Radiation Induced Mutation for Resistance against Dry Root Rot in Chickpea (Cicer arietinum Linn.)

Abstract

Chickpea (Cicer arietinum L.) is an important cool-season food legume grown extensively by the poor farmers throughout the Indian subcontinent. In India chickpea is being grown in 8.32 million hectare with production of 9.8 million tones and 925 -kg/ha productivity (Project coordinators report, 2014-15). The Dry root rot (DRR) of chickpea caused by necrotropic fungus Rhizoctonia bataticola. During the past few decades, modern techniques such as mutation breeding by radiation and chemical mutagens and genetic engineering methodology have been tried to develop resistant cultivars of many crop plants. The utilization of mutation breeding is a simple, less cost full and time saving method. Present investigation entitled "Radiation induced mutation for resistance against Rhizoctonia bataticola in chickpea (Cicer arietinum Linn.)" was aimed at identification of suitable mutant or a combination of mutants influencing resistance to dry root rot in chickpea. The experimental material was consisted of the population of three selected cultivars of chickpea (JG 63, JG 74, and JG 130) grown in randomized complete block design in the Seed Breeding Farm, Department of Plant Breeding and Genetics, College of Agriculture, Jabalpur (M.P) under AICRP on chickpea project during Rabi 2014-15. Dry seeds (10-12% moisture content) of these varieties were irradiated with different doses of gamma rays (150Gy, 200Gy and 400 GY). Another set of presoaked seeds in distilled water (12hrs.) were treated with ethyl methane sulphonate at different concentration (0.3, 0.4 and 0.5%) prepared for 6 hrs. A portion of seeds irradiated at 150 and 200 GY gammaray doses were also treated with 0.3% and 0.4% EMS independently for 6 hrs. present findings revealed that JG 63, JG 74, JG 130 showed significant reaction for mutagenic treatments i.e, 200Gy, 400Gy, 0.3% EMS, 0.4% EMS, 150Gy+0.3% EMS, 200Gy+0.3% EMS. Among 11 treatments, 7 have shown effect on biological traits of experimental genotypes i.e. change in seedling height, decrease in germination percentage and decrease in plant height as compared to control. K e y w o r d sRhizoctonia bataticola, Gammaray, EMS, Cicer arietinum L

Radiation induced mutation for resistance/ tolerance against Fusarium oxysporum f. sp. ciceri in chickpea (Cicer arietinum L.)

“…Pusa-212 and Pusa-372, whereas it decreased beyond the dose of 300 Gy in the var. Pusa-212.It was further confirmed by comparing with similar results were earlier reported by Wani and Anis (2014) [10] , Pawar et al (2018) [4] , Ugandhar et al (2018) [7] .…”

Mutation induced alterations in agronomic traits of M1 generation chickpea

“…al., 1995) [7] . The utilization of mutation breeding is a simple, less cost full and time saving method proved in many cases to be useful in breeding new lines (Chobe et al, 2016, 2017, Pawar et al, 2018 [2,3,9] The aim of the present investigation is to use gamma rays and EMS as a nuclear technique to induce mutation for identifying response of mutagenic treatments on agronomical traits of M 1 generation mutant chickpea.…”