Radiolabeling efficiency and cell incorporation of chitosan nanoparticles

Gündoğdu, Evren; İlem-Özdemir, Derya; Ekinci, Meliha; Özgenç, Emre; Aşıkoğlu, Makbule

doi:10.1016/j.jddst.2015.06.018

Cited by 21 publications

(12 citation statements)

References 28 publications

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“…12,13 CSH, the deacetylated derivative of chitin, is mostly preferred as the coating polymer, because it is a cationic, nontoxic, biocompatible, and biodegradable polymer and has mucoadhesive, antibacterial, antifungal, antitumor, and stimulating immunoenhancing properties. [14][15][16][17] The enhanced mucoadhesive property following CSH coating is attributed to increased retention of the formulation at the buccal surface. 18 CSH-coated NPs have reached an important position in the arena of drug delivery, and thus, it appears quite relevant to describe and compare various methods explored for preparation and characterization of CSH-coated NPs.…”

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confidence: 99%

Development, characterization, and in vivo assessment of mucoadhesive nanoparticles containing fluconazole for the local treatment of oral candidiasis

Rençber¹,

Karavana²,

Yılmaz³

et al. 2016

IJN

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This study aimed to develop a suitable buccal mucoadhesive nanoparticle (NP) formulation containing fluconazole for the local treatment of oral candidiasis. The suitability of the prepared formulations was assessed by means of particle size (PS), polydispersity index, and zeta potential measurements, morphology analysis, mucoadhesion studies, drug entrapment efficiency (EE), in vitro drug release, and stability studies. Based on the optimum NP formulation, ex vivo drug diffusion and in vitro cytotoxicity studies were performed. Besides, evaluation of the antifungal effect of the optimum formulation was evaluated using agar diffusion method, fungicidal activity-related in vitro release study, and time-dependent fungicidal activity. The effect of the optimum NP formulation on the healing of oral candidiasis was investigated in an animal model, which was employed for the first time in this study. The zeta potential, mucoadhesion, and in vitro drug release studies of various NP formulations revealed that chitosan-coated NP formulation containing EUDRAGIT ® RS 2.5% had superior properties than other formulations. Concerning the stability study of the selected formulation, the formulation was found to be stable for 6 months. During the ex vivo drug diffusion study, no drug was found in receptor phase, and this is an indication of local effect. The in vitro antifungal activity studies showed the in vitro efficacy of the NP against Candida albicans for an extended period. Also, the formulation had no cytotoxic effect at the tested concentration. For the in vivo experiments, infected rabbits were successfully treated with local administration of the optimum NP formulation once a day. This study has shown that the mucoadhesive NP formulation containing fluconazole is a promising candidate with once-a-day application for the local treatment of oral candidiasis.

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confidence: 99%

Development, characterization, and in vivo assessment of mucoadhesive nanoparticles containing fluconazole for the local treatment of oral candidiasis

Rençber¹,

Karavana²,

Yılmaz³

et al. 2016

IJN

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“…A significant increase in cell death was also detected in all cell lines treated with cisplatin or GHCl [32]. Gundogdu et al published an article about the cancer cell targeting potential of newly developed cationic nanoparticles of chitosan by radioactive studies with cell culture studies [33]. The results revealed that, the chitosan nanoparticles show higher uptake in bone cancer cell lines than normal bone cells.…”

Section: Cell Culture Studiesmentioning

confidence: 97%

Radiolabeling and cell incorporation studies of gemcitabine HCl microspheres on bladder cancer and papilloma cell line

İlem-Özdemir

Karavana

Şenyiğit

et al. 2016

J Radioanal Nucl Chem

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In the present study incorporation differences between the developed formulations for treatment for bladder cancer was investigated by radioactive cell culture studies. Developed bioadhesive microspheres (MS) and gemcitabinehydrochloride loaded MS (GHCl-MS) were radiolabeled. After observing the optimum labeling conditions, 99m Tc-MS and 99m Tc-GHCl-MS were loaded to the chitosan gel (CG) and poloxamer gel. The in vitro cell incorporation affinity of newly developed formulations to bladder papilloma (RT4) and the carcinoma (T24) cell lines was investigated. Cell culture studies results indicate that the use of prolonged release bioadhesive CG formulation was highly improved the targeting of the GHCl to the cancer cells. Graphical Abstract

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“…Technetium‐99m labeling usually pertains tagging the radionuclide on the organic material, by direct labeling, via their available donor atoms such as the free amine groups of chitosan, octadecylamine, polyethyleneimine, the carboxylates of poly‐ γ ‐glutamic acid, and poly( d , l ‐lactic‐co‐glycolic acid), as well as the thiol groups of bovine serum albumin. In other examples, the organic material is functionalized with a chelator, such as DTPA . A different labeling method of an iron oxide nanoparticle where a 99m Tc‐bisphosphonate complex was introduced on the iron oxide surface was reported recently .…”

Section: Technetium‐99m Labeling Strategies For the Development Of Tamentioning

confidence: 99%

“…In other examples, the organic material is functionalized with a chelator, such as DTPA. [154][155][156][157][158][159][160][161][162] A different labeling method of an iron oxide nanoparticle where a 99m Tc-bisphosphonate complex was introduced on the iron oxide surface was reported recently. 163 In another method, the coating material of the magnetic nanoparticle was conjugated to guanine via glutaraldehyde and was labeled with [ 99m Tc(CO) 3 ] + .…”

Section: Technetium-99m Labeling Of Nanoparticlesmentioning

confidence: 99%

Technetium‐99m radiochemistry for pharmaceutical applications

Papagiannopoulou

2017

Labelled Comp Radiopharmac

125

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Technetium-99m ( Tc) is a widely used radionuclide, and the development of Tc imaging agents continues to be in demand. This overview discusses basic principles of Tc radiopharmaceutical preparation and design and focuses on the Tc radiochemistry relevant to its pharmaceutical applications. The Tc complexes are described based on the most typical examples in each category, keeping up with the state-of-the-art in the field. In addition, the main current strategies to develop targeted Tc radiopharmaceuticals are summarized.

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Radiolabeling efficiency and cell incorporation of chitosan nanoparticles

Cited by 21 publications

References 28 publications

Development, characterization, and in vivo assessment of mucoadhesive nanoparticles containing fluconazole for the local treatment of oral candidiasis

Development, characterization, and in vivo assessment of mucoadhesive nanoparticles containing fluconazole for the local treatment of oral candidiasis

Radiolabeling and cell incorporation studies of gemcitabine HCl microspheres on bladder cancer and papilloma cell line

Technetium‐99m radiochemistry for pharmaceutical applications

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