Radioimmunoassay of Testosterone During Fetal Development of the Rhesus Monkey1

Resko, John A.; Malley, Arthur; Begley, Donald; Hess, David L.

doi:10.1210/endo-93-1-156

Cited by 148 publications

(32 citation statements)

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“…An equivalent process has been described in the ovary of mice (Pehlemann and Lombard, 1978), rats (AI Mehdi, 1979), guinea-pigs (Guraya, 1977) (Resko et al, 1973 ;Dang and Meusy-Dessolle, 1979) and the guinea-pig (Rigaudière, 1977), for example, agree with microscopic data confirming that the testis passes through a temporary steroidogenesic crisis. The morphological similarities between the fetal testis and ovary suggest that the ovary should also evidence a temporary steroid surge.…”

Section: Introductionsupporting

confidence: 56%

A comparative study of the development of the fetal testis and ovary in the monkey (Macaca fascicularis)

Fouquet¹,

Dang²

1980

Reprod. Nutr. Dévelop.

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Section: Introductionsupporting

confidence: 56%

A comparative study of the development of the fetal testis and ovary in the monkey (Macaca fascicularis)

Fouquet¹,

Dang²

1980

Reprod. Nutr. Dévelop.

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“…Serum testosterone concentrations from individual mice were measured by radioimmunoassay after ether extraction according to published methodology (42). The sensitivity of the assay was 7.8 pg per tube, the inter-assay variation was 8%, and the intra-assay variation was 4.3%.…”

Section: Methodsmentioning

confidence: 99%

Delayed testicular aging in pituitary adenylate cyclase-activating peptide (PACAP) null mice

Lacombe¹,

Lelièvre

Roselli

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

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Age-related decline in male sex hormones is a direct consequence of testicular aging. These changes in the hormonal complement cause physiological disturbances affecting the quality of life for millions of aging men. To assess the influence on testicular aging of pituitary adenylate cyclase-activating peptide (PACAP), a polypeptide that regulates testicular steroidogenesis in vitro, we compared the testicular structure and function between C57BL͞6 wild-type and PACAP ؊/؊ male mice, at 4 and 15 months of age. We show that, in 4-month-old PACAP ؊/؊ mice, steroidogenesis (evaluated by levels of testosterone, steroidogenic acute regulatory protein, 3␤-hydroxysteroid dehydrogenase, and P450c17) was impaired. However, the testicular structure of these animals was not affected. At 15 months of age, wild-type testis displayed typical signs of aging (patchy seminiferous tubules, germ cell depletion, and vacuolization), whereas testicular structure was remarkably well conserved in PACAP ؊/؊ animals. The depletion of germ cells found in wild-type animals was associated with a higher content of peroxynitrites, a marker of reactive oxygen species, and a higher number of apoptotic cells compared with PACAP ؊/؊ mice. Our results show that testicular aging is delayed in PACAP ؊/؊ animals. Because the expression levels of steroidogenic factors are low and constant over time in knockout animals, a proposed mechanism for the protection against testicular degeneration is that production of reactive oxygen species, a byproduct of steroidogenesis that induces apoptosis, is down-regulated in PACAP ؊/؊ animals.Leydig cells ͉ reactive oxygen species ͉ steroidogenesis ͉ neuropeptide ͉ andropause

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“…The serum was stored at K20 8C until further analysis. Serum testosterone concentrations were analyzed by RIA after ether extraction according to previously published methodology (Resko et al 1973). Serum concentrations of luteinizing hormone (LH) and FSH levels were measured by the National Hormone and Peptide Program (Dr A F Parlow, University of California, Los Angeles, CA, USA) using rat LH and FSH RIA kits.…”

Section: Blood Collection and Hormones Measurementsmentioning

confidence: 99%

Lack of vasoactive intestinal peptide reduces testosterone levels and reproductive aging in mouse testis

Lacombe¹,

Lelièvre²,

Roselli³

et al. 2007

Journal of Endocrinology

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The neuropeptides vasoactive intestinal peptide (VIP) and pituitary adenylate cyclase-activating polypeptide have long been considered as putative regulators of testicular functions. In vitro evidence suggests that VIP could play an important role in testosterone biosynthesis. However, the endogenous role of VIP on testicular functions remained to be demonstrated. In C57BL/6 mice exhibiting a complete disruption of the VIP gene, we first observed here that serum testosterone levels were lower than those of WT littermates. At the age of 4 months, this phenotype was accompanied with a reduction of expression of StAR and 3-b-hydroxysteroid dehydrogenase (3b-HSD) in the testis. In addition, serum levels of FSH but not LH were reduced in young knock-out (KO) males. Testicular anatomy also revealed a higher percentage of degenerated seminiferous tubules in the 4-month-old VIP KO animals when compared with WT. In 15-month-old animals, control males showed typical testicular aging, including a severe degeneration of seminiferous tubules, a dramatic decrease in serum levels of testosterone, and a reduction in StAR and 3b-HSD gene expression. In age-matching VIP KO males, the levels of serum testosterone and steroidogenic enzymes were still very low. Interestingly, in contrast to that observed in young mice, testicular degeneration at 15 months was significantly less severe in VIP KO than WT mice. All together, these results suggest that 1) VIP is an important factor for regulating testosterone biosynthesis and FSH secretion and 2) VIP may influence testicular aging.

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Radioimmunoassay of Testosterone During Fetal Development of the Rhesus Monkey1

Cited by 148 publications

References 0 publications

A comparative study of the development of the fetal testis and ovary in the monkey (Macaca fascicularis)

A comparative study of the development of the fetal testis and ovary in the monkey (Macaca fascicularis)

Delayed testicular aging in pituitary adenylate cyclase-activating peptide (PACAP) null mice

Lack of vasoactive intestinal peptide reduces testosterone levels and reproductive aging in mouse testis

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