Rabbit red blood cell stroma bind immunoglobulin M antibodies regardless of blood group specificity

Storry, Jill R.; Olsson, Martin L.; Moulds, John J.

doi:10.1111/j.1537-2995.2006.00879.x

Cited by 5 publications

(7 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Notably, in a report by Mechanic and coworkers 11 all eight stored sera with anti‐Vel tested showed some reduction in reactivity after adsorption. Because anti‐Vel antibodies are somewhat unusual in that they are generally a mixture of warm‐reactive IgM and IgG, the adsorption of anti‐Vel in these cases was felt by Storry and colleagues to be due to the fact that rabbit RBC stroma adsorbs IgM antibodies nonspecifically, regardless of RBC antigen specificity 13 . In their report, they sought to confirm this hypothesis by showing that the diminished anti‐Vel reactivity was seen mainly in the saline agglutination phase of testing and that two examples of monoclonal IgM anti‐D and anti‐K were adsorbed by RESt with significant reduction in titers, but not the two polyclonal IgG anti‐D and anti‐K, which had no change in titer or only a twofold reduction.…”

Section: Discussionmentioning

confidence: 99%

“…The results of the Storry group and their conclusion that IgM antibodies were removed merit attention, because it was the first time to our knowledge that monoclonal IgM antibodies were used to assess the effect of RESt on IgM antibodies specifically, free of “interferences” from IgG antibodies. As a result of the study by Storry and coworkers it was stated in the most recent edition of Judd's Methods in Immunohematology that adsorbing cold autoantibodies with rabbit RBCs may “adsorb alloantibodies of potential clinical significance if they are IgM.” 19 However, only two examples of monoclonal IgM antibodies were examined 13 . We included 12 monoclonal IgM antibodies of various antigen specificities in this study and showed that most, but not all IgM alloantibodies were adsorbed by RESt with significant reductions in antibody titers.…”

Section: Discussionmentioning

confidence: 99%

“…More recently, in a study by Storry and coworkers, 13 it was proposed based on limited data that RESt adsorbs IgM antibodies, regardless of specificity. This interesting hypothesis, if confirmed, would provide not only an explanation of how IgM cold autoantibodies are removed, but also an explanation for the occasional removal of clinically significant alloantibodies.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Immunoglobulin M red blood cell alloantibodies are frequently adsorbed by rabbit erythrocyte stroma

et al. 2010

View full text Add to dashboard Cite

BACKGROUND: Rabbit erythrocyte stroma (RESt, Immucor) adsorption is often used to remove cold autoantibodies from patient samples to facilitate detection of underlying alloantibodies. However, reports in the literature show that adsorption of clinically significant alloantibodies can occur. A 2006 study by Storry and colleagues suggested that immunoglobulin (Ig)M antibodies are adsorbed by RESt regardless of antigen specificity. In our study, we further investigated the adsorption of IgM red blood cell alloantibodies by RESt. STUDY DESIGN AND METHODS: A total of 12 sera containing monoclonal IgM antibodies of various specificities (anti-D-Jk b , and -S) and titers, which were all shown to exhibit only IgM reactivity after dithiothreitol treatment, and two sera with polyclonal IgG (anti-Fy a and -K) were all adsorbed by RESt. Titers of unadsorbed, once-adsorbed, and twiceadsorbed IgM and IgG antibodies were determined in parallel. RESULTS: Ten of the 12 monoclonal IgM samples showed significant (more than fourfold) reduction in titer after RESt adsorptions. Both of the polyclonal IgG samples tested showed insignificant (fourfold or less) reduction in titer. CONCLUSIONS: RESt is known to effectively remove IgM cold autoantibodies. Our results show that monoclonal IgM alloantibodies are also frequently adsorbed by RESt with significant reduction in titer. Adsorption is variable and some IgM alloantibodies are not adsorbed. Further studies may elucidate the effect of RESt adsorption on IgG alloantibodies. Caution is needed when RESt is employed to remove interferences by cold autoantibodies in pretransfusion testing, and the risk of missed IgM alloantibodies must be considered.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Immunoglobulin M red blood cell alloantibodies are frequently adsorbed by rabbit erythrocyte stroma

et al. 2010

View full text Add to dashboard Cite

show abstract

“…Many Vel antibodies are readily adsorbed by rabbit RBCs or red cell stroma, although this is apparently not a specific adsorption but more that these reagents preferentially adsorb IgM antibodies. 26,27 The ability to hemolyze Vel+ RBCs, at least in the days when serum was used for testing, has been a hallmark of anti-Vel and, in his review, Sussman reported that anti-Vel from 12 of 19 patients hemolyzed RBCs in vitro. 2 The serological reactivity of anti-Vel with RBCs treated with papain, ficin, trypsin, and α-chymotrypsin is greatly enhanced, and serum containing anti-Vel will often readily hemolyze papain-treated RBCs, for example.…”

Section: Antibodies In the Systemmentioning

confidence: 99%

The Vel blood group system: a review

Storry

Peyrard

2017

Immunohematology

View full text Add to dashboard Cite

The blood group antigen Vel has been one of immunohematology's greatest enigmas: the variation in antigen strength from one individual to another, the property of anti-Vel to readily hemolyze Vel+ red blood cells (RBCs), and the difficulty to screen for sufficient numbers of Vel-blood donors had made Vel a tough nut to crack. In 2013, a small, previously unknown protein called small integral membrane protein 1 (SMIM1) was identified on the RBC by three independent research groups using different approaches, and all three groups demonstrated that Vel-RBCs lacked SMIM1. This discovery correlated with homozygosity for deletion c.64_60del in SMIM1 and meant that for the first time there was a universal method to screen for Vel-blood donors. This finding was not the whole answer, however, and an explanation behind the variability in antigen strength was later shown to be due to polymorphism in SMIM1 intron 2, a region that is responsible for gene transcription. Clinically, anti-Vel is important and has caused severe transfusion reactions, although hemolytic disease of the fetus and newborn caused by anti-Vel is uncommon. However, while screening for Vel-blood donors has become easier, the function of SMIM1 is still unknown, and despite its well-conserved sequence across the animal kingdom, the enigma continues.

show abstract

“…According to the types and amount of antigen and antibody, blood is classified into more than 30 different types. 4,5 In general, blood types are categorized by the ABO (A, B, O, and AB) and Rh (Rh+ and Rh−) blood types. 6 The ABO blood type is determined according to the antigen on the red blood cells (RBCs) and the antibody in the plasma, whereas the Rh blood type is determined whether the RBCs have the D antigen (Rh+) or not (Rh−).…”

Section: Introductionmentioning

confidence: 99%

Fully-automatic blood-typing chip exploiting bubbles for quick dilution and detection

2020

View full text Add to dashboard Cite

A compact, fully-automatic blood-typing test device is developed. The device conducts sequential processes of whole-blood dilution, homogenization, and reaction with reagents. The lab-on-a-chip device can detect the weakest reaction between red blood cells (RBCs) and reagents even without using optics such as a camera and detector. This high sensitivity is achieved by implementing 50- μ m-thick reaction chambers in which a clear contrast between the RBC agglutinations and non-reacted RBCs can be obtained. The dilution and the homogenization are enhanced by injecting bubbles into the microchannel so that the test result can be obtained 5 min after the test start. With an assumption that the device will be used by medical staffs, the device is designed to require minimum operation for the users, namely, loading whole blood, starting pumps, and looking inside the reaction chambers by their eyes to observe the test result. As the device is applicable to the cross-matching test by mixing RBCs with serum instead of the reagents, it is expected that the device provides not only the quick blood-typing but also a safer and quicker blood transfusion in emergency rooms.

show abstract

Rabbit red blood cell stroma bind immunoglobulin M antibodies regardless of blood group specificity

Cited by 5 publications

References 4 publications

Immunoglobulin M red blood cell alloantibodies are frequently adsorbed by rabbit erythrocyte stroma

Immunoglobulin M red blood cell alloantibodies are frequently adsorbed by rabbit erythrocyte stroma

The Vel blood group system: a review

Fully-automatic blood-typing chip exploiting bubbles for quick dilution and detection

Contact Info

Product

Resources

About