Radioprotection of Hematopoietic Tissues in Mice by Lipoic Acid

Ramakrishnan, N.; Wolfe, William W.; Catravas, George N.

doi:10.2307/3578382

Cited by 61 publications

(24 citation statements)

References 25 publications

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“…If correct, this implies that undefined oxidative events are necessary if a cell is to successfully undergo apoptosis. The ability of thiol-containing antioxidants such as N-acetyl cysteine (NAC) or N-(2-mercaptoethyl)-1,3-propanediamine to similarly confer protection against apoptosis supports this hypothesis [10][11][12]. In addition, Flomerfelt et al report elevated expression of a glutathione Stransferase gene at an early stage in dexamethasone-induced lymphocyte apoptosis [13].…”

Section: Introductionmentioning

confidence: 84%

Nitrone spin traps and a nitroxide antioxidant inhibit a common pathway of thymocyte apoptosis

Slater

Nobel

Maellaro

et al. 1995

Biochemical Journal

181

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Oxidative stress has recently been suggested to be a mediator of apoptotic cell death [Buttke and Sandstrom (1994) Immunology Today 15, 7-10], although evidence that this phenomenon is a widespread component of apoptosis is lacking. When rat thymocytes were exposed to the glucocorticoid methylprednisolone (MPS), a progressive increase in intracellular peroxides and a decrease in glutathione (GSH) were observed to accompany the onset of apoptosis. Using Percoll density gradients to isolate subpopulations of thymocytes at different stages of apoptosis, the increase in peroxide content was found to be restricted to apoptotic cells, while a significant depletion of GSH and reduced protein thiol was detected in both pre-apoptotic and fully apoptotic cells. To investigate the biological significance of these redox changes, the free radical spin traps 5,5-dimethyl-1-pyrroline-1-oxide (DMPO) and 3,3,5,5-tetramethyl-1-pyrroline-1-oxide (TMPO), and the related nitroxide-radical antioxidant 2,2,6,6-tetramethyl-1-piperidinyl-1-oxyl (TEMPO) were tested as inhibitors of thymocyte apoptosis. The cell shrinkage and DNA fragmentation induced by four different initiators of apoptosis were reduced by each compound. TEMPO inhibition of both etoposide- and MPS-induced thymocyte DNA fragmentation was also found to correlate with an increase in intracellular GSH, providing support for the proposal that its antioxidant properties were responsible for the observed protective activity. We conclude that some form of intracellular oxidation (here measured indirectly by changes in intracellular GSH and peroxide levels) is required during thymocyte apoptosis even when this process is initiated by an agent that does not exert a direct oxidant action.

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Section: Introductionmentioning

confidence: 84%

Nitrone spin traps and a nitroxide antioxidant inhibit a common pathway of thymocyte apoptosis

Slater

Nobel

Maellaro

et al. 1995

Biochemical Journal

181

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“…Like a-LA, DHLA is also an antioxidant, probably more potent than its oxidized counterpart. The DHLA/a-LA couple has a redox potential (DE) of -320 mV, which means that DHLA has one of the highest antioxidant potentials known in biological systems [8]. The ratio of DHLA to a-LA is also a good measure of the oxidative stress to which an organism is exposed [4].…”

mentioning

confidence: 99%

Simultaneous Determination of the Endogenous Free α-Lipoic Acid and Dihydrolipoic Acid in Human Plasma and Erythrocytes by RP-HPLC with Electrochemical Detection

et al. 2011

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A highly sensitive, precise, and accurate reversed-phase high-performance liquid-chromatography/ electrochemical detection method for simultaneous determination of the endogenous free a-lipoic acid and dihydrolipoic acid in biological matrices was developed and validated. The two analytes were extracted from the samples with acetonitrile/10% metaphosphoric acid solution (aqueous) (50/50 v/v). To determine the total lipoic acid, samples were treated with tris(2-carboxyethyl)phosphine solution in phosphate buffer, pH 2.5 with 85% orthophosphoric acid prior to deproteination. The two analytes were separated on a C 18 (150 9 4.6 mm, 5 lm) analytical column using acetonitrile-50 mM phosphate buffer, pH 2.5 with 85% orthophosphoric acid (35/65 v/v) as the isocratic mobile phase pumped at a flow rate of 2.0 mL min -1 at the column oven temperature of 35°C. The column eluents were monitored at a potential of 0.9 V. These analytes were efficiently resolved in \7 min. The present method was sufficiently robust and specific for simultaneous determination of the two analytes and demonstrated acceptable values for linearity (r 2 = 0.999 in the range of 0.1-500 and 0.25-1,000 ng mL -1 for a-lipoic acid and dihydrolipoic acid, respectively), recovery ([97%), precision (RSD% \2), and sensitivity (on column limit of detection, 150 and 375 fg for a-lipoic acid and dihydrolipoic acid, respectively and limit of quantification: 0.5 and 1.25 pg for a-lipoic acid and dihydrolipoic acid, respectively), indicating that the proposed method was more sensitive, precise, economical, and versatile, and has higher throughput than the previously reported methods for simultaneous determination of the two analytes.

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“…Several synthetic compounds such as 2-mercaptopropionylglycine (Sugahara et al, 1970;Saini et al, 1978;Ayene et al, 1988), WR-2721 (Yuhas et al, 1980), lipoic acid (Ramakrishnan et al, 1992), deoxyspergualin (Nemoto et al, 1995), and dipyridamole and adenine monophosphate (Pospisil et al, 1995) have been tested for their protective action against radiation. Owing to their inherent toxicity, these have not been successful in the field of clinical radiotherapy.…”

Section: Introductionmentioning

confidence: 99%

Modulation of serum phosphatases activity in Swiss albino mice against gamma irradiation by Mentha piperita Linn.

Samarth

Goyal

Kumar

2002

Phytotherapy Research

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The modulatory influence of mentha oil (Mentha piperita Linn.) against a lethal dose (8.0 Gy) of gamma irradiation on the activities of serum phosphatases in Swiss albino mice was studied at various post-irradiation intervals between 6 h and 30 days. Mentha oil (40 microL/animal/day) given orally for 3 consecutive days prior to whole-body irradiation (8.0 Gy) showed a modulation of activity of serum phosphatases. The values of acid phosphatase activities were significantly higher in the irradiated groups throughout the experiment compared with the mentha treated unirradiated animals. However, the acid phosphatase activity of mentha treated irradiated animals showed a significant decline over untreated irradiated animals at all autopsy intervals, which attained the normal value on day 5. On the contrary, a marked decrease in serum alkaline phosphatase activity was noted in both irradiated groups but in the mentha treated irradiated group the values of alkaline phosphatase activity were found to be significantly higher than the respective control during the period of study being normal at day 5 post-irradiation and onwards.

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Radioprotection of Hematopoietic Tissues in Mice by Lipoic Acid

Cited by 61 publications

References 25 publications

Nitrone spin traps and a nitroxide antioxidant inhibit a common pathway of thymocyte apoptosis

Nitrone spin traps and a nitroxide antioxidant inhibit a common pathway of thymocyte apoptosis

Simultaneous Determination of the Endogenous Free α-Lipoic Acid and Dihydrolipoic Acid in Human Plasma and Erythrocytes by RP-HPLC with Electrochemical Detection

Modulation of serum phosphatases activity in Swiss albino mice against gamma irradiation by Mentha piperita Linn.

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