Rabbit model of Lyme borreliosis: erythema migrans, infection-derived immunity, and identification of Borrelia burgdorferi proteins associated with virulence and protective immunity.

Foley, Denise; Gayek, Richard J.; Skare, Jon T.; Wagar, Elizabeth A.; Champion, Cheryl I.; Blanco, David R.; Lovett, Michael; Miller, James N.

doi:10.1172/jci118144

Cited by 42 publications

(86 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Histopathologically, EM is marked by perivascular infiltration of lymphocytes and plasma cells with few macrophages and scattered mast cells (8). Similar skin lesions were reported in both monkey and rabbit models of Lyme borreliosis (9,10). In the monkey, the histopathology of EM consists of macrophage and lymphocyte infiltration, accompanied by a prominent eosinophil and an occasional basophil response (9).…”

Section: Increasing the Recruitment Of Neutrophils To The Site Of Infmentioning

confidence: 54%

“…In the monkey, the histopathology of EM consists of macrophage and lymphocyte infiltration, accompanied by a prominent eosinophil and an occasional basophil response (9). In the rabbit EM lesion, the infiltration is a mixed lymphocyte population with occasional macrophages and plasma cells (10). Apparently, EM results from the inflammatory response triggered by spirochete replication and develops to contain infection.…”

Section: Increasing the Recruitment Of Neutrophils To The Site Of Infmentioning

confidence: 99%

See 1 more Smart Citation

Increasing the Recruitment of Neutrophils to the Site of Infection Dramatically Attenuates Borrelia burgdorferi Infectivity

Seemanapalli

Reif

et al. 2007

The Journal of Immunology

View full text Add to dashboard Cite

Borrelia burgdorferi infection causes an initial skin lesion called erythema migrans (EM) in human Lyme disease and in models of monkey and rabbit borreliosis. EM results from the inflammatory response triggered by spirochete replication and likely develops to contain the initial infection but allows bacterial dissemination to occur. The essential lack of neutrophil involvement in EM histopathology prompted us to examine the consequence of increasing their recruitment in the inflammatory response to the Lyme disease agent. B. burgdorferi was modified genetically to constitutively express and secrete the chemokine KC, a neutrophil chemoattractant. After inoculation into the dermis of the murine host, control spirochetes induced an infiltration of macrophages, neutrophils, and basophils within 6 h; however, the recruited neutrophils and basophils were quickly substituted by eosinophils, and the inflammatory response became macrophage dominant by 16 h. Such a response failed to contain the initial infection and allowed the spirochetes to disseminate. In contrast, B. burgdorferi with KC secretion induced an intensive neutrophil infiltration at the inoculation site, and as a result, the host’s ability to control the initial infection was greatly enhanced. Taken together, this study suggests that the failure of sufficient neutrophil recruitment and activation during the initial inflammatory response may allow B. burgdorferi to effectively colonize the mammalian host.

show abstract

Section: Increasing the Recruitment Of Neutrophils To The Site Of Infmentioning

confidence: 54%

Section: Increasing the Recruitment Of Neutrophils To The Site Of Infmentioning

confidence: 99%

Increasing the Recruitment of Neutrophils to the Site of Infection Dramatically Attenuates Borrelia burgdorferi Infectivity

Seemanapalli

Reif

et al. 2007

The Journal of Immunology

View full text Add to dashboard Cite

show abstract

“…B. burgdorferi sensu stricto strain B31 was used in the experiments presented in this report and will be referred to as B. burgdorferi strain B31 throughout this manuscript. Virulent B. burgdorferi was originally isolated from infected mouse or rabbit tissue as previously described (26). The term "passage" and the corresponding number refers to the number of times a B31 B. burgdorferi log phase culture was transferred to fresh BSK II media.…”

Section: Methodsmentioning

confidence: 99%

“…The term "passage" and the corresponding number refers to the number of times a B31 B. burgdorferi log phase culture was transferred to fresh BSK II media. Previous studies indicated that B. burgdorferi strain B31 maintained infectivity in rabbits and mice to at least passage 47 and 85, respectively (26). The avirulent B. burgdorferi strain B31 (American Type Culture Collection [ATCC] 35210, Rockville, MD) has been passaged several hundred times in vitro in our laboratory and is noninfectious for both mice and rabbits (13,26).…”

Section: Methodsmentioning

confidence: 99%

Virulent strain associated outer membrane proteins of Borrelia burgdorferi.

Skare¹,

Shang²,

Foley³

et al. 1995

J. Clin. Invest.

Self Cite

159

View full text Add to dashboard Cite

We have isolated and purified outer membrane vesicles (OMV) from Borrelia burgdorferi strain B31 based on methods developed for isolation of Treponema pallidum OMV. Purified OMV exhibited distinct porin activities with conductances of 0.6 and 12.6 nano-Siemen and had no detectable /8-NADH oxidase activity indicating their outer membrane origin and their lack of inner membrane contamination, respectively. Hydrophobic proteins were identified by phase partitioning with Triton X-114. Most of these hydrophobic membrane proteins were not acylated, suggesting that they are outer membrane-spanning proteins. Identification of palmitate-labeled lipoproteins revealed that several were enriched in the OMV, several were enriched in the protoplasmic cylinder inner membrane fraction, and others were found exclusively associated with the inner membrane. The protein composition of OMV changed significantly with successive in vitro cultivation of strain B31. Using antiserum with specificity for virulent strain B31, we identified OMV antigens on the surface of the spirochete and identified proteins whose presence in OMV could be correlated with virulence and protective immunity in the rabbit Lyme disease model. These virulent strain associated outer membrane-spanning proteins may provide new insight into the pathogenesis of Lyme disease. (J. Clin. Invest.

show abstract

“…Chez l'animal, seuls le lapin et le singe Rhésus sont décrits comme dévelop-pant un érythème migrant suite à l'inoculation de Borrelia (Philipp & Johnson, 1994 ;Foley et al 1995).…”

Section: Manifestations Cliniquesunclassified

Borréliose de Lyme : impact de l’homme sur la persistance et la circulation de Borrelia Burgdorferi sensu lato dans l’environnement

Boulanger¹,

Jaulhac²

2015

bavf

View full text Add to dashboard Cite

La borréliose de Lyme est une infection bactérienne transmise par les tiques dures du genre Ixodes. C'est avant tout une zoonose qui circule dans un large panel d'hôtes vertébrés sauvages (lézards, oiseaux, rongeurs, cervidés…) et domestiques ; l'homme est un hôte accidentel. Ces dernières années, la notion d'émergence a été évoquée pour cette pathologie, notamment à cause des modifications climatiques. Les raisons sont plus complexes. La pathologie est mieux connue sur un plan médical grâce aux progrès diagnostiques, cliniques et épidémiologiques. De plus, l'analyse des différents facteurs biotiques et abiotiques laissent penser que l'anthropisation des environnements où évoluent les tiques, est responsable de l'émergence des maladies transmises par les tiques dont la borréliose de Lyme. En effet, l'homme modifie pour des raisons socio-économiques surtout, les écosystèmes forestiers et la faune sauvage qui favorisent le développement des populations de tique et donc des maladies qu'elles transmettent. Mots

show abstract

Rabbit model of Lyme borreliosis: erythema migrans, infection-derived immunity, and identification of Borrelia burgdorferi proteins associated with virulence and protective immunity.

Cited by 42 publications

References 28 publications

Increasing the Recruitment of Neutrophils to the Site of Infection Dramatically Attenuates Borrelia burgdorferi Infectivity

Increasing the Recruitment of Neutrophils to the Site of Infection Dramatically Attenuates Borrelia burgdorferi Infectivity

Virulent strain associated outer membrane proteins of Borrelia burgdorferi.

Borréliose de Lyme : impact de l’homme sur la persistance et la circulation de Borrelia Burgdorferi sensu lato dans l’environnement

Contact Info

Product

Resources

About