Radioimmunoassay of Tumour Specific Transplantation Antigen of a Chemically Induced Rat Sarcoma: Circulating Soluble Tumour Antigen in Tumour Bearers

Thomson, David M.; Sellens, V; Eccles, S; Alexander, Peter

doi:10.1038/bjc.1973.163

Cited by 52 publications

(16 citation statements)

References 28 publications

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“…Loss of LA1 reactivity in melanoma after tumor excision was also observed by Marti and T h o m~o n .~~ Vol. 39 Rapid disappearance of measurable antitumor immunity was reported by Unsgaarde and O'Toole and O'Toole, et al with the microcytotoxicity assays in melanoma and bladder cancers. 2841 Moreover, in the leukocyte migration test in breast cancer, Jones, et aLZ0 and Rieche, et al" reported that reactivity rapidly disappeared after the removal of the primary tumor.…”

mentioning

confidence: 84%

An overview: Antitumor immunity in breast cancer assayed by tube leukocyte adherence inhibition

Flores¹,

Marti²,

Grosser³

et al. 1977

Cancer

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The adherence to glass of human peripheral blood leukocytes (PBL) incubated with tumor antigen in vitro, is specifically inhibited if the PBL are sensitized to the antigen. The presence of leukocyte adherence inhibition (LAI) to tumor extracts indicates the presence of systemic antitumor immunity. By the tube leukocyte adherence inhibition assay (tube LAI), it was shown that 85% (191 of 223) Stage I and II, 45% (15 of 34) Stage III and 29% (30 of 103) Stage IV breast cancer patients had LAI reactivity. LAI responsiveness diminished with an increased tumor burden and most patients with advanced cancer exhibited no LAI reactivity. When LAI reactivity was monitored for 1 to 6 months after surgery, 13 of 25 Stage I and II breast cancer patients were negative on the first repeat assay. In general, 7 months after mastectomy most patients clinically free of cancer showed no LAI reactivity. Of thirty-five patients tested between 7 and 18 months after mastectomy, 6 were positive and 4 of the positives had local recurrence. The phenomenon of tube LAI appears to be mediated by monocytes armed with cytophilic antitumor antibody. The serum of patients whose leukocytes responded in the tube LAI assay had free cytophilic antitumor antibody that "armed" or sensitized normal leukocytes to respond in the LAI assay. Serum arming paralleled leukocyte reactivity before and after surgery. Patients with advanced cancer whose leukocytes failed to react in the LAI assay had serum blocking factors (excess tumor antigen) that abrogated the LAI reactivity of leukocytes from reactive patients.

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mentioning

confidence: 84%

An overview: Antitumor immunity in breast cancer assayed by tube leukocyte adherence inhibition

Flores¹,

Marti²,

Grosser³

et al. 1977

Cancer

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“…Macrophages from immune mice can be rendered non-specifically cytotoxic on the addition of antigen, and normal macrophages may become cytotoxic on binding immune complexes on their surface (Evans and Alexander, 1976). A growing sarcoma, causing a continuous presence of circulating antigen or antigen-antibody complexes (Thomson et al, 1973) …”

Section: I)scussionmentioning

confidence: 99%

Non-specific cytotoxicity of spleen cells in mice bearing transplanted chemically induced fibrosarcomas

Mantovani¹,

Evans²,

Alexander³

1977

Br J Cancer

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Spleen cells collected from mice bearing transplanted chemically induced syngeneic fibrosarcomas non-specifically inhibited DNA synthesis of sarcoma and lymphoma target cells in vitro. Splenocytes from mice hyper-immunized against a syngeneic sarcoma specifically inhibited DNA synthesis of the tumour used for immunization. The impairment of tumour-cell DNA synthesis was associated in vitro with cytostasis, and lysis of the target cells was not seen. Since treatment with anti-theta serum and complement did not impair cytostatic action of the spleen cells, and since thymus-deprived animals showed similar activity to normal mice, T lymphocytes were not involved in non-specific cytostasis. Removal of phagocytic adherent cells by carbonyl iron markedly inhibited the cytostatic activity of the spleen cells, suggesting a role in this reaction for cells of the monocyte-macrophage series. The presence of an actively growing sarcoma was a prerequisite for the expression of non-specific cytostasis, since surgical excision resulted in complete disappearance of this activity of spleen cells.

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“…At the present time the major problem limiting progress in this area is the lack of sensitive and quantitative immunoassays for soluble tumour-specific antigens. However, the early work of Thomson et al (1973) using a solid-phase radioimmunoassay for antigen in the serum of rats bearing grafts of a 3-methylcholanthrene-induced sarcoma suggests that it is possible to develop cellfree immunoassays of the required sensitivity, though in more recent studies Thomson et al (1976) have returned to indirect membrane-immunofluoresence tests to monitor the purification of sarcoma-associated antigens.…”

Section: Discussionmentioning

confidence: 99%

Radioiodination of rat hepatoma-specific antigens and retention of serological reactivity

Hannant¹,

Bowen²,

Price³

et al. 1980

Br J Cancer

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Summary.-Papain-solubilized tumour-specific antigens from the aminoazo dyeinduced rat hepatoma D23 were purified by a combination of lectin affinity and immunoadsorbent column chromatography. Isolated antigens were radio-iodinated using three procedures and analysed for their reaction with specific antibodies in syngeneic immune sera by double-antibody co-precipitation tests and by the rebinding of labelled antigens to specific and non-relevant antibodies immobilized on Sepharose-4B. Soluble hepatoma D23-specific antigens were labile to radiolabelling, and for optimal retention of serological reactivity it was necessary to protect the antigenic determinant by performing the chloramine T method of iodination with antigen bound to the immunoadsorbent followed by elution from the solid phase with 3M NaSCN. Immunoadsorption chromatography indicated that one consequence of radiolabelling hepatoma D23-specific antigen with 1'5I was a reduction in the affinity of the labelled antigen for its syngeneic specific antibody.

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Radioimmunoassay of Tumour Specific Transplantation Antigen of a Chemically Induced Rat Sarcoma: Circulating Soluble Tumour Antigen in Tumour Bearers

Cited by 52 publications

References 28 publications

An overview: Antitumor immunity in breast cancer assayed by tube leukocyte adherence inhibition

An overview: Antitumor immunity in breast cancer assayed by tube leukocyte adherence inhibition

Non-specific cytotoxicity of spleen cells in mice bearing transplanted chemically induced fibrosarcomas

Radioiodination of rat hepatoma-specific antigens and retention of serological reactivity

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