Rab7/Retromer-based endolysosomal trafficking facilitates effector secretion and host invasion in rice blast

Chen, Xin; Selvaraj, Poonguzhali; Lin, Lili; Fang, Wenqin; Wu, Congxian; Yang, Piao; Zhang, Jing; Abubakar, Yakubu Saddeeq; Yang, Fan; Lu, Guang Hong; Liu, Wende; Wang, Zonghua; Naqvi, Naweed I.; Zheng, Weitao

doi:10.1101/2022.06.22.497169

Cited by 5 publications

(18 citation statements)

References 64 publications

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“…Since the start of agriculture, farmers have continuously been improving their practices for combating various plagues suffered by crops [16,173,189,190]. Our growing understanding of the interactions between pathogens and hosts, which began with discovering the causes of plant diseases in the early 19th century, has allowed us to develop many methods for controlling specific plant diseases.…”

Section: Future Directions On Preventive Management Of P Syringaementioning

confidence: 99%

Detection, Diagnosis, and Preventive Management of the Bacterial Plant Pathogen Pseudomonas syringae

Yang

Zhao

Gao

et al. 2023

Plants

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Plant diseases caused by the pathogen Pseudomonas syringae are serious problems for various plant species worldwide. Accurate detection and diagnosis of P. syringae infections are critical for the effective management of these plant diseases. In this review, we summarize the current methods for the detection and diagnosis of P. syringae, including traditional techniques such as culture isolation and microscopy, and relatively newer techniques such as PCR and ELISA. It should be noted that each method has its advantages and disadvantages, and the choice of each method depends on the specific requirements, resources of each laboratory, and field settings. We also discuss the future trends in this field, such as the need for more sensitive and specific methods to detect the pathogens at low concentrations and the methods that can be used to diagnose P. syringae infections that are co-existing with other pathogens. Modern technologies such as genomics and proteomics could lead to the development of new methods of highly accurate detection and diagnosis based on the analysis of genetic and protein markers of the pathogens. Furthermore, using machine learning algorithms to analyze large data sets could yield new insights into the biology of P. syringae and novel diagnostic strategies. This review could enhance our understanding of P. syringae and help foster the development of more effective management techniques of the diseases caused by related pathogens.

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Section: Future Directions On Preventive Management Of P Syringaementioning

confidence: 99%

Detection, Diagnosis, and Preventive Management of the Bacterial Plant Pathogen Pseudomonas syringae

Yang

Zhao

Gao

et al. 2023

Plants

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“…Genome-wide analyses suggest that there are 24 SNARE genes present in the yeast genome (Burri & Lithgow, 2004). However, filamentous fungi typically contain 18-22 SNARE genes (Chen et al, 2023;Gupta & Brent Heath, 2002;Kienle et al, 2009;Yang et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

“…Genome‐wide analyses suggest that there are 24 SNARE genes present in the yeast genome (Burri & Lithgow, 2004). However, filamentous fungi typically contain 18–22 SNARE genes (Chen et al., 2023; Gupta & Brent Heath, 2002; Kienle et al., 2009; Yang et al., 2013). The extended SNARE genes in yeast are a result of whole‐genome duplication within the Saccharomyces lineage (Kienle et al., 2009).…”

Section: Introductionmentioning

confidence: 99%

“…Studies have shown that the exocytic SNAREs in many phytopathogens are important for the fungal development and pathogenicity. In the rice blast fungus Magnaporthe oryzae , Sso1 and Snc1 both play indispensable roles in invasive hyphal development and effector secretion (Chen et al., 2023; Giraldo et al., 2013). Targeted deletions of the SSO1 or SNC1 gene in M. oryzae resulted in a significant reduction in pathogenicity on susceptible rice cultivars (Chen et al., 2023; Giraldo et al., 2013).…”

Section: Introductionmentioning

confidence: 99%

“…In the rice blast fungus Magnaporthe oryzae , Sso1 and Snc1 both play indispensable roles in invasive hyphal development and effector secretion (Chen et al., 2023; Giraldo et al., 2013). Targeted deletions of the SSO1 or SNC1 gene in M. oryzae resulted in a significant reduction in pathogenicity on susceptible rice cultivars (Chen et al., 2023; Giraldo et al., 2013). A recent study found that Sso1–Snc1 interaction is important for the development of the biotrophic interface complex and cytoplasmic effector protein secretion during host infection by M. oryzae (Guo et al., 2023).…”

Section: Introductionmentioning

confidence: 99%

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Two distinct SNARE complexes mediate vesicle fusion with the plasma membrane to ensure effective development and pathogenesis of Fusarium oxysporum f. sp. cubense

Fang,

Zhao,

Yang

et al. 2024

Molecular Plant Pathology

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SNAREs (soluble N‐ethylmaleimide‐sensitive factor attachment protein receptors) facilitate docking and fusion of vesicles with their target membranes, playing a crucial role in vesicle trafficking and exocytosis. However, the spatial assembly and roles of plasma membrane (PM)‐associated SNAREs in phytopathogen development and pathogenicity are not clearly understood. In this study, we analysed the roles and molecular mechanisms of PM‐associated SNARE complexes in the banana Fusarium wilt fungus Fusarium oxysporum f. sp. cubense tropical race 4 (FocTR4). Our findings demonstrate that FocSso1 is important for the fungal growth, conidiation, host penetration and colonization. Mechanistically, FocSso1 regulates protein secretion by mediating vesicle docking and fusion with the PM and hyphal apex. Interestingly, a FocSso1–FocSec9–FocSnc1 complex was observed to assemble not only at the fungal PM but also on the growing hyphal apex, facilitating exocytosis. FocSso2, a paralogue of FocSso1, was also found to form a ternary SNARE complex with FocSec9 and FocSnc1, but it mainly localizes to the PM in old hyphae. The functional analysis of this protein demonstrated that it is dispensable for the fungal growth but necessary for host penetration and colonization. The other subunits, FocSec9 and FocSnc1, are involved in the fungal development and facilitate host penetration. Furthermore, FocSso1 and FocSnc1 are functionally interdependent, as loss of FocSso1 leads to mis‐sorting and degradation of FocSnc1 in the vacuole and vice versa. Overall, this study provides insight into the formation of two spatially and functionally distinct PM SNARE complexes and their involvement in vesicle exocytosis to regulate development and pathogenicity of FocTR4.

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Endocytic dynamin deficiency impairs the host colonization of phytopathogenic fungi

Chen,

Long

et al. 2024

Preprint

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During host-pathogen interactions, pathogens employ endolysosomal trafficking to fine-tune host and environmental cues, facilitating disease progression. Inhibiting the functions of the endolysosomal network could be promising for the control of plant fungal diseases. Herein, we showed that inhibition of the endocytic dynamin FgVps1 by the phenothiazines chlorpromazine (CPZ) and prochlorperazine (PCZ) leads to endolysosomal dysfunction. Further analyses revealed that the phenothiazines directly bind the GTPase domain of FgVps1 to inhibit its activity. Moreover, our results showed that FgVps1 is recruited to the endosome by the Rab GTPases FgRab51 and FgRab7 to facilitate the release of retromer- and sorting nexin-coated vesicles, thereby ensuring the proper recycling of some cargos and promoting fungal development and pathogenicity. Direct treatment of the phytopathogensF. graminearum,Magnaporthe oryzaeandFusarium oxysporum f. sp. cubensewith CPZ and PCZ also impairs their growth, development and virulence. These results unveil the underlying mechanisms of endocytic dynamin actions and provide a potential strategy for broad-spectrum control of phytopathogenic fungi.

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Rab7/Retromer-based endolysosomal trafficking facilitates effector secretion and host invasion in rice blast

Cited by 5 publications

References 64 publications

Detection, Diagnosis, and Preventive Management of the Bacterial Plant Pathogen Pseudomonas syringae

Detection, Diagnosis, and Preventive Management of the Bacterial Plant Pathogen Pseudomonas syringae

Two distinct SNARE complexes mediate vesicle fusion with the plasma membrane to ensure effective development and pathogenesis of Fusarium oxysporum f. sp. cubense

Endocytic dynamin deficiency impairs the host colonization of phytopathogenic fungi

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