Radish

Kaneko, Yukio; Kimizuka-Takagi, Chiaki; Bang, Sang Woo; Matsuzawa, Yasuo

doi:10.1007/978-3-540-34536-7_3

Cited by 25 publications

(25 citation statements)

References 103 publications

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“…longipinnatus Bailey). According to most opinions, R. sativus L. was originated from wild radish R. raphanistrum L. or was derived by hybridization between R. maritimus and R. landra [ 16 , 17 ]. In recent years, the RadishBase [ 18 ], a genomic database of radish, was developed [ 19 ], and comprehensive analysis of expressed sequence tags from cultivated and wild radish was performed [ 20 ].…”

Section: Introductionmentioning

confidence: 99%

“…Some researchers [ 26 , 27 ] noted that zone of secondary xylem in radish storage root (mainly an inner region of secondary xylem) also includes numerous little foci of cambium-like secondary meristem (so called “meristematic foci”) which are maintained for a limited time and give rise to a small number of tertiary conductive elements. On the other hand, R. raphanistrum , presumable ancestor of R. sativus [ 16 , 17 ] demonstrate less extensive secondary thickening and does not form storage root. The main differences in the anatomical structure of R. sativus and R. raphanistrum are less extensive zone of secondary xylem and significantly fewer cells of the storage parenchyma in the root of R. raphanistrum (Fig.…”

Section: Introductionmentioning

confidence: 99%

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Identification, expression, and functional analysis of CLE genes in radish (Raphanus sativus L.) storage root

et al. 2016

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BackgroundRadish (Raphanus sativus L.) is a widespread agricultural plant forming storage root due to extensive secondary growth which involves cambium proliferation and differentiation of secondary conductive tissues. Closely related to the model object Arabidopsis thaliana, radish is a suitable model for studying processes of secondary growth and storage root development. CLE peptides are a group of peptide phytohormones which play important role in the regulation of primary meristems such as SAM, RAM, and procambium, as well as secondary meristems. However, the role of CLE peptides in lateral growth of root during storage root formation has not been studied to date.ResultsIn present work we studied the role of CLE peptides in the development of storage root in radish. We have identified 18 CLE genes of radish (RsCLEs) and measured their expression in various plant organs and also at different stages of root development in R. sativus and Raphanus raphanistrum—its close relative which does not form storage root. We observed significant decline of expression levels for genes RsCLE1, 2, 11, 13, and 16, and also multifold increase of expression levels for genes RsCLE19, and 41 during secondary root growth in R. sativus but not in R. raphanistrum. Expression of RsCLE 2, 19, and 41 in R. sativus root was confined to certain types of tissues while RsCLE1, 11, 13, and 16 expressed throughout the root. Experiments on overexpression of RsCLE2, 19 and 41 or treatment of radish plants with synthetic CLE peptides revealed that CLE19 and CLE2 increase the number of xylem elements, and CLE41 induces the formation of extra cambium foci in secondary xylem. Expression levels of RsCLE2 and 19 strongly decrease in response to exogenous cytokinin, while auxin causes dramatic increase of RsCLE19 expression level and decrease of RsCLE41 expression.ConclusionsOur data allow us to hypothesize about the role of RsCLE2, 19 and 41 genes in the development of storage root of Raphanus sativus, e.g. RsCLE19 may play a role in auxin-dependent processes of xylem differentiation and RsCLE41 stimulates cambium activity.Electronic supplementary materialThe online version of this article (doi:10.1186/s12870-015-0687-y) contains supplementary material, which is available to authorized users.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Identification, expression, and functional analysis of CLE genes in radish (Raphanus sativus L.) storage root

et al. 2016

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“…landra . Most reports suggest that R. sativus L. originated from R. raphanistrum L., but it has also been proposed that R. sativus resulted from hybridization between R. maritimus and R. landra , or alternatively that it derived from interspecific hybridization between a variety of parental species [ 18 - 20 ].…”

Section: Introductionmentioning

confidence: 99%

Comprehensive analysis of expressed sequence tags from cultivated and wild radish (Raphanus spp.)

et al. 2013

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BackgroundRadish (Raphanus sativus L., 2n = 2× = 18) is an economically important vegetable crop worldwide. A large collection of radish expressed sequence tags (ESTs) has been generated but remains largely uncharacterized.ResultsIn this study, approximately 315,000 ESTs derived from 22 Raphanus cDNA libraries from 18 different genotypes were analyzed, for the purpose of gene and marker discovery and to evaluate large-scale genome duplication and phylogenetic relationships among Raphanus spp. The ESTs were assembled into 85,083 unigenes, of which 90%, 65%, 89% and 89% had homologous sequences in the GenBank nr, SwissProt, TrEMBL and Arabidopsis protein databases, respectively. A total of 66,194 (78%) could be assigned at least one gene ontology (GO) term. Comparative analysis identified 5,595 gene families unique to radish that were significantly enriched with genes related to small molecule metabolism, as well as 12,899 specific to the Brassicaceae that were enriched with genes related to seed oil body biogenesis and responses to phytohormones. The analysis further indicated that the divergence of radish and Brassica rapa occurred approximately 8.9-14.9 million years ago (MYA), following a whole-genome duplication event (12.8-21.4 MYA) in their common ancestor. An additional whole-genome duplication event in radish occurred at 5.1-8.4 MYA, after its divergence from B. rapa. A total of 13,570 simple sequence repeats (SSRs) and 28,758 high-quality single nucleotide polymorphisms (SNPs) were also identified. Using a subset of SNPs, the phylogenetic relationships of eight different accessions of Raphanus was inferred.ConclusionComprehensive analysis of radish ESTs provided new insights into radish genome evolution and the phylogenetic relationships of different radish accessions. Moreover, the radish EST sequences and the associated SSR and SNP markers described in this study represent a valuable resource for radish functional genomics studies and breeding.

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“…Radish, Raphanus sativus L. (2n = 2x = 18), is one of the most important vegetables in East Asia, along with other cruciferous crop plants (Kitamura 1958, Kaneko et al 2007. Its hypertrophied root with hypocotyl is mainly used in cooking, as a pickled vegetable, and in salads.…”

Section: Introductionmentioning

confidence: 99%

Mapping of QTLs controlling root shape and red pigmentation in radish, Raphanus sativus L.

et al. 2008

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Genetic factors controlling root shape and red pigmentation on the surface of the hypocotyl (upper part of root) were investigated using a molecular linkage map based on an F 2 population derived from a cross between two radish cultivars, 'Huang-he hong-wan' and 'Utsugi-gensuke'. One hundred and ninety-eight segregating markers (169 AFLPs, 28 Brassica SSRs and one SLG-CAPS) were located on 14 linkage groups (LGs) at a LOD threshold value of 5.0. The mapping position of Brassica SSRs revealed that frequent genomic rearrangements occurred between the Brassica and Raphanus genomes. Three quantitative trait loci (QTLs) for root shape with LOD values of 2.42, 3.22 and 2.88 were identified on LGs 3, 8 and 9, respectively. These three QTLs accounted for 42.4% of the phenotypic variance when joined together. Analysis of QTL(s) for root diameter revealed the presence of two QTLs on LG4 and LG8. The QTL on LG8 was considered to control root thickening and to affect the root shape. For hypocotyl pigmentation, a QTL which exerted a large genetic effect with a LOD value of 9.58, was recognized on LG 11 and accounted for 43.8% of the phenotypic variance.

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Radish

Cited by 25 publications

References 103 publications

Identification, expression, and functional analysis of CLE genes in radish (Raphanus sativus L.) storage root

Identification, expression, and functional analysis of CLE genes in radish (Raphanus sativus L.) storage root

Comprehensive analysis of expressed sequence tags from cultivated and wild radish (Raphanus spp.)

Mapping of QTLs controlling root shape and red pigmentation in radish, Raphanus sativus L.

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