Radioimmunoassay for Plasma Antidiuretic Hormone

Johnston, C. I.

doi:10.1677/joe.0.0520069

Cited by 34 publications

(23 citation statements)

References 11 publications

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“…DISCUSSION The development of a clinically useful radioimmunoassay for plasma vasopressin has proven to be more difficult than for many of the other polypeptide hormones. Although a number of other laboratories have succeeded by a variety of methods in raising antisera to vasopressin (7)(8)(9)(10)(11)(12)(13)(14)(15), none has been sufficiently sensitive to detect the hormone at the low concentrations (10-M) normally present in plasma. Moreover, when carefully studied, several of these antisera have been found to be unusually susceptible to interference by a The effect of acute hypotension on plasma AVP in a healthy subject.…”

Section: Resultsmentioning

confidence: 99%

Development and Clinical Application of a New Method for the Radioimmunoassay of Arginine Vasopressin in Human Plasma

Robertson¹,

Mahr²,

Athar³

et al. 1973

J. Clin. Invest.

781

299

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Section: Resultsmentioning

confidence: 99%

Development and Clinical Application of a New Method for the Radioimmunoassay of Arginine Vasopressin in Human Plasma

Robertson¹,

Mahr²,

Athar³

et al. 1973

J. Clin. Invest.

781

299

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“…Initially a dose of arginine vasopressin (AVP) (Ferring1 mu 2-3 pmol) was calculated on the basis of work of Johnston [1972]. Assuming that the clearance rate per kilogram body weight was the same for adult and fetal sheep, a dose of 200 pmol .…”

Section: Methodsmentioning

confidence: 99%

The Effects of Antidiuretic Hormone on Urine Flow and Composition in the Chronically‐cannulated Ovine Fetus

et al. 1978

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The fetuses of nine pregnant ewes were chronically cannulated between 86 and 130 days with cannulae in one carotid artery, one jugular vein, the fetal bladder and the amniotic cavity. The effects of infused AVP on fetal urine flow rate and composition were studied. A dose of 35 pmol . h-' always caused an increase in urine osmolality and a decrease in flow rate and free water clearance without change in blood pressure. Higher doses (140 pmol.h-1) were significantly pressor and caused increased excretion of sodium, chloride, urea and creatinine. Although this dose always caused an increase in urine osmolality, in five experiments the flow rate also rose. It is concluded that the ovine fetus has the ability to control its own urine flow and composition, and possibly also amniotic fluid volume and composition.Although the compositions of ovine fetal urine and amniotic fluid have been studied by several groups of workers [Alexander, Nixon, Widdas and Wohlzogen 1958;Mellor and Slater, 1972], little is known of the mechanisms which control the volumes and compositions of these fluids. Urine formation begins at about the third month of fetal life in the human [Vernier and Smith, 1968] and by the third trimester is thought to be an important source of amniotic fluid. This view is supported by clinical findings that when urine production is impaired in some way, oligohydramnios often results [Jeffcoate and Scott, 1959]. Thus in studying the maintenance of amniotic fluid volume and composition it becomes relevant to investigate possible controlling factors of fetal renal function.Antidiuretic hormone (ADH) is a hormone which affects renal function in the adult. It has been shown to be present in ovine fetal blood [Alexander, Britton, Forsling, Nixon and Ratcliffe, 1974] but a physiological effect has not yet been demonstrated. In the experiments to be described in this paper a chronic preparation in pregnant sheep was used to study the effects of infused antidiuretic hormone on urine composition and flow rate in the ovine fetus. METHODS Surgery. The fetuses of nine cross-bred merino ewes of known mating dates had vascular, bladder and amniotic cannulae inserted at 86-130 days gestational age (term-147 ± 5 days).

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“…Blood pressure was measured in conscious rats by a tail plethysmographic method. Plasma renin was measured by a competitive antibody method employing radioimmunoassay for generated angiotensin I (Johnston et al, 1969 and1972) and plasma angiotensin II on extracted plasma by radioimmunoassay. Plasma vasopressin was measured at 28 days or longer after renal artery steuosis by radioimmunoassay (Johnston, 1972 ;Pnllan et al, 1977).…”

Section: Methodsmentioning

confidence: 99%

Vasoactive peptides in experimental renal hypertension

1978

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1. Blood pressure is controlled by both integrated neurogenic and humoral vasoactive mechanisms. 2. Both vasopressor (angiotensin and vasopressin) and vasodepressor (bradykinin) hormonal peptides have been identified. 3. In acute experimental renal hypertension in the rat plasma renin, angiotensin and vasopressin have all been shown to be elevated. 4. Associated with this increase in vasopressor hormonal peptides, urinary kallikrein excretion has been demonstrated to be reduced during the development of renal hypertension. 5. The level of blood pressure achieved in experimental renal hypertension is probably a summation of these vasoactive peptides as well as other factors.

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Radioimmunoassay for Plasma Antidiuretic Hormone

Cited by 34 publications

References 11 publications

Development and Clinical Application of a New Method for the Radioimmunoassay of Arginine Vasopressin in Human Plasma

Development and Clinical Application of a New Method for the Radioimmunoassay of Arginine Vasopressin in Human Plasma

The Effects of Antidiuretic Hormone on Urine Flow and Composition in the Chronically‐cannulated Ovine Fetus

Vasoactive peptides in experimental renal hypertension

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