Radioautographic observations on DNA-synthesis in the human placenta under normal and pathologic conditions

Cellular Proliferation in Villi of Normal and Pathological Pregnancies

“…This finding is quite in keeping with earlier state ments of Kallenbach et al [14] and Geier et al [7], Our figures are somewhat more ele vated than those of these authors (table I). It must be noted however that our incubation time was 4 h as opposed to the 1 h h of Kaltenbach et al [14] and 1 h of Geier et al [7].…”

“…This finding is quite in keeping with earlier state ments of Kallenbach et al [14] and Geier et al [7], Our figures are somewhat more ele vated than those of these authors (table I). It must be noted however that our incubation time was 4 h as opposed to the 1 h h of Kaltenbach et al [14] and 1 h of Geier et al [7]. It has been shown [8][9][10][11][12][13][14][15][16] that the average S (synthesis) phase for first and second trimes ter cytotrophoblast lasted 5-6 h. Obviously by increasing considerably our incubation time, we have caught more cells entering the S phase.…”

“…Isolated IUGR in normotensive patients did not dif fer in this respect from control cases. Such a finding was already mentioned by Kaltenbach et al [14], It seems logical to postulate that the increase of 3H-thymidine incorpora tion in cytotrophoblast reflects a reparation or replacement process. It is well known that syncytiotrophoblast is easily damaged in preeclampsia [4,13] or in hypoxic states [5] and results in abundant syncytial sprout for mation followed by knot detachment into maternal circulation [11].…”