Radioimmunoassay of Fibrinogen and Its Proteolysis Products

Kj, Catt; Hirsh, Jay; Dj, Castelan; Hd, Niall; Gw, Tregear

doi:10.1055/s-0038-1651243

Cited by 16 publications

(7 citation statements)

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“…The TRCHII is sensitive to early (X and Y) as well as late (D and E) products of fibrinogen digestion by plasmin. During digestion of fibrinogen the assay values increase at firstlater diminishing (Merskey et al 1966 ;Catt et al 1968). The experiment has now been repeated on AutoAnalyzer (figure 1).…”

Section: Merskeymentioning

confidence: 99%

Discussion 10–16

1971

Scandinavian Journal of Haematology

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Section: Merskeymentioning

confidence: 99%

Discussion 10–16

1971

Scandinavian Journal of Haematology

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“…By contrast, a radioimmunoassay should be considerably more sensitive and, since the endpoint is objective, more precise. Several groups have described radioimmunoassays for fibrinogen and its degradation products (Catt et al, 1968;Plow & Edgington, 1973) but only one has been found to have practical application (Gordon et al, 1973).…”

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confidence: 99%

The Development of Radioimmunoassays for Fibrinogen Degradation Products: Fragments D and E

et al. 1975

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Fibrinogen degradation products, fragment D (FgD) and fragment E (FgE) have been measured in human serum by specific radioimmunoassays. In addition, the appearance of a neoantigenic determinant on FgD, revealed when fibrinogen is degraded by plasmin has been utilized to develop a specific radioimmunoassay for FgD in plasma (FgDneo). The reagents and conditions used in each assay are described in detail. The mean specific activity was 144 muCi/mug for 125I-labelled FgE and 82 muCi/mug for 125I-labelled FgD. Separation of antibody bound and free antigen was achieved using second antibody. The detection limits of the FgE, FgD and FgDneo assays were 0.8, 1.0 and 6.2 ng/ml respectively. The specificity of each assay with respect to fibrinogen and its degradation fragments has been assessed. Fibrinogen and fragment X cross-reacted markedly in both the FgE and FgD assays, whereas the cross-reaction of fibrinogen was abolished in the FgDneo assay, while the cross-reaction of fragment X was 10%, indicating gradual emergence of the neoantigenic site during digestion of fibriogen. The sensitivity, precision, and specificity of the radioimmunoassay systems described have major advantages over the existing procedures for the measurement of fibrinogen degradation products.

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“…The presence of fibrinogeii/fibrin degradation products (FDP) in scruni has becii recognized since 1961 (Ferreira & Murat, 1961), and the measurement of FDP is now a well-accepted procedure in the diagnosis of both disseminated and local intravascular coagulation. A number of assays for FDP have been described, most of which are based on immunological techniques including flocculation (Ferreira & Murat, 1961), immuiiodiffusioli and immunoelectrophoresis (Nilkhn & Nilsson, 1964), tamed red cell liaeinagglutiiiatioii inhibition (Merskey et a/, 1966), latex particle agglutination (Allington, 1971) and radioimmunoassay (Catt et a/, 1968;Plow & Edgingtoii, 1973 ;Gordon et al, 1973).…”

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A Comparison between Radioimmunoassay and Other Immunological Techniques for the Measurement of Fibrinogen/Fibrin Degradation Products in Serum

et al. 1975

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Fibrinogen/fibrin degradation products (FDP) have been measured in serum by radioimmunoassay for fragment E (FgE), one of the terminal fragments in plasmic digestion of fibrinogen, and the results compared with those determined by both a tanned red cell haemagglutination inhibiton immunoassay (TRCHII) and a latex particle agglutination inhibition immunoassay. The detection limit of the FgE assay was 0.8 ng/ml, that of TRCHII was 625 ng/ml and the latex particle agglunitaion inhibition immunoassay was 10 mug/ml. All the samples measured had detectable levels of FDP with the FgE assay, whereas only 88% were measurable with the TRCHII and 2% by the latex particle agglutination inhibition immunoassay. A comparison of those samples giving a positive result with both the TRCHII and FgE assay showed overall agreement between the results of the two types of assay, but there was considerable scatter of FgE levels at each point of the TRCHII. The major advantages of the radioimmunoassay system are greater sensitivity, specificity and reproducibility.

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Radioimmunoassay of Fibrinogen and Its Proteolysis Products

Cited by 16 publications

References 0 publications

Discussion 10–16

Discussion 10–16

The Development of Radioimmunoassays for Fibrinogen Degradation Products: Fragments D and E

A Comparison between Radioimmunoassay and Other Immunological Techniques for the Measurement of Fibrinogen/Fibrin Degradation Products in Serum

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