Radical Scavenging Activity of Ethanol Extracts and Solvent Partitioned Fractions from Various Red Seaweeds

조명래,; 이동진,; 유상권,

doi:10.4217/opr.2012.34.4.445

Cited by 15 publications

(5 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Therefore, in this study we investigated the antioxidant, anti-inflammatory, and antibacterial activity of a 70% EtOH extract of S. linearis. S. linearis extract exhibited weaker antioxidant activity (considering both DPPH and ABTS) than that previously reported the IC 50 value for DPPH of S. latiuscula MeOH extract was 9.03 μg/mL and the ABTS radical scavenging effect of S. latiuscula EtOH extract was 3.8% at 60 μg/mL (Cho et al, 2012;Park et al, 1998). Additionally, inhibition of ROS production by the S. linearis extract (even when assayed at a high concentration of 100 μg/mL) was ≤ 50% (Fig.…”

Section: Discussionmentioning

confidence: 63%

Antioxidant, anti-inflammatory, and antibacterial activities of a 70% ethanol-Symphyocladia linearis extract

Lee¹,

Yim²,

Kim³

et al. 2022

Fish Aquat Sci

View full text Add to dashboard Cite

Research on the potential biological activity of red alga Symphyocladia spp. has been limited to Symphyocladia latiuscula, which is widely used as a food ingredient in Korea. Here, we examined the biological activity of another species, Symphyocladia linearis, which is found in Korea and was reported as a new species in 2013. The aim of this study was to evaluate the antioxidant, anti-inflammatory, and antibacterial properties of a 70% ethanol extract of S. linearis. Antioxidant activity, which was evaluated using radical scavenging assays, revealed half maximal inhibitory concentration values for 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) and 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) of 34.57 and 11.70 μg/mL algal extract, respectively. Anti-inflammatory activity of the S. linearis ethanolic extract was evaluated using RAW 264.7 cells by measuring the inhibition of lipopolysaccharide-induced nitric oxide (NO) and prostaglandin E 2 (PGE 2 ) production. The potential cytotoxicity of NO and PGE 2 was first examined, confirming no toxicity at concentrations ranging from 10-100 μg/mL. NO production was inhibited 61.1% and 78.0% at 50 and 100 μg/mL S. linearis extract, respectively; and PGE 2 production was inhibited 69.1%, 83.2%, and 94.8% at 25, 50, and 100 μg/mL S. linearis extract, respectively. Thus, the S. linearis extract showed very strong efficacy against PGE 2 production. The cellular production of reactive oxygen species, measured using 2' ,7'-dichlorofluorescin diacetate fluorescence, was inhibited 48.8% by the addition of 100 μg/mL S. linearis extract. Antibacterial activity was evaluated using the disc diffusion method and minimum inhibitory concentration (MIC). S. linearis was effective only against gram-positive bacteria, exhibiting antibacterial activity against Staphylococcus aureus with a MIC of 256 μg/mL extract and against Bacillus cereus with a MIC of 1,024 μg/mL extract. Based on these results, we infer that a 70% ethanolic extract of S. linearis possesses strong anti-inflammatory properties, and therefore has the potential to be used in the prevention and treatment of inflammatory and immune diseases.

show abstract

Section: Discussionmentioning

confidence: 63%

Antioxidant, anti-inflammatory, and antibacterial activities of a 70% ethanol-Symphyocladia linearis extract

Lee¹,

Yim²,

Kim³

et al. 2022

Fish Aquat Sci

View full text Add to dashboard Cite

show abstract

“…Reactive oxygen species (ROS), including hydroxyl radicals, hydrogen peroxide, and superoxide anions, which are overexpressed by oxidative stress in the human body damage DNA, cell membranes, and proteins and enzymes within cells, in addition to inducing other harms, such as cell aging, an increase in cancer cells, and brain damage [ 30 , 31 ]. Thus, the analysis methods of ABTS and DPPH radical scavenging activities are typically utilized to determine how well a food ingredient with high antioxidant activity removes ROS [ 30 , 31 ]. The activity is measured by utilizing the principle in which a radical is reduced due to an antioxidant, resulting in decolorization from its intrinsic color [ 30 , 31 ].…”

Section: Resultsmentioning

confidence: 99%

“…Thus, the analysis methods of ABTS and DPPH radical scavenging activities are typically utilized to determine how well a food ingredient with high antioxidant activity removes ROS [ 30 , 31 ]. The activity is measured by utilizing the principle in which a radical is reduced due to an antioxidant, resulting in decolorization from its intrinsic color [ 30 , 31 ].…”

Section: Resultsmentioning

confidence: 99%

What Is the Relationship between Antioxidant Efficacy, Functional Composition, and Genetic Characteristics in Comparing Soybean Resources by Year?

et al. 2022

View full text Add to dashboard Cite

The aim of this study was to analyze the physiological activity of 48 soybean resources harvested in 2020 to identify the soybean resources’ relationships with individual isoflavone compounds and their genetic properties. These data will subsequently be compared with the research results on soybeans harvested in 2019. Initially, with respect to the physiological activity (6 types) and substances (19 types), this study evaluated the differences between the cultivation year (two years), seed coat color (three colors), and the interaction of the year and seed coat color of soybeans through ANOVA. Among the physiological activities, there were differences in the estrogen, estrogen receptor alpha, and UCP-1 (uncoupling protein-1) activities depending on the cultivation year. Moreover, there were differences in NO (nitric oxide), revealing differences in the ABTS (2, 2′-azino-bis-3ethylbenzo-thiazoline-6-sulfonic acid) and DPPH (2, 2-diphenyl-2-picrylhydrazyl) radical scavenging activities due to the seed coat color and the interaction of the year and seed coat color. Soybeans harvested in 2020 exhibited increased ABTS, DPPH, and NO inhibitory activities and reduced estrogen, estrogen receptor alpha, and UCP-1 activities compared to those harvested in 2019. According to the ANOVA results, eight of the nineteen individual derivatives illustrated yearly differences, while three derivatives displayed differences due to the seed coat color. Secondly, according to the relationship between the efficacy, derivative substances, and genetic properties, it was determined that genistein 7-O-(2″-O-apiosyl)glucoside (F5) is the individual isoflavone derivative that affected the six types of physiological activity, on which the genome-wide association study (GWAS) showed no significant differences for genetic properties. These results were inconsistent with the 2019 data, where three types of individual compounds, including F5, were proposed as substances that correlated with efficacy and there was a high correlation with genetic properties. Therefore, this study selected B17, B23, B15, B24, and Y7 as excellent varieties that are stable and highly functional in the cultivation environment, producing only small annual differences. The results of this study will be utilized as basic data for predicting soybean varieties and their cultivation, which have high environmental stability under climate variation and properly retain the functional substances and efficacy.

show abstract

“…이때 과도하게 생산된 활성산소는 위에 기술한 대로 세포의 노화를 유도한다 (Checa & Aran, 2020). 이러한 노화를 염증성 노화(inflammaging)로 분류한다 (Minciullo et al, 2016 (Cho et al, 2012;Yu & Lee, 2022). 한편 물로 추출한 경우에도 항 산화 및 항균, 항염 효과를 나타내어 화장품 원료로서 사용 가능성이 높다 (Lee & Ahn, 2022;Choi, 2022)…”

unclassified

“…. 홍조류의 추출 조건에 따른 항산화능을 비교한 선행 연구에서 나타난 결과와 유사하게 수성 추출이 에탄올 추출에 비해 높은 항산화능을 보여줌을 확인할 수 있었다(Cho et al, 2012).…”

unclassified

A Study on the Antioxidative and Anti-inflammatory Properties of Gloiopeltis furcata Extract Obtained Using Different Concentrations of Ethanol

Kim¹,

Lee²

2023

Asian J Beauty Cosmetol

View full text Add to dashboard Cite

Purpose: This study aimed to explore the differences in the antioxidant activity and anti-inflammatory effects of Gloiopeltis furcata based on its extraction conditions.Methods: 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) assays were performed to determine the antioxidant activity of Gloiopeltis furcata extracts obtained using different concentrations of ethanol. Concomitantly, the concentrations of polyphenols and flavonoids, which are antioxidants present in Gloiopeltis furcata extract, were measured. Additionally, in cell-based experiments, the cytotoxicity and anti-inflammatory activity of a water extract, which exhibited the most potent antioxidative activity among all extracts, was measured.Results: In the DPPH assay, the Gloiopeltis furcata extract exhibited an antioxidant activity of 3.576 mg of ascorbic acid/g, whereas in the ABTS assay, the antioxidant activity was 4.892 mg of ascorbic acid/g. The polyphenol concentration was found to be 35.52±2.32 mg/g, whereas the flavonoid concentration was 0.88±0.34 mg/g. Furthermore, the extract had a cytotoxicity of ≤20% at all concentrations used in the experiment, thus indicating the absence of cytotoxicity and confirming that the extract was effective in reducing inflammation proportionally to its concentration. Moreover, the extract exhibited an anti-inflammatory activity of 47.66%±1.53% at a concentration of 100 μg/mL.Conclusion: Gloiopeltis furcata exhibited the most potent antioxidant activity in its water extract, and it was confirmed that the extract of Gloiopeltis furcata that was obtained through water had low cytotoxicity, an anti-inflammatory effect, and a commercial value.

show abstract

Radical Scavenging Activity of Ethanol Extracts and Solvent Partitioned Fractions from Various Red Seaweeds

Cited by 15 publications

References 24 publications

Antioxidant, anti-inflammatory, and antibacterial activities of a 70% ethanol-Symphyocladia linearis extract

Antioxidant, anti-inflammatory, and antibacterial activities of a 70% ethanol-Symphyocladia linearis extract

What Is the Relationship between Antioxidant Efficacy, Functional Composition, and Genetic Characteristics in Comparing Soybean Resources by Year?

A Study on the Antioxidative and Anti-inflammatory Properties of Gloiopeltis furcata Extract Obtained Using Different Concentrations of Ethanol

Contact Info

Product

Resources

About