2016
DOI: 10.1021/acsami.6b06415
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“Race for the Surface”: Eukaryotic Cells Can Win

Abstract: With an aging population and the consequent increasing use of medical implants, managing the possible infections arising from implant surgery remains a global challenge. Here, we demonstrate for the first time that a precise nanotopology provides an effective intervention in bacterial cocolonization enabling the proliferation of eukaryotic cells on a substratum surface, preinfected by both live Gram-negative, Pseudomonas aeruginosa, and Gram-positive, Staphylococcus aureus, pathogenic bacteria. The topology of… Show more

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Cited by 102 publications
(92 citation statements)
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References 38 publications
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“…Fraioli et al used nanostructured titanium surfaces to encourage osteogenesis in human mesenchymal stem cells, while simultaneously acting as an antibacterial surface to a multidrug‐resistant bacteria ( Pseudomonas aeruginosa ) . Similarly, black silicon has been shown to inhibit the growth of pathogenic bacteria (a live coculture of Pseudomonas aeruginosa and Staphylococcus aureus ), while simultaneously supporting the proliferation of fibroblast cells (COS‐7) …”
Section: Prokaryotic Cell Interfacingmentioning
confidence: 99%
“…Fraioli et al used nanostructured titanium surfaces to encourage osteogenesis in human mesenchymal stem cells, while simultaneously acting as an antibacterial surface to a multidrug‐resistant bacteria ( Pseudomonas aeruginosa ) . Similarly, black silicon has been shown to inhibit the growth of pathogenic bacteria (a live coculture of Pseudomonas aeruginosa and Staphylococcus aureus ), while simultaneously supporting the proliferation of fibroblast cells (COS‐7) …”
Section: Prokaryotic Cell Interfacingmentioning
confidence: 99%
“…Importantly, for implantbased applications, nanostructured antibacterial technologies need only to aid osteointegration by reducing the probability of infection. 50,51 Here, the so-called "race to the surface" becomes important, 52 where host cells and pathogenic species compete for surface colonisation at the implant-tissue interface. Competitive proliferation studies 50,51 have highlighted the viability of similar surface architectures, demonstrating that mammalian cells were capable of growing over, and successfully colonizing, pre-infected substrata possessing antibacterial surface nanostructures.…”
Section: Concentrationsmentioning
confidence: 99%
“…Moreover, we wanted to evaluate bacteria and MC3T3-E1 cell adhesion to the biomaterial when reaching the surface simultaneously. This was unlike other previously reported studies, in which the material was infected prior to the adhesion of eukaryotic cells 12,13,26-29 (so that bacteria had an advantage to win the race from the beginning), or after the integration of the tissue on the implant (so that cells had such an advantage). 30 …”
Section: Discussionmentioning
confidence: 56%
“…12,13,25-28,12,13,26-29 In this study, we chose five concentrations for collection strains (10 4 , 10 5 , 10 6 , 10 7 , 10 8 CFU/mL) but six (10 3 , 10 4 , 10 5 , 10 6 , 10 7 , 10 8 CFU/mL) for clinical strains as they show high adherence to the surface, in the presence of a constant concentration of osteoblasts (10 5 cells/mL). Although most of the bacteria strains tested had maximal adhesion to CP disks at 10 8 CFU/mL, for P1 and P18 this corresponding value was attained at 10 6 and 10 7 CFU/mL, respectively.…”
Section: Discussionmentioning
confidence: 99%