Rabbit Urethra Replacement with a Defined Biomatrix or Small Intestinal Submucosa

Nuininga, Jody; Moerkerk, Hilco van; Hanssen, Alex; Hulsbergen, C.A.; Oosterwijk-Wakka, J.C.; Oosterwijk, Egbert; Gier, R.P.E. de; Schalken, Jack A.; Kuppevelt, Toin van; Feitz, W.F.J.

doi:10.1016/s0302-2838(03)00249-5

Cited by 47 publications

(35 citation statements)

References 27 publications

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“…The resemblance in histological fi ndings between both biomatrices is in accordance with a previous study [19]. Both biomatrices were incorporated well into the surrounding tissues and ingrowth of fi broblasts was demonstrated.…”

Section: Discussionsupporting

confidence: 91%

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Histological Evaluation of Acute Covering of an Experimental Neural Tube Defect with Biomatrices in Fetal Sheep

Eggink

Roelofs²,

Lammens³

et al. 2006

Fetal Diagn Ther

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Objective: The aim of the study was to determine the histological effect on the neural tissue of in utero covering of an experimental neural tube defect in fetal lambs, with the use of two different biomatrices. Materials and Methods: In 23 fetal sheep, surgery was performed at 79 days’ gestation. In 19 of these, a neural tube defect was created, while 4 fetuses served as sham-operated controls. In 7 of the 19 operated fetuses the defect was left uncovered. In the remaining 12 animals the defect was covered either with a collagen biomatrix (4 animals), skin (3 animals), or small intestinal submucosa biomatrix (5 animals). The lambs were sacrificed at 1 week of age and histological examination was performed. Results: All lambs with an uncovered neural tube defect showed histological damage of the spinal cord. In lambs in which the neural tube defect was covered, one half showed a normal architecture of the spinal cord while minor histological damage was present in the other half. Between the three groups in which the defect was covered, the histological outcome was comparable. Conclusions: Acute covering of an experimental neural tube defect in fetal lambs prevents severe histological damage to the spinal cord independent of the two biomatrices used in this study.

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Section: Discussionsupporting

confidence: 91%

“…We used a novel, molecularly defi ned, collagen-based, biocompatible and biodegradable biomatrix and compared it, because of earlier experience in an animal model, with an SIS biomatrix [19] . The UMC biomatrix is made from insoluble type I collagen and after preparation the isolated collagen is free of other proteins.…”

Section: Discussionmentioning

confidence: 99%

Histological Evaluation of Acute Covering of an Experimental Neural Tube Defect with Biomatrices in Fetal Sheep

Eggink

Roelofs²,

Lammens³

et al. 2006

Fetal Diagn Ther

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“…In this model we evaluated a novel molecularly defi ned UMC biomatrix and compared this with an SIS biomatrix, a xenogenic membrane that is composed mainly of the submucosal layer of the intestinal wall harvested from porcine small intestine. These biomatrices have been used earlier, especially for urogenital reconstructions in animals [14,15] . In this study we used the simplest defi ned biomatrix and the results of the UMC biomatrix were comparable to the SIS.…”

Section: Discussionmentioning

confidence: 99%

In utero Repair of an Experimental Neural Tube Defect in a Chronic Sheep Model Using Biomatrices

Eggink

Roelofs²,

Feitz³

et al. 2005

Fetal Diagn Ther

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Objective: Persistent exposure of the unprotected spinal cord to amniotic fluid and the uterine wall can lead to progressive damage of neural tissue in case of a myelomeningocele (two-hit hypothesis). The aim of this study was to evaluate whether in utero repair of an experimental neural tube defect in a fetal lamb could protect neural tissue from secondary injury and save neurologic functions after birth. Methods:In 19 fetal lambs, a neural tube defect was created at 79 days’ gestation. In 12 lambs the defect was covered either with a novel, molecular defined collagen-based biocompatible and biodegradable matrix (UMC) or with a small intestinal submucosa (SIS) biomatrix (Cook^®) or by closing the skin over the defect. Results: All lambs with the defect covered showed no or minor neurologic morbidity in contrast to the lambs with the defect uncovered in which major neurologic morbidity was seen. Conclusions:These results demonstrate that long-term exposure of the open spinal cord to the intrauterine environment can lead to damage of neural tissue and, consequently loss of neurologic functions and that coverage of the defect can lead to a better neurologic outcome. Furthermore, we could show that a UMC biomatrix and an SIS biomatrix are useful for in utero coverage of a surgically created neural tube defect in our model.

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“…103 However, whereas the gal epitope has been shown to be present in the acellular matrix scaffold material, 104 most preclinical studies using acellular matrix scaffolds have failed to show convincing evidence of an adverse immunologic response and tissue remodeling. [105][106][107] Additionally, pigs have now been created with genetic modifications to reduce xenogenic rejection in humans. [108][109][110] For example, the alpha1,3-galactosyltransferase gene-knockout pig has been engineered to ensure more complete immunogenicity-deleted lungs.…”

Section: Candidate Species For Decellularized Scaffolds and Transplanmentioning

confidence: 99%

Future prospects for tissue engineered lung transplantation

et al. 2014

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Rabbit Urethra Replacement with a Defined Biomatrix or Small Intestinal Submucosa

Cited by 47 publications

References 27 publications

Histological Evaluation of Acute Covering of an Experimental Neural Tube Defect with Biomatrices in Fetal Sheep

Histological Evaluation of Acute Covering of an Experimental Neural Tube Defect with Biomatrices in Fetal Sheep

In utero Repair of an Experimental Neural Tube Defect in a Chronic Sheep Model Using Biomatrices

Future prospects for tissue engineered lung transplantation

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