Radloimmunological Measurement of Follicle Stimulating Hormone and Prolactin in the Dog

Reimers, T. J.; Phemister, Robert D.; Niswender, G. D.

doi:10.1095/biolreprod19.3.673

Cited by 43 publications

(28 citation statements)

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“…The daily dosage of bromocriptine used in the present study (OT mg/kg/dog) was similar to the dose ( 1 mg/dog) previously demonstrated to reduce serum prolactin concentrations in dogs to 25% of preinjection levels after a single injection (Reimers et al, 1978). The effects of bromo¬ criptine could have involved changes in secretion of hormones other than prolactin, including decreased LH secretion (Lachelin et al, 1977;Shaban & Terranova, 1986 (Okkens et al, 1985).…”

Section: Discussionsupporting

confidence: 66%

Suppression of luteal function in dogs by luteinizing hormone antiserum and by bromocriptine

Concannon

Weinstein²,

Whaley³

et al. 1987

Reproduction

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Section: Discussionsupporting

confidence: 66%

Suppression of luteal function in dogs by luteinizing hormone antiserum and by bromocriptine

Concannon

Weinstein²,

Whaley³

et al. 1987

Reproduction

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“…The measurement of plasma LH and FSH concentrations before and after the administration of a GnRH analogue permits assessment of the capacity of the pituitary to secrete gonadotropins under different reproductive conditions. In agreement with the findings of others, GnRH administration elicited a distinct release of LH [4,[7][8][9][10] and FSH [10]. The course of the GnRH-induced surges in FSH and LH in the anestrous bitches was comparable to what has been reported previously for spontaneous FSH and LH pulses in anestrous bitches [1].…”

Section: Discussionsupporting

confidence: 91%

“…However, the used GnRH dosage in the latter study was minimal. Only one study was concerned with the concomitant secretion of the gonadotropins in the bitch after GnRH administration and showed that in cyclic bitches FSH and LH levels increased within 15 min after GnRH stimulation [10].…”

Section: Introductionmentioning

confidence: 99%

Basal and GnRH-induced secretion of FSH and LH in anestrous versus ovariectomized bitches

et al. 2007

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The basal and gonadotropin releasing hormone (GnRH)-induced plasma concentrations of follicle stimulating hormone (FSH) and luteinizing hormone (LH) were studied in four anestrous and four ovariectomized (OVX) bitches. Blood samples were obtained via jugular venipuncture 40 min before and 0, 10, 20, 30, 60, 90, and 120 min after the i.v. administration of synthetic GnRH in a dose of 10 mg/kg body weight.The basal plasma FSH and LH concentrations were significantly higher in the OVX bitches than in the anestrous bitches. In the anestrous bitches, the plasma FSH concentration was significantly higher than the pretreatment level at 10, 20, and 30 min, whereas the plasma LH concentration was significantly elevated at 10 and 20 min. The maximal GnRH-induced plasma FSH concentration in the anestrous bitches did not surpass the lowest plasma FSH concentration in the OVX bitches, whereas the GnRH-induced plasma LH concentrations in the anestrous bitches overlapped with the basal plasma LH concentrations in the OVX bitches. In the OVX bitches, GnRH administration did not induce a significant change in the plasma FSH concentration, whereas the plasma LH concentration increased significantly at 10 and 20 min.In conclusion, the results of the present study indicate that in anestrous bitches GnRH challenge results in increased plasma levels of both FSH and LH, whereas in the OVX bitches, in which the basal plasma FSH and LH concentrations are higher, only a rise in the plasma LH concentration is present after GnRH stimulation. The results also suggest that a test to measure plasma concentration of FSH in single samples appears to have potential in verification of neuter status in bitches. #

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“…Thus, in humans glycosylated PRL (G-PRL) has been reported to be up to 20, 32 and 50% in plasma in different studies [2,4,16] while in the same biologic fluid from rats it was reported to be 60% [1]. The biological activity of G-PRL in some of the classi- not detected significant differences in PRL levels between overt and covert PSPT bitches [11,12,15,20,22]. Indeed, a universal serum PRL threshold for triggering pseudopregnancy in the bitch is unlikely to exist.…”

Section: Discussionmentioning

confidence: 97%

Heterogeneity of circulating prolactin in the bitch

et al. 2001

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-Different molecular forms of circulating prolactin (PRL) are known to occur in several species. As no such information was available in dogs, we assessed the molecular profile of circulating PRL in bitches. Pooled sera from covertly (CTRL) and overtly pseudopregnant (PSPT) diestrous bitches with high or low (> 10 or < 10 ng . mL -1 , respectively) serum PRL (measured by ELISA) were analyzed by Sephadex G-100 and Concanavalin A-Sepharose column chromatography. Four serum PRL fractions were identified and termed big-big, big (> 67 kDa), native (23 kDa) and fragmented (< 20 kDa) PRL. The percentages of these fractions were roughly similar in CTRL and PSPT animals, irrespective of their serum PRL levels (higher in PSPT than in CTRL bitches). A large proportion of glycosylated PRL (between 69 and 100%) was also detected in these sera. We conclude that in dogs, circulating PRL occurs in multiple molecular forms, whose relative abundance is comparable in covertly and overtly pseudopregnant bitches. prolactin / molecular variants / bitch / diestrus / pseudopregnancyRésumé -Hétérogénéité de la prolactine circulante chez la chienne. La prolactine (PRL) circulante se trouve, dans différentes espèces, sous plusieurs formes moléculaires. Compte tenu du fait qu'il n'existe pas d'information disponible chez le chien, nous avons décidé d'étudier le profil moléculaire de la PRL circulante chez cet animal. Nous avons utilisé des pools de sérums provenant de chiennes normales en diestrus (CTRL) ou pseudogestantes (PSPT), ayant des taux élevés ou bas de PRL (> 10 ou < 10 ng . mL -1 respectivement; dosages effectués par Elisa). Les sérums ont été analysés sur colonne de Séphadex G-100 et par chromatographie d'affinité, sur colonne de Sépharose couplée à de la Concanavaline A. La chromatographie sur G-100 a identifié quatre fractions de PRL, qui ont Reprod. Nutr. Dev. 41 (2001) 505-511 505

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Radloimmunological Measurement of Follicle Stimulating Hormone and Prolactin in the Dog

Abstract: ABSTRACT

Cited by 43 publications

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Suppression of luteal function in dogs by luteinizing hormone antiserum and by bromocriptine

Suppression of luteal function in dogs by luteinizing hormone antiserum and by bromocriptine

Basal and GnRH-induced secretion of FSH and LH in anestrous versus ovariectomized bitches

Heterogeneity of circulating prolactin in the bitch

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