Radical Scavenging Activity of Polyphenols in Young Leaves of Sesamum indicum L.

Matsufuji, Hiroshi; Ohmori, J.; Goto, Shuichi; Chino, Makoto; Wada, Etsuji; Uchida, Ayumi; Fukahori, Katsunori; Yamagata, Kazuo; Sakurai, Hiroyuki

doi:10.3136/nskkk.58.88

Cited by 6 publications

(9 citation statements)

References 25 publications

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“…DPPH assays were performed according to our previous report [ 12 ] with modifications to allow the use of 96-well plates. Authentic and isolated compounds were dissolved in 60% MeOH (except P4 , which was dissolved in DMSO) and diluted with 60% MeOH as necessary.…”

Section: Methodsmentioning

confidence: 99%

“… a Our previous report [ 12 ]. b The value in box brackets shows signal data in α-forms of P1 -gluose moiety.…”

Section: Supporting Informationmentioning

confidence: 99%

“…indicum leaves (0.5–2.6 μg/g dry weight; 0.00005–0.00026%). We found that young sesame leaves contain more than eight polyphenols, with acteoside ( P5 in this study), a phenylethanoid, being one of the major components [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

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Chemical characterization and biological activity in young sesame leaves (Sesamum indicum L.) and changes in iridoid and polyphenol content at different growth stages

Fuji

Uchida²,

Fukahori³

et al. 2018

PLoS ONE

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Three iridoids (lamalbid (I1), sesamoside (I2) and shanzhiside methyl ester (I3)) and seven polyphenols (cistanoside F (P1), chlorogenic acid (P2), pedalitin-6-O-laminaribioside (P3), pedaliin (P4), isoacteoside (P6), pedalitin (P7) and martynoside (P8)) were identified in young sesame leaves (Sesamum indicum L.) other than the acteoside (P5) reported previously. P3 was a new compound, and I1, I3, P2 and P8 were found in a species of Sesamum for the first time. HPLC analyses revealed that the compounds I1 (0.29–1.75% of dry leaves), I2 (0.38–0.87%), I3 (0.04–1.07%), P4 (0.01–2.05%) and P5 (0.13–4.86%) were present primarily in young sesame leaves and were found in plants cultivated on different farms (plant height, 30–70 cm). Of the identified compounds, P5 and P6 showed high 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging, oxygen radical absorbance capacity (ORAC), and in vitro antiglycation activities. Given its content, P5 makes a major contribution to the biological activities of young sesame leaves. The compounds were examined at six different growth stages of plants cultured in a greenhouse to determine the optimum harvest stage and for end-use assessment. P5 accumulated in the leaves during growth, and the content reached a maximum of 12.9% of dry leaves in the 4th stage (plant height, 74.5±9.7 cm), which is one of the highest percentages reported in plants from nature.

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Section: Methodsmentioning

confidence: 99%

“… a Our previous report [ 12 ]. b The value in box brackets shows signal data in α-forms of P1 -gluose moiety.…”

Section: Supporting Informationmentioning

confidence: 99%

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Chemical characterization and biological activity in young sesame leaves (Sesamum indicum L.) and changes in iridoid and polyphenol content at different growth stages

Fuji

Uchida²,

Fukahori³

et al. 2018

PLoS ONE

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“…Kubmarawa et al [ 25 ] reported that dry leaf of sesame is comprised approximately of 18.59 % protein, 34.04 % carbohydrate, 1.66 % oil, low tannins and phytates, and seventeen amino acids, including seven essentials. Morita [ 26 ] has detected pedaliin and pedalitin in sesame leaves, and Matsufuji et al [ 20 ], acteoside and verbascoside. In addition, Dat et al [ 18 ], have detected 3-epibartogenic acid, epigallocatechin, and a kaempferol derivative, and Sarma et al [ 19 ], gallic acid, chlorogenic acid, and quercetin.…”

Section: Introductionmentioning

confidence: 99%

Comparative metabolomics analysis of different sesame (Sesamum indicum L.) tissues reveals a tissue-specific accumulation of metabolites

Dossou

Cui

et al. 2021

BMC Plant Biol

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Background Sesame (Sesamum indicum L.) leaves, flowers, especially seeds are used in traditional medicine to prevent or cure various diseases. Its seed’s market is expanding. However, the other tissues are still underexploited due to the lack of information related to metabolites distribution and variability in the plant. Herein, the metabolite profiles of five sesame tissues (leaves, fresh seeds, white and purple flowers, and fresh carpels) have been investigated using ultra-high-performance liquid chromatography-mass spectrometry (UPLC-MS/MS)-based widely targeted metabolomics analysis platform. Results In total, 776 metabolites belonging to diverse classes were qualitatively and quantitatively identified. The different tissues exhibited obvious differences in metabolites composition. The majority of flavonoids predominantly accumulated in flowers. Amino acids and derivatives, and lipids were identified predominantly in fresh seeds followed by flowers. Many metabolites, including quinones, coumarins, tannins, vitamins, terpenoids and some bioactive phenolic acids (acteoside, isoacteoside, verbascoside, plantamajoside, etc.) accumulated mostly in leaves. Lignans were principally detected in seeds. 238 key significantly differential metabolites were filtered out. KEGG annotation and enrichment analyses of the differential metabolites revealed that flavonoid biosynthesis, amino acids biosynthesis, and phenylpropanoid biosynthesis were the main differently regulated pathways. In addition to the tissue-specific accumulation of metabolites, we noticed a cooperative relationship between leaves, fresh carpels, and developing seeds in terms of metabolites transfer. Delphinidin-3-O-(6ʺ-O-p-coumaroyl)glucoside and most of the flavonols were up-regulated in the purple flowers indicating they might be responsible for the purple coloration. Conclusion This study revealed that the metabolic processes in the sesame tissues are differently regulated. It offers valuable resources for investigating gene-metabolites interactions in sesame tissues and examining metabolic transports during seed development in sesame. Furthermore, our findings provide crucial knowledge that will facilitate sesame biomass valorization.

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“…In previous studies, our group chemically characterized three iridoids and eight polyphenols in young sesame leaves (Sesamum indicum L.), including their biological activities such as 1,1-diphenyl-2-picrylhydrazyl radical scavenging, oxygen radical absorbance capacity, in vitro antiglycation activity, changes in the content of five compounds at each growth stage of sesame leaves, and the accumulation and subcellular localization of acteoside (verbascoside) in sesame plants [12][13][14]. Among the polyphenols identified, acteoside and pedaliin (pedalitin-6-O-glucoside) are respectively a major phenylethanol glycoside and a flavonoid glycoside in young sesame leaves.…”

Section: Introductionmentioning

confidence: 99%

Development of an HPLC method with relative molar sensitivity based on 1H-qNMR to determine acteoside and pedaliin in dried sesame leaf powders and processed foods

et al. 2020

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A high-performance liquid chromatography (HPLC) method with relative molar sensitivity (RMS) based on 1H quantitative NMR spectroscopy (1H-qNMR) has been developed for food ingredients such as acteoside (verbascoside) and pedaliin (pedalitin-6-O-glucoside) without requiring authentic and identical standards as the reliable analytical methods. This method is used methyl 4-hydroxybenzoate (MHB) as an alternative reference standard. Each RMS is also calculated from the ratio of each analyte's molar absorption coefficient to that of MHB after correcting the purities of the analytes and reference standard by 1H-qNMR. Therefore, this method can quantify several analytes with metrological traceability to the International System of Units (SI) using the RMS and one alternative reference standard. In this study, the content of acteoside and pedaliin in several samples, such as dried sesame leaf powders and commercially processed foods, can be determined by the proposed RMS method and demonstrated in good agreement that obtained by a conventional method. Moreover, the proposed method yields analytical data with SI-traceability without the need for an authentic and identical analyte standard. Thus, the proposed RMS method is a useful and practical tool for determining acteoside and pedaliin in terms of the accuracy of quantitative values, the routine analysis, and the cost of reagents.

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Radical Scavenging Activity of Polyphenols in Young Leaves of Sesamum indicum L.

Cited by 6 publications

References 25 publications

Chemical characterization and biological activity in young sesame leaves (Sesamum indicum L.) and changes in iridoid and polyphenol content at different growth stages

Chemical characterization and biological activity in young sesame leaves (Sesamum indicum L.) and changes in iridoid and polyphenol content at different growth stages

Comparative metabolomics analysis of different sesame (Sesamum indicum L.) tissues reveals a tissue-specific accumulation of metabolites

Development of an HPLC method with relative molar sensitivity based on 1H-qNMR to determine acteoside and pedaliin in dried sesame leaf powders and processed foods

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