Radioprotective Effect of <i>Ocimum sanctum</i> and Amifostine on the Salivary Gland of Rats After Therapeutic Radioiodine Exposure

Joseph, Lebana J.; Bhartiya, Uma S.; Raut, Y; Hawaldar, Rohini; Nayak, Yogendra; Pawar, Yogita; Jambhekar, Nirmala A.; Rajan, M. G. R.

doi:10.1089/cbr.2011.1014

Cited by 21 publications

(14 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, this study has some limitations that warrant consideration. First, an RAI dose of 0.01 mCi/g body weight was administered based on a review of related animal studies (0.004-0.0216 mCi/g body weight) (20,(24)(25)(26). According to our preliminary study, this dose appeared to be sufficient to damage SGs without compromising general health.…”

Section: Discussionmentioning

confidence: 99%

Morphometric and Functional Changes of Salivary Gland Dysfunction After Radioactive Iodine Ablation in a Murine Model

Choi

Park

Kim

et al. 2013

Thyroid

View full text Add to dashboard Cite

Background: Ablation of the thyroid tissue using radioactive iodine (RAI) after the surgical removal of welldifferentiated thyroid cancer can induce radiation-related salivary gland (SG) dysfunction. However, in vivo changes of SGs after RAI administration in appropriate animal models are not well described in the literature. This study was undertaken to document morphometric and functional changes during the 12 months after RAI administration in a murine model of RAI-induced SG dysfunction. Methods: Four-week-old female C57BL/6 mice (n = 60) were divided into an RAI-treated group (n = 30) that received RAI orally (0.01 mCi/g body weight) and an unexposed control group (n = 30). Mice in both groups were divided into five subgroups (n = 6 per subgroup) and euthanized at 1, 2, 3, 6, and 12 months post-RAI administration. Salivary flow rates and salivary lag times were measured at 1, 2, 3, 6, and 12 months after RAI administration. Morphological and histological examinations and terminal deoxynucleotidyl transferase dUTP nick end labeling assays were performed. In addition, changes in salivary 99mTc pertechnetate uptake and excretion were observed by single-photon emission computed tomography. Results: In RAI-treated mice, the SGs were significantly lighter than those of unexposed controls at all study time points. Lag times to salivation in the RAI-treated group were greater than in the unexposed controls, but mean salivary flow rates were lower. Histologic examinations of SGs in the RAI group showed pale cytoplasm, atypical ductal configuration, septal widening, cytoplasmic vacuolization with pleomorphism, lymphocyte infiltration, and increased fibrosis. Furthermore, more apoptotic cells were observed in acini and ducts in the RAI group. In addition, patterns of 99m Tc pertechnetate uptake and excretion in the RAI group were quite different from those observed in controls at 1 and 12 months post-RAI. Conclusion: Various histological alterations were observed in mice exposed to RAI, that is, an increase in apoptotic acini and ductal cells and functional SG deterioration. The murine model of RAI-induced SG dysfunction used in the present study appears to be applicable to preclinical research on RAI-induced sialadenitis in patients with well-differentiated thyroid cancer.

show abstract

Section: Discussionmentioning

confidence: 99%

Morphometric and Functional Changes of Salivary Gland Dysfunction After Radioactive Iodine Ablation in a Murine Model

Choi

Park

Kim

et al. 2013

Thyroid

View full text Add to dashboard Cite

show abstract

“…RI-exposed SGs showed cellular injury, acinar loss and disorganization, glandular duct enlargement, and marked lipomatosis [4,27]. In a previous study, SGs in RI-exposed mice displayed pale cytoplasm, atypical ductal configuration, septal widening, lymphocyte infiltration, cytoplasmic vacuolization with pleomorphism, and elevated levels of tissue fibrosis [28].…”

Section: Discussionmentioning

confidence: 99%

“…Joseph et al [4] also showed that antioxidant ( Ocimum sanctum ) or amifostine pretreated SGs in rats exhibited greater 99m Tc pertechnetate excretion than an RI alone. In the present study, we investigated spatial distributions of 99m Tc pertechnetate uptake and its excretion from SGs.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Radioprotective Effect of Epigallocatechin-3-Gallate on Salivary Gland Dysfunction After Radioiodine Ablation in a Murine Model

Choi

Park

et al. 2016

Clin Exp Otorhinolaryngol

View full text Add to dashboard Cite

Objectives.Radioiodine (RI) therapy is known to subject cellular components of salivary glands (SG) to oxidative stress leading to SG dysfunction. However, the protective effects of antioxidants on RI-induced SG damage have not been well investigated. The authors investigated the morphometric and functional effects of epigallocatechin-3-gallate (EGCG) administered prior to RI therapy and compared this with the effects of amifostine (a well-known antioxidant) in a murine model of RI sialadenitis.Methods.Four-week-old female C57BL/6 mice (n=48) were divided into four groups; a normal control group, a RI-treated group (0.01 mCi/g mouse, orally), an EGCG and RI-treated group, and an amifostine and RI-treated group. Animals in these groups were divided into 3 subgroups and euthanized at 15, 30, and 90 days post-RI treatment. Salivary flow rates and lag times were measured, and morphologic and histologic examinations and TUNEL (terminal deoxynucleotidyl transferase biotin-dUDP nick end labeling) assays were performed. Changes in salivary 99mTc pertechnetate uptake and excretion were followed by single-photon emission computed tomography.Results.Salivary flow rates and lag times to salivation in the EGCG or amifostine groups were better than in the RI-treated group. Histologic examinations of SGs in the EGCG or amifostine group showed more mucin-rich parenchyma and less periductal fibrosis than in the RI-treated group. Fewer apoptotic cells were observed in acini, ducts, and among endothelial cells in the EGCG or amifostine group than in the RI group. In addition, patterns of 99mTc pertechnetate excretion were quite different in the EGCG or amifostine group than in the RI group.Conclusion.EGCG supplementation before RI therapy could protect from RI-induced SG damage in a manner comparable to amifostine, and thus, offers a possible means of preventing SG damage by RI.

show abstract

“…Thus results suggests potential radioprotective effect of O. sanctum and amifostine against high-dose (131) I exposure. [33] Two flavonoids, orientin and vicenin, isolated from the leaves of the Indian plant O. sanctum were investigated their radioprotective role in mice. Both compounds resulted in protection against death from gastrointestinal syndrome as well as bone marrow syndrome when injected intraperitoneally (i.p.)…”

Section: Withania Somnifera (Ashwagandha)mentioning

confidence: 99%

A review of radioprotective plants

Bhandari¹

2013

Int J Green Pharm

View full text Add to dashboard Cite

Radioprotective compounds have been used to diminish morbidity or mortality produced by ionizing irradiation. Initial developments of such agents concentrated on thiol synthetic compounds, like amifostine. This agent decreased mortality; however, there were difficulties in administering aminothiols that led to adverse effects. Unfortunately, no ideal, safe synthetic radioprotectors are available to date; hence, the exploration for other sources, including plants, has been ongoing for several decades. A methodical screening strategy can offer leads to isolating prospective novel candidate drugs from plant sources, for alleviation of radiation injury. This article reviews some of the most promising plants, and their bioactive principles, that are extensively used in traditional systems of medicine, and which have rendered noteworthy radioprotection in both in vitro and in vivo model systems.

show abstract

Radioprotective Effect of Ocimum sanctum and Amifostine on the Salivary Gland of Rats After Therapeutic Radioiodine Exposure

Cited by 21 publications

References 24 publications

Morphometric and Functional Changes of Salivary Gland Dysfunction After Radioactive Iodine Ablation in a Murine Model

Morphometric and Functional Changes of Salivary Gland Dysfunction After Radioactive Iodine Ablation in a Murine Model

Radioprotective Effect of Epigallocatechin-3-Gallate on Salivary Gland Dysfunction After Radioiodine Ablation in a Murine Model

A review of radioprotective plants

Contact Info

Product

Resources

About