Radiosensitizing effect of diosmetin on radioresistant lung cancer cells via Akt signaling pathway

Xu, Zhijie; Yan, Yuanliang; Xiao, Lingfang; Dai, Shuang; Zeng, Shuangshuang; Qian, Long; Wei, Lin; Yang, Xue; Xiao, Yi; Gong, Zhicheng

doi:10.1371/journal.pone.0175977

Cited by 41 publications

(34 citation statements)

References 35 publications

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“…Clonogenic studies were used to determine the cells’ ability to form colonies after different treatment conditions [ 26 ]. Data was adjusted according to initial number of cells seeded per treatment condition.…”

Section: Resultsmentioning

confidence: 99%

Exposure of Breast and Lung Cancer Cells to a Novel Estrone Analog Prior to Radiation Enhances Bcl-2-Mediated Cell Death

Nolte

Joubert

Lakier

et al. 2018

IJMS

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Following exposure of cells to gamma-radiation, a cascade of intracellular consequences may be observed in a semitemporal manner. This includes deoxyribonucleic acid (DNA) damage and reactive oxygen species (ROS) accumulation initially, with consequent signaling for DNA repair and facilitative regulation of the cell cycle. Failure to rectify the damage or ROS levels leads to induction of senescence or apoptosis. 2-Ethyl-3-O-sulfamoyl-estra-1,3,5(10),15-tetraen-17-ol (ESE-15-ol), a 2-methoxyestradiole analog designed in silico for superior pharmacokinetics, was investigated for its potential to enhance apoptotic signaling and decrease the long-term survival of cells exposed to radiation. Sequential early intracellular effects within radiation-treated MCF-7 breast- and A549 lung cancer cells pre-exposed to low-dose ESE-15-ol were investigated using various flow cytometric protocols, spectrophotometry, and microscopy. Long-term cellular survival and proliferation was examined using clonogenic studies, which demonstrated a significant decrease in the presensitized cells. Combination-treated cells exhibited increased superoxide formation, and decreased Bcl-2 expression and -phosphorylation. Induction of apoptosis and elevation of the sub-G1 phase was evident in the pre-exposed MCF-7 cells, although only minimally in the A549 cells at 48-h. These results indicate that low-dose ESE-15-ol may increase tumor response to radiation. Future studies will investigate the effect of ESE-15-ol pre-exposure on radiation-induced DNA damage and repair mechanisms.

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Section: Resultsmentioning

confidence: 99%

Exposure of Breast and Lung Cancer Cells to a Novel Estrone Analog Prior to Radiation Enhances Bcl-2-Mediated Cell Death

Nolte

Joubert

Lakier

et al. 2018

IJMS

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“…These studies have highlighted that diosmetin is converted into luteolin and another product not characterized by cytochrome P450 CYP1 enzymes [131][132][133]. In all in vitro studies, diosmetin administration led to an increase of ROS production, to the arrest of the cells in G0/G1, G1/S, or G2/M phase, dependent on the typology of cancer cell model used, and induction of apoptosis pathway in a dose-dependent manner [134][135][136][137]. The mechanisms influenced by diosmetin are different and, probably, cell specific.…”

Section: Diosmetinmentioning

confidence: 99%

Citrus Flavones: An Update on Sources, Biological Functions, and Health Promoting Properties

Barreca

Mandalari

Calderaro

et al. 2020

Plants

103

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Citrus spp. are among the most widespread plants cultivated worldwide and every year millions of tons of fruit, juices, or processed compounds are produced and consumed, representing one of the main sources of nutrients in human diet. Among these, the flavonoids play a key role in providing a wide range of health beneficial effects. Apigenin, diosmetin, luteolin, acacetin, chrysoeriol, and their respective glycosides, that occur in concentrations up to 60 mg/L, are the most common flavones found in Citrus fruits and juices. The unique characteristics of their basic skeleton and the nature and position of the substituents have attracted and stimulated vigorous investigations as a consequence of an enormous biological potential, that manifests itself as (among other properties) antioxidant, anti-inflammatory, antiviral, antimicrobial, and anticancer activities. This review analyzes the biochemical, pharmacological, and biological properties of Citrus flavones, emphasizing their occurrence in Citrus spp. fruits and juices, on their bioavailability, and their ability to modulate signal cascades and key metabolic enzymes both in vitro and in vivo. Electronic databases including PubMed, Scopus, Web of Science, and SciFinder were used to investigate recent published articles on Citrus spp. in terms of components and bioactivity potentials.

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“…In recent years, there has been increased interest in the natural products from plants that play important roles in research and development of therapeutics agents for cancer. For example, many antitumor drugs in clinical used, such as podophyllotoxins ( 5 ), diosmetins ( 6 ), and taxanes ( 7 ) are taken from plants.…”

Section: Introductionmentioning

confidence: 99%

The Antitumor Activities of Marsdenia tenacissima

et al. 2018

Self Cite

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Marsdenia tenacissima (MT), a traditional Chinese herbal medicine, has long been used for thousands of years to treat asthma, tracheitis, rheumatism, etc. An increasing number of recent studies have focused on the antitumor effects of MT. The effects of MT on cancer are the result of various activated signaling pathways and inhibiting factors and the high expression levels of regulatory proteins. MT can inhibit different cancer types including non-small cell lung cancer (NSCLC), malignant tumors, hepatic carcinoma, and so on. This article mainly focuses on the activities and mechanisms of MT. In addition, the efficacy and toxicity of MT are also discussed. Further studies of MT are required for improved medicinal utilization.

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Radiosensitizing effect of diosmetin on radioresistant lung cancer cells via Akt signaling pathway

Cited by 41 publications

References 35 publications

Exposure of Breast and Lung Cancer Cells to a Novel Estrone Analog Prior to Radiation Enhances Bcl-2-Mediated Cell Death

Exposure of Breast and Lung Cancer Cells to a Novel Estrone Analog Prior to Radiation Enhances Bcl-2-Mediated Cell Death

Citrus Flavones: An Update on Sources, Biological Functions, and Health Promoting Properties

The Antitumor Activities of Marsdenia tenacissima

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