RAD10, an excision repair gene of Saccharomyces cerevisiae, is involved in the RAD1 pathway of mitotic recombination.

Abstract

The RADIO gene of Saccharomyces cerevisiae is required for the incision step of excision repair of UV-damaged DNA. We show that the RADIO gene is also required for mitotic recombination. The radlOA mutation lowered the rate of intrachromosomal recombination of a his3 duplication in which one his3 allele has a deletion at the 3' end and the other his3 allele has a deletion at the 5' end (his3A3' his3A5'). The rate of formation of HIS3+ recombinants in the radlOA mutant was not affected by the radlA mutation but decreased synergistically in the presence of the radlOA mutation in combination with the rad52A mutation. These observations indicate that the RADI and RADIO genes function together in a mitotic recombination pathway that is distinct from the RADS2 recombination pathway. The radiOA mutation also lowered the efficiency of integration of linear DNA molecules and circular plasmids into homologous genomic sequences. We suggest that the RADI and RADIO gene products act in recombination after the formation of the recombinogenic substrate. The radiA and radlOA mutations did not affect meiotic intrachromosomal recombination of the his3A3' his3A5' duplication or mitotic and meiotic recombination of ade2 heteroalleles located on homologous chromosomes.In the yeast Saccharomyces cerevisiae, excision repair of DNA damaged by UV light and bulky adducts that distort the DNA helix is controlled by at least 10 different genes. Mutations in the RADI, RAD2, RAD3, RAD4, and RADIO genes abolish the incision of damaged DNA (17,29,43), whereas mutations in the RAD7, RAD14, RAD16, RAD23, and MMSJ9 genes affect the proficiency of excision repair (17,18,43). With the goal of purifying and characterizing the protein components required for excision repair, we have cloned many of these genes and have purified and characterized the RAD3-encoded protein (40-42). The cloned genes have also been used to construct genomic deletion mutations. Studies with these mutations have revealed that RAD3 is essential for cell viability (8,20) and that RADI functions in mitotic recombination (12,34). Thus, in contrast to Escherichia coli, in which the uvrA, uvrB, and uvrC genes are required only for the incision of UV-damaged DNA, some of the yeast excision repair genes affect other cellular processes.Previously, we showed that the radlA mutation lowers the rate of mitotic intrachromosomal recombination of a his3 duplication in which one of the his3 alleles has a deletion at the 3' end and the other has a deletion at the 5' end (34). The RAD52 gene, required for double-strand break repair and recombination (23), is also involved in this recombination. However, the RADI and RADS2 genes affect mitotic recombination via alternate pathways, since the formation of HIS3+ recombinants is decreased synergistically in the radlA rad52A double mutant (34). Based on the observation that the radiA mutation reduces the efficiency of integration of linear plasmids and DNA fragments into homologous genomic sequences, we have suggested that the action of RADI in rec...

Correction of xeroderma pigmentosum repair defect by basal transcription factor BTF2 (TFIIH).

“…Thus these results rule out the theoretical possibility raised by these and other remarkable observations with respect to the ERCC3 gene in the yeast and Drosophila systems, namely that the ERCC3 -NER connection could be indirect, i.e. ERCC3 might control the expression of one or more (real) NER genes without being involved itself in this process (Gulyas and Donahue, 1992;Mounkes et al, 1992;Schaeffer et al, 1993 (Schiestl and Prakash, 1990;Bailly et al, 1992;Bardwell et al, 1992;Biggerstaff et al, 1993;van Vuuren et al, 1993). Using the in vitro NER assay Wood and coworkers have shown that the replication factors PCNA and HSSB (RPA) also participate in the post-incision stages of the NER reaction (Coverley et al, 1992;Shivji et al, 1992) Serizawa et al, 1993a,b) and a helicase activity (Schaeffer et al, 1993 (Gerard et al, 1991).…”

Mismatch Repair during Homologous and Homeologous Recombination

“…Because it is likely that the ends of the resected DSB will not be complementary, virtually every SSA event will almost certainly require a flap-processing step. The 3 0 -protruding end removal in D-loop and SSA intermediates has been extensively studied (Schiestl and Prakash 1990;Fishman-Lobell et al 1992;Tomkinson et al 1993). The process appears to depend primarily on the ScMsh2-ScMsh3 heterodimer, which both recognizes and stabilizes flap-containing heteroduplexes Sugawara et al 1997;Kearney et al 2001).…”

Nuclear oscillations and nuclear filament formation accompany single-strand annealing repair of a dicentric chromosome in Saccharomyces cerevisiae

“…However, other studies of direct repeat recombination have shown that deletions commonly occur without generating the circular product predicted to form through a crossover event (Fishman-Lobell et al, 1992). Moreover, many deletion events are dependent on RAD1 and RAD10 (Klein, 1988;Schiestl and Prakash, 1990;Ivanov and Haber, 1995). The Rad1 and Rad10 proteins form a structurespecific endonuclease that generates 5′ single-stranded ends (Siede et al, 1993).…”