Rabbit model for osteoarthrosis of the temporomandibular joint as a basis for assessment of outcomes after intervention

Artuzi, Felipe Ernesto; Langie, Renan Cavalheiro; Abreu, Maíra Cavallet de; Quevedo, Alexandre Silva de; Corsetti, Adriana; Ponzoni, Deise; Puricelli, Edela

doi:10.1016/j.bjoms.2016.01.022

Cited by 12 publications

(13 citation statements)

References 16 publications

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“…An example of this advantage is the possibility to inject substances intra-articularly without the need to access the joint surgically, and this is one of the reasons why rabbit models have emerged as the animal models of choice for the experimental study of diseases and therapies in the human TMJ [42,43]. In addition, we decided to inject MIA directly into the rabbit joint cavity given the results of previous studies confirming the efficacy of OA induction in the TMJ and important late degenerative changes [36,41,44].…”

Section: Discussionmentioning

confidence: 99%

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Reduction of osteoarthritis severity in the temporomandibular joint of rabbits treated with chondroitin sulfate and glucosamine

et al. 2020

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Osteoarthritis is a degenerative disease that causes substantial changes in joint tissues, such as cartilage degeneration and subchondral bone sclerosis. Chondroitin sulfate and glucosamine are commonly used products for the symptomatic treatment of osteoarthritis. The aim of the present study was to investigate the effects of these products when used as structure-modifying drugs on the progression of osteoarthritis in the rabbit temporomandibular joint. Thirty-six New Zealand rabbits were divided into 3 groups (n = 12/group): control (no disease); osteoarthritis (disease induction); and treatment (disease induction and administration of chondroitin sulfate and glucosamine). Osteoarthritis was induced by intra-articular injection of monosodium iodoacetate. Animals were killed at 30 and 90 days after initiation of therapy. The treatment was effective in reducing disease severity, with late effects and changes in the concentration of glycosaminoglycans in the articular disc. The results indicate that chondroitin sulfate and glucosamine may have a structure-modifying effect on the tissues of rabbit temporomandibular joints altered by osteoarthritis. OPEN ACCESSCitation: Artuzi FE, Puricelli E, Baraldi CE, Quevedo AS, Ponzoni D (2020) Reduction of osteoarthritis severity in the temporomandibular joint of rabbits treated with chondroitin sulfate and glucosamine. PLoS ONE 15(4): e0231734. https://doi.org/ 10.

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Section: Discussionmentioning

confidence: 99%

“…CG animals were injected with 50 μL of saline (Fig 1). A 30G needle (8 mm x 0.3 mm) was used to inject the solutions, and the injection site was determined at 5 mm above and distal to the posterior border of the zygomatic process, according to a previously established model [36].…”

Section: Experimental Designmentioning

confidence: 99%

Reduction of osteoarthritis severity in the temporomandibular joint of rabbits treated with chondroitin sulfate and glucosamine

et al. 2020

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“…The transgenic model cannot completely simulate the pathological process of human OA. The KOA model [ 21 , 22 ] can be rapidly created by injecting papain and collagenase into the joint cavity, which causes cartilage damage, and has been commonly used in cartilage pathology and drug treatment research [ 23 ]. However, because of the effects of anatomic site, joint size, and animal species, the drug dose required for modeling was also somewhat different [ 24 , 25 ].…”

Section: Discussionmentioning

confidence: 99%

A Macaca Fascicularis Knee Osteoarthritis Model Developed by Modified Hulth Combined with Joint Scratches

et al. 2018

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BackgroundOsteoarthritis is a common degenerative disease of joints, and animal models have important significance in the investigation of this disease. The aim of this study was to develop a better method for developing osteoarthritis models in primates by comparing the modified Hulth score combined with joint scratches modeling method with others.Material/MethodsWe randomly divided 15 young male Macaca fascicularis and 3 old male Macaca fascicularis into 6 groups (n=3). Knee osteoarthritis (KOA) models were developed with different methods: modified Hulth combined with joint scratches (Group A), modified Hulth (Group B), Hulth (Group C), spontaneous models (Group D); sham-operated (Group E), and blank control (Group F). Morphology and pathology of knee joints were observed at the 8th week after surgery. The levels of WBC, IL-1β, and TGF-β1 in synovial fluid were detected by ELISA. The levels of COL-II, ACAN, and MMP-13 in articular cartilage were examined by RT-qPCR and Western blot.ResultsIn Brittberg and modified Mankin score, Group A was higher than B (P<0.05) and lower than C (P<0.05), and there was no statistically significant difference between Group A and D (P>0.05). Except for Group E and F, the differences were statistically significant among others in WBC, IL-1β, and TGF-β1 (P<0.05). COL-II and ACAN decreased and MMP-13 increased, and there was no significant difference between Groups A and D (P>0.05) or between Groups E and F (P>0.05). There were statistically significant differences among other groups (P<0.05).ConclusionsThe models developed by modified Hulth combined with joint scratches were the closet to spontaneous models at the 8th week after surgery.

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“…MIA (Cledes et al;Güler et al;Artuzi et al, 2016) and papain (Yang & Shi) have been used successfully to induce OA in rabbit TMJs in previous studies. The severity of the OA is directly related with the exposure time (Cledes et al) and the concentration of the drug applied (Guingamp et al, 1997;Güler et al).…”

Section: Discussionmentioning

confidence: 99%

CBCT Analysis of Rabbit Temporomandibular Joints With Osteoarthrosis Induced by Monoiodoacetate and Papain

et al. 2018

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ALVES, N.; GARAY, I.; DEANA, N. & MOLINETT, M. CBCT analysis of rabbit temporomandibular joints with osteoarthrosis induced by monoiodoacetate and papain. Int. J. Morphol., 36(2):513-518, 2018. SUMMARY: Osteoarthrosis (OA) is a degenerative disease characterized by loss of joint cartilage, remodelling of the subchondral bone, narrowing of joint spaces and the formation of osteophytes. Animal models are used to study the mechanisms of OA, as well as to test the effects of anti-osteoarthrosis drugs. The objective of the present study was to determine the changes identifiable by imaging techniques occurring in rabbit temporomandibular joints (TMJ) at 15, 25 and 45 days after OA inducement by monoiodoacetate (MIA) and papain. The imaging technology used was cone-beam computerised tomography (CBCT). The model animals were 22 young adult male New Zealand rabbits, divided randomly into three study groups: Four rabbits in the control group, nine in the papain experimental group and nine in the monoiodoacetate (MIA) experimental group. OA was induced by arthrocentesis in the lower compartment of both TMJs. The rabbits were examined by CBCT at 15, 25 and 45 days after the injection of MIA and papain. The mandibular condyles presented loss of their rounded shape, deformation of the condyle or mandibular fossa, cortical irregularity, cortical wear and changes in the dimensions of the condyle. OA induction by MIA and papain generates changes observable by CBCT in the dimensions of the mandibular condyle in rabbits. Both inducers promote signs compatible with OA on the joint surfaces of the TMJ; MIA promotes more expressive changes. ALVES, N.; GARAY, I.; DEANA, N. & MOLINETT, M. CBCT analysis of rabbit temporomandibular joints with osteoarthrosis induced by monoiodoacetate and papain.Int. J. Morphol., 36(2):513-518, 2018.

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Rabbit model for osteoarthrosis of the temporomandibular joint as a basis for assessment of outcomes after intervention

Cited by 12 publications

References 16 publications

Reduction of osteoarthritis severity in the temporomandibular joint of rabbits treated with chondroitin sulfate and glucosamine

Reduction of osteoarthritis severity in the temporomandibular joint of rabbits treated with chondroitin sulfate and glucosamine

A Macaca Fascicularis Knee Osteoarthritis Model Developed by Modified Hulth Combined with Joint Scratches

CBCT Analysis of Rabbit Temporomandibular Joints With Osteoarthrosis Induced by Monoiodoacetate and Papain

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