Radical Scavenging and Antimicrobial Properties of Polyphenol Rich Waste Wood Extracts

Abstract

The main focus of this study is to assess radical scavenging and antimicrobial activities of the 11 wood extracts: oak (Quercus petraea (Matt.) Liebl., Q. robur L., and Q. cerris L.), mulberry (Morus alba L.), myrobalan plum (Prunus cerasifera Ehrh.), black locust (Robinia pseudoacacia L.), and wild cherry (Prunus avium L.). High-performance thin-layer chromatography (HPTLC) provided initial phenolic screening and revealed different chemical patterns among investigated wood extracts. To identify individual compounds with radical scavenging activity DPPH-HPTLC, assay was applied. Gallic acid, ferulic and/or caffeic acids were identified as the compounds with the highest contribution of total radical scavenging activity. Principal component analysis was applied on the data set obtained from HPTLC chromatogram to classify samples based on chemical fingerprints: Quercus spp. formed separate clusters from the other wood samples. The wood extracts were evaluated for their antimicrobial activity against eight representative human and opportunistic pathogens. The lowest minimum inhibitory concentration (MIC) was recorded against Staphylococcus aureus for black locust, cherry and mulberry wood extracts. This work provided simple, low-cost and high-throughput screening of phenolic compounds and assessments of the radical scavenging properties of selected individual metabolites from natural matrix that contributed to scavenge free radicals.

A Strategy to Revalue a Wood Waste for Simultaneous Cadmium Removal and Wastewater Disinfection

“…Namely, the consumption of bacteriologically unsafe drinking water is still one of the most significant causes of morbidity and mortality [19], especially in rural areas. Smailagić et al [20] investigated the antimicrobial activity of eleven wood extracts (oaks, mulberry, myrobalan plum, black locust, and wild cherry) against eight representative human and opportunistic pathogens. The lowest minimum inhibitory concentration (MIC) was observed against S. aureus for black locust, cherry, and mulberry wood extracts, and based on the conducted research, the usefulness of such wood waste for water disinfection is expected.…”

Influence of extraction time, solvent and wood specie on experimentally aged spirits – A simple tool to differentiate wood species used in cooperage

“…According to Alañón, Castro-Vázquez, Díaz-Maroto, Hermosín Gutiérrez, Gordon & Pérez -Coello (2011), the major contributors to the antioxidant capacity in wood samples are phenolic acids, such as gallic, protocatechuic, p-coumaric, and ellagic acid, and all the ellagitannins. Further, we reported that gallic acid, ferulic acid, p-coumaric acid, and caffeic acid, present in such samples, significantly contributed to the antioxidant capacity (Smailagić et al, 2020). All these compounds showed similar electrochemical behavior in the testing experimental conditions (Simić, Manojlović, Š egan & Todorović, 2007) with the corresponding oxidation peaks at similar potential.…”

“…So far, we have investigated the content of individual polyphenols by liquid chromatography with mass spectrometric detection, while spectrophotometric methods were used for conducting total phenolic content and antioxidant capacity (Smailagić et al, 2019). Also, individual compounds with radical scavenging activity were investigated using DPPH-HPTLC assay (Smailagić et al, 2020). Also, our research group investigated potential application of the electrochemical methods in the monitoring of the various biochemical or biological processes that affected the antioxidant capacity of the dominantly food samples.…”

Antimicrobial Activity of Water-Ethanolic Extractions From Quercus Robur L. Leaves and Buds

“…Официнальным классическим лекарственным средством, обладающим вяжущими и противовоспалительными свойствами, является отвар коры дуба черешчатого [5][6][7]. За последнее время было проведено большое количество исследований, посвященных изучению антимикробных свойств коры дуба, а также препаратов на ее основе [8][9][10][11][12][13][14][15][16]. Кора дуба черешчатого входит в состав многих комплексных препаратов, например, «Стоматофит», «Тонзилгон Н», «Дентос» и др., а также используется для получения экстрактов, используемых в медицинских и косметологических целях 1 .…”