Rab11-FIP3 binds dynein light intermediate chain 2 and its overexpression fragments the Golgi complex

Rab11-FIP2 influences multiple components of the endosomal system in polarized MDCK cells

“…16 McCaffrey and colleagues have recently noted an association of Rab11-FIP3 with dynein intermediate chain. 18,19 The results here therefore suggest that the Rab11-FIP2 mutants cause retention of dynein on the inhibited recycling system elements perhaps through the retention of Rab11-FIP3.…”

“…17 McCaffrey and colleagues have also recently reported that Rab11-FIP3 interacts with dynein intermediate chains. 18,19 The location of Rab11-FIP2 mutants within the collapsed recycling cisternae near the centrosomes suggested that centripetal dynein motor activity might be concentrating components in a perinuclear membrane nexus. We therefore sought to evaluate whether the Rab11-FIP2 mutants could also alter dynein distribution.…”

The role of motor proteins in endosomal sorting

“…Other work has shown a differential localization of LIC1 and LIC2 as cell progress through mitosis [29]. Rab11 also appears to direct dynein recruitment through its effector FIP3 (Rab11 family-interacting protein 3), but here no LIC specificity is evident [30,31]. These data could be reconciled in many ways.…”

“…The Rab11 effector protein FIP3 binds dynein light chain 2 and form a complex with Rab11 and dynein that mediates transport to the ERC [30,31] a key part of the mechanism of targeting these proteins to cellular membranes. Some SNXs also contain a BAR domain (Bar/amphiphysin/Rvs) that binds to a highly curved membrane, or can impose curvature upon membranes, inducing the generation of tubulovesicular elements.…”

Rhodopsin Trafficking and Mistrafficking

“…66,67 Rab11-FIP3 complex is known to bind the components of cytoplasmic dynein 1. 68,69 Taken together, microtubule-dynein system is a promising mediator of post-Golgi rhodopsin trafficking toward the base of the connecting cilia (Fig. 3).…”