Radio-synthesized protein-based nanoparticles for biomedical purposes

Varca, Gustavo H.C.; Ferraz, Caroline C.; Lopes, Patrícia Santos; Mathor, Mônica Beatriz; Grasselli, Mariano; Lugão, Ademar B.

doi:10.1016/j.radphyschem.2013.05.057

Cited by 21 publications

(13 citation statements)

References 18 publications

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“…This process requires organic solvents and the removal of both the surfactants and oily residues is problematic. Moreover, the thermal gelation [9], the nanospray drying [10], the gamma irradiation [11], the albuminbound technology (nab-technology) [12] or the self-assembly [13] techniques are also known for fabrication of different drugcontaining albumin NPs. Comparing the mentioned techniques, we can conclude that the thermal stabilization is only suitable for encapsulating drug molecules that are not heat sensitive.…”

Section: Introductionmentioning

confidence: 99%

Novel feasibilities for preparation of serum albumin-based core-shell nanoparticles in flow conditions

et al. 2020

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We first demonstrate a simple and rapid fabrication protocol of bovine serum albumin (BSA) nanoparticles (NPs), as potential drug carriers using a microchannel flow technique with the successful encapsulation of a water-soluble kynurenic acid (KYNA) having neuroactive property has also been performed. By comparison, the preparation of a hydrophobic α-Tocopherol (TP)-loaded polylactide-co-glycolide 50:50 (PLGA50)-based NPs was also carried out under flow conditions. We highlight several benefits of the flow technique over the commonly known self-assembly and nanoprecipitation processes. The average particle diameter, the size distribution, the encapsulation efficiency (EE%) and the drug release kinetics of these different core-shell type NPs prepared by the flow as well as the above-mentioned classic methods were compared. The decisive role of the flow rate (FR), the relative flow rate (RFR) of the components in the particle size of both BSA-and PLGA50-based NPs have also been verified. By utilization of the optimal flow conditions, the average size can be decreased with ca. 15-20% and lower polydispersity index (PDI) can be also achieved. It was confirmed that the systematic change of the RFR values resulting in the controlled size of the drug-loaded BSA NPs between 120 and 140 nm, while d = 149 nm was obtained for self-assembled NPs. However, for BSA-based NPs quite similar EE% was obtained for both methods (ca. 11-12%), but for PLGA50/TP NPs the application of flow device increased the EE% from 67.0% to 71.5%.

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Section: Introductionmentioning

confidence: 99%

Novel feasibilities for preparation of serum albumin-based core-shell nanoparticles in flow conditions

et al. 2020

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“…The development of enzyme nanoparticles represents an alternative to overcome such problems [11]. For instance, papain nanoparticles previously reported by Varca and co-workers [12][13][14] were hypothesized to have better biopharmaceutical properties-such as cell-specific targeting and improved drug loading capacity-than their bulk counterparts and may be a possible solution for cosmetic or pharmaceutical formulations.…”

Section: Introductionmentioning

confidence: 99%

Semi-Solid Pharmaceutical Formulations for the Delivery of Papain Nanoparticles

et al. 2020

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Papain is a therapeutic enzyme with restricted applications due to associated allergenic reactions. Papain nanoparticles have shown to be safe for biomedical use, although a method for proper drug loading and release remains to be developed. Thus, the objective of this work was to develop and assess the stability of papain nanoparticles in a prototype semi-solid formulation suitable for dermatological or topical administrations. Papain nanoparticles of 7.0 ± 0.1 nm were synthesized and loaded into carboxymethylcellulose- and poly(vinyl alcohol)-based gels. The formulations were then assayed for preliminary stability, enzyme activity, cytotoxicity studies, and characterized according to their microstructures and protein distribution. The formulations were suitable for papain nanoparticle loading and provided a stable environment for the nanoparticles. The enzyme distribution along the gel matrix was homogeneous for all the formulations, and the proteolytic activity was preserved after the gel preparation. Both gels presented a slow release of the papain nanoparticles for four days. Cell viability assays revealed no potential cytotoxicity, and the presence of the nanoparticles did not alter the microstructure of the gel. The developed systems presented a potential for biomedical applications, either as drug delivery systems for papain nanoparticles and/or its complexes.

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“…propriedades antitumorais e grande perspectiva para carreamento de fármacos (Varca et al, 2014a;Muller et al, 2016) Muller e colaboradores 2016 A exposição das proteínas a essas espécies provoca mudanças conformacionais (Giulivi et al, 2003) (Giulivi et al, 2003).…”

Section: Enzima Proteolítica De Interesse Biotecnológicounclassified

“…Além disso, podem ser sintetizadas por várias rotas, entre elas a preparação de complexos em solução aquosa (Muller et al, 2013), por radiação utilizando 60 Co como fonte radioativa (Varca et al, 2014a) ou por feixe de elétron (electron beam), (Varca et al, 2016) e tendem a não apresentar toxicidade significativa (Tarhini et al, 2017).…”

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Síntese induzida por radiação de nanocarreadores bioativos à base de papaína para carreamento de radiofármaco

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Aos meus pais, Ivone e Ismael, que investiram no meu estudo, incentivaram e me instruiram a ser quem sou hoje. Obrigada por todas as broncas e conselhos, pois me fizeram crescer e perseverar. Ao meu companheiro, Felipe, por toda a paciência e dedicação diária. Por acreditar e me inspirar todos os dias. Obrigada por tudo! Ao meu irmão, Matheus, pelo carinho e parceria. Muito obrigada por me acompanhar nesta vida. As melhores amigas Angel, Natália e Tássia, que nas horas mais difíceis estavam presentes para me apoiar. Obrigada por todos os momentos que compartilhamos! Aos meus afilhados, Arthur, Helena e Giovanna, por alegrarem meus dias com sorrisos, brincadeiras e tanto carinho. A Katia, Ilson, Andresa e Elton, por me receberam de braços abertos e compartilhar tantos momentos juntos. Ao meu orientador Prof. Dr. Ademar Lugão pelo conhecimento transmitido e por acreditar no meu potencial. Aos amigos Justine, Gustavo Varca e a pequena Lara, que estiveram ao meu lado nas horas fáceis e difíceis e me ensinaram o valor de uma grande amizade. Muito obrigada por acreditarem em mim e me ensinarem tanto! Gu, obrigada também pela co-orientação. Você foi fundamental no meu crescimento. Aos meus queridos Lucas Freitas de Freitas e Caroline Lima, pela parceria, amizade e suporte. Agradeço todo dia por terem aparecido na minha vida. Sem vocês os meus dias não seriam tão felizes! Aos amigos do laboratório Nanothera, Kamila Nogueira, Thomaz Sevilha, Jorge Gabriel, Francoleno Avelino, Cássia Priscila, Aryel Heitor. Pelas conquistas compartilhadas. Pela força e atenção. Pela amizade. A Bruna Roque, pela amizade, conversas e por me ajudar tantas vezes que precisei. Aos professores e pesquisadores que fizeram parte da minha trajetória, Dr. Fábio Marques, pelo suporte e conhecimento passado na etapa de radiomarcação, Dr. Sławomir Kadłubowski pela ajuda nos artigos e parceria. À Elizabeth Somessari, Paulo Santos e Dr. Pablo Vásquez pela irradiação das amostras. Ao João e ao Takeshi que estiveram sempre a disposição para ajudar nos serviços de manutenção. Pelas conversas e cafés da manhã.

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Radio-synthesized protein-based nanoparticles for biomedical purposes

Cited by 21 publications

References 18 publications

Novel feasibilities for preparation of serum albumin-based core-shell nanoparticles in flow conditions

Novel feasibilities for preparation of serum albumin-based core-shell nanoparticles in flow conditions

Semi-Solid Pharmaceutical Formulations for the Delivery of Papain Nanoparticles

Síntese induzida por radiação de nanocarreadores bioativos à base de papaína para carreamento de radiofármaco

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