Radioimmunodetection of Atherosclerotic Lesions Focusing on the Accumulation Mechanism of Immunoglobulin G

Abstract

In the diagnosis of atherosclerosis, detailed evaluation of biomarkers related to its lesion formation is desired for estimation of its progression rate. In our previous proteomic studies of atherosclerosis mice, the protein level of thrombospondin-4 (TSP4) in the aorta, but not in plasma, elevated relatively with atherosclerotic plaque formation. Therefore, we supposed that TSP4 would be a potential biomarker for diagnostic imaging of atherosclerotic progression. Immunoglobulin G (IgG) has been widely used as a basic molecule of imaging probes providing images specific to their target biomolecules, owing to the antigenantibody reaction. Therefore, we first developed anti-TSP4 monoclonal IgG radiolabeled with 99m Tc ( 99m Tc-TSP4-mAb). 99m Tc-TSP4-mAb showed higher accumulation in atherosclerotic aortas of apoE À/À

Development of a Syrian hamster anti-PD-L1 monoclonal antibody enables oncolytic adenoviral immunotherapy modelling in an immunocompetent virus replication permissive setting

“…Interestingly, we observed a nonsignificant increase in tumour necrosis in the isotype control compared to PBS despite similar tumour growth rate. It is known that mouse IgG2a is capable of Fc-mediated effector function and it has been described that non-specific IgG can accumulate in proinflammatory M1 macrophages via FcgRs and exacerbate inflammation (51). It is possible that the isotype control is inducing an immune reaction which is not sufficient to provide tumour growth control.…”