Radioimmune assay of carcinoembryonic antigen

Egan, Marianne L.; Lauenschleger, J.T.; Coligan, John E.; Todd, Charles W.

doi:10.1016/0019-2791(72)90093-6

Cited by 168 publications

(23 citation statements)

References 13 publications

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“…The assay method employed was the double antibody technique of nonequilibrium system, modified from the methods of Egan et al (1972) and Laurence et al (1972b). One hundred ,ul of serum sample was mixed with 100 ,ul of 5% normal rabbit serum in phosphate-buffered saline (PBS) at pH 7-4 and then with 100 ,ul of anti-CEA * Reprints may be requested from: Satoshi Hamada, M.D., Radioisotope Research Centre, Kyoto University, Kyoto 606, Japan. antiserum diluted 1: 12,000.…”

Section: Radioimmunoassaymentioning

confidence: 99%

Association of medullary carcinoma of the thyroid with carcinoembryonic antigen

Ishikawa¹,

Hamada²

1976

Br J Cancer

113

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Summary.-To investigate the association between medullary carcinoma of the thyroid (MCT) and carcinoembryonic antigen (CEA), we assayed 78 sera from patients with thyroid diseases for CEA, employing the radioimmunoassay of double antibody technique. All 13 sera from patients with MCT had high levels of CEA, ranging from 14 to 170 ng/ml. Increased serum CEA was noted .even in cases of small, localized carcinoma. By contrast, serum CEA levels were normal (below 10 ng/ml) in all other histological types of thyroid carcinoma (33 cases), except for one case of papillary adenocarcinoma. In 32 patients with non-malignant thyroid diseases, with few exceptions serum CEA levels remained within the normal range. The elevated serum levels of CEA in MCT returned to normal after successful operation. Furthermore, very high tissue concentrations of CEA were demonstrated in MCT. The results indicate that CEA is actively produced by MCT, and that its measurement is useful in the diagnosis and management of the disease. It is suggested that the highly specific association of CEA with MCT may well be related to a defect of neural crest origin.

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Section: Radioimmunoassaymentioning

confidence: 99%

Association of medullary carcinoma of the thyroid with carcinoembryonic antigen

Ishikawa¹,

Hamada²

1976

Br J Cancer

113

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“…The precise CEA levels, in numerical terms, will vary in accordance with the type of radioimmunoassay employed. Nonetheless, all the available methods seem to give largely comparable results (LoGerfo et al, 1971;Chu and Reynoso, 1972;Egan et al, 1972;Boenisch and Raam, 1975). In tumours the plasma levels are predominantly related to the extent and site of tumour spread or tumour volume (Laurence et al, have been clinically overt (Table 3).…”

mentioning

confidence: 99%

Biochemical Monitoring of Cancer: A Review

1976

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“…CEA wias measured by a double antibody radioimmunoassay method of Egan et al (1972) and modified by using a single label (1251 -CEA) as described by Laurence et al (1972). AFP was measured by a radioimmunoassay method as described by Leek and Chard (1974); highly purified AFP was used in this assay and polyethylene glycol used for separation of bound and free antigen.…”

Section: Methodsmentioning

confidence: 99%

Tumour-associated immune responses and isolated carcinoembryonic antigen and alpha feto-protein levels related to survival in ovarian cancer patients

Levin¹,

McHardy²,

Poulton³

et al. 1976

Br J Cancer

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Summary.-The presence of a tumour-associated immune response in 37 patients with ovarian cancer as assessed by blastogenesis (lymphocyte transformation) evoked by ovarian cancer cell extracts, has been correlated with survival following the test. The difference in these responses is unlikely to be accounted for on the basis of general impairment of cell-mediated immuno-competence.Serum carcinoembryonic antigen (CEA) was also determined in 27 ovarian cancer patients to assess its prognostic significance. Raised CEA levels and absence of blastogenic response to tumour cell extract during relapse are associated with a worse prognosis but neither of these parameters are significant in remission.Possible applications of these findings to the clinical management of ovarian cancer patients are discussed.Serum alpha feto-protein levels measured by radioimmunoassay were not found to be raised in any of the 32 ovarian cancer patients in whom it was measured.

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Radioimmune assay of carcinoembryonic antigen

Cited by 168 publications

References 13 publications

Association of medullary carcinoma of the thyroid with carcinoembryonic antigen

Association of medullary carcinoma of the thyroid with carcinoembryonic antigen

Biochemical Monitoring of Cancer: A Review

Tumour-associated immune responses and isolated carcinoembryonic antigen and alpha feto-protein levels related to survival in ovarian cancer patients

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