene expression to produce a range of chemical compounds and proteins that contribute to the parasitism of the mycohosts. Small-RNAs (sRNAs) are vital components of post-transcriptional gene regulation, although their role in gene expression regulation during mycoparasitism remain understudied. Here, we investigated the role of sRNA-mediated gene regulation in mycoparasitism by performing sRNA and degradome tags sequencing of the mycoparasitic fungus Clonostachys rosea interacting with the plant pathogenic mycohosts Botrytis cinerea and Fusarium graminearum at two time points. The majority of differentially expressed sRNAs were down-regulated during the interactions with the mycohosts compared to a C. rosea self-interaction control, thus allowing de-suppression (up-regulation) of mycohost-responsive genes. Degradome analysis showed a positive correlation between high degradome counts and antisense sRNA mapping and led to the identification of 201 sRNA-mediated gene targets for 282 differentially expressed sRNAs. Analysis of sRNA gene targets revealed that the regulation of genes coding for membrane proteins was a common response against both mycohosts. While the regulation of genes involved in oxidative stress tolerance and cellular metabolic and biosynthetic processes was exclusive against F. graminearum highlighting common and mycohosts-specific gene regulation of C. rosea. By combining these results with transcriptome data collected in similar experimental conditions during a previous study, we expand the understanding of the role of sRNA in regulating interspecific fungal interactions and mycoparasitism.

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“…Intriguingly, the Dsk2 mutant exhibits increased tolerance to cell wall perturbing chemicals. The cell wall integrity (CWI) pathway senses hypotonic conditions or multi‐stress including high temperature, oxidative and cell wall stress by controlling cell wall biosynthetic enzymes and expression of cell wall–related genes (Chen et al ., 2014; Jiang et al ., 2020; van Leeuwe et al ., 2020). The increased transcription level of chitin synthesis genes suggested that Dsk2 may be an upstream regulator of the CWI pathway.…”

Section: Discussionmentioning

confidence: 99%

Dsk2 involves in conidiation, multi‐stress tolerance and thermal adaptation in Beauveria bassiana

Wang

Guan

et al. 2021

Environ Microbiol Rep

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Dsk2 is a nuclear-enriched ubiquitin-like polyubiquitinbinding protein that regulates protein degradation in yeast but has not been explored yet in filamentous fungi, such as Beauveria bassiana. Here, we report Beauveria bassiana Dsk2 located both in the nucleus and in cytoplasm of hyphal cells. Deletion of Dsk2 resulted in mild growth defect on scant media with various carbon/nitrogen sources and dramatic attenuation in conidiation capability at optimal condition. Compared to the wild-type, ΔDsk2 strains are much more sensitive to high osmotic and oxidative pressure during vegetative growth. Meanwhile, the mutant strains showed an increased chemical tolerance to Congo red and calcofluor white, two cell wall perturbing agents. The transcriptional changes of genes involved in central development, superoxide dismutase and chitin synthesis pathway indicate that Dsk2 acts as a multifunctional regulator in adapting to environmental changes. Importantly, Dsk2 negatively regulated the ability of thermal resistance in B. bassiana, which makes it a potential target gene for constructing engineering anti-thermal strains in the circumstance of global warming. Altogether, our finding highlights novel roles of Dsk2 involved in the asexual cycle, multi-stress tolerance and pest control potential of B. bassiana.

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Rab GDP-dissociation inhibitor gdiA is an essential gene required for cell wall chitin deposition in Aspergillus niger

Cited by 15 publications

References 55 publications

A seven-membered cell wall related transglycosylase gene family in Aspergillus niger is relevant for cell wall integrity in cell wall mutants with reduced α-glucan or galactomannan

A seven-membered cell wall related transglycosylase gene family in Aspergillus niger is relevant for cell wall integrity in cell wall mutants with reduced α-glucan or galactomannan

Comparative small RNA and degradome sequencing provide new insights into antagonistic interactions in the biocontrol fungus Clonostachys rosea

Dsk2 involves in conidiation, multi‐stress tolerance and thermal adaptation in Beauveria bassiana

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